Lux Arbor MLE raingauge was plugged up. I cleaned the funnel.

There were 9.9 mm of water in the funnel, that was discarded.

I added the heat flux plates to the tower 2 program.

Calibration coefficients and locations: on V116, 2H/2L mulitiplier 44.075 (east of tower), 3H/3L multiplier 37.262 (north of tower), 4H/4L muliplier 44.072 (south of tower). 1” below surface.

I updated the PAR sensor calibration to 6.5 uA/ (1000 umol/s/m^2)

Raised tower 2 to a height of 2.25cm above ground level. Will need to raise both towers on June 30 (next Monday). Checked sensor wires on tower 2. Did not get to RH sensor on Tower 1 (not reading).
Edit: 2.25m (not cm) - dw

Sequence LTER20250620S8BGC issues: All GC vials run sequentially, some samples submitted out of order in trays. Vials 41 and 57 were swapped (i.e. …39, 40, 57, 42, 43… and …55, 56, 41, 58, 59…) One other set of vials submitted out of order as follows: …105, 106, 111, 108, 109, 110, 107, 112, 113…

BCSE20250530060506130619S7S8S9S10BGC sequence had one exception: Series 10 had a note that vials 8 and 5 were swapped in the field. Therefore, for series 10, they were run in that order on the GC, i.e. …3, 4, 8, 6, 7, 5, 9, 10….

BCSE gas vial, tote prep - Sarah

Grind plants, pulverize soil - Sam,

Archive air-dried soil -

Pack CN - Brian

Data entry -

LTAR 15 gas sampling - Brian, Slater, Jon

LTAR Hydrosense - Trajana

BCSE gas chamber prep - Sarah

Woody maintenance -

Installed three heat flux plates (from Jiquan) at BAU2 field 1, tower 2. All on the V116, 2H/2L mulitiplier 44.075 (east of tower), 3H/3L multiplier 37.262 (north of tower), 4H/4L muliplier 44.072 (south of tower). 1” below surface.

Grind plants, pulverize soil - Olivia, Jon,

LTAR gas vial, tote prep, pack CN - Olivia

Filter BCSE soil extracts -

Archive air-dried soil - Slater

Dishes -

BAF subsampling - Trajana

Collect LTAR leachate - Sarah, Sam

Sticky traps - Slater, Brian

LTAR gas chamber prep - Trajana

Evacuate LTAR lysimeters. Clip pre-mow samples from ASP6 R5… 2x0.5m frame placed approximately in the center of each half of the plot (east and west halves), with the 0.5m frame ends oriented towards the east and west. Sorted to species in the field lab later. - Brian, Slater

Sticky traps - Jon, Sam

Sort ASP6 R5 plants to species - Slater, Brian, Trajana

Weigh dry tins, BCSE soil for extraction and moisture - Trajana, Olivia

Grind plants, pulverize soil - Sara, Olivia

Dishes - Jon

Pack CN - Brian

BCSE soil sampling, used bucket augers, one core/station to 25 cm depth, in G1-3 one core/row, one core centered halfway between rows, and one core halfway between the row and the center - Trajana, Sarah, Slater, Brian, Jon

Woody maintenance -

Grind plants and pulverize soil -

Dishes -

Reinstalled MDARD collars to R2,3,4 ASP and BAU corn for the MDARD project. Two collars on six fields=12 collars to capture Depression, Summit, and slope conditions with in and between row placement.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

LTER 8 gas sampling - Sam, Slater, Jon, Brian, Sarah

LTER Hydrosense - Alyssa

Replace short flags with regular tall station flags in LTAR corn and soybeans and BCSE G1-3 - Sarah, Brian

Mounted raingage to the horizontal bar on Tower 1 at approximately 1:30PM. Tower 2 is similar and Jiquan is also mounting on the instrument bar.

Removed ASP EC tower 1 raingage from remote tpost to horizontal bar on the tower (as is tower 2). I sent Jiquan a message and he agrees with the rain gage placement.

LTER gas vial, tote prep - Brian, Sarah

Grind plants, pulverize soil - Sam,

Dishes -

Lachat run LTER Lysimeter 20250310.

-Vial SF1-3 had no ‘analysis’ sample, used ‘archive’ sample. Vial CF1-3 has volume listed as below 20 ml, run as missing vial CF2-3.

LTAR 14 gas sampling - Sam, Jon, Slater

BCSE 10 gas sampling - Sarah, Brian

LTAR and BCSE Hydrosense - Trajana

LTER gas chamber prep -

Collect LTER leachate - Brian, Sarah

LTAR, BCSE gas chamber prep - Slater, Trajana

Sticky traps - Jon, Sam

Measure and filter LTER leachate -

Filter LTER extracts -

Dishes -

Acid washed dishes.

Weigh LTER soil for extraction and moisture - Sam, Jon

Grind plants, pulverize soil -

LTAR, BCSE gas vial prep -

Dishes

Evacuate LTER lysimeters - Trajana, Sarah

Sticky traps - Brian, Slater

Prepared Standards, reconditioned cadmium column.

Lachat run LTAR lysimeter 20241209

• Samples BAU2 R2 N and ASP2 R3 C had vials filtered, but had volume listed as 15ml, ran samples as vials had enough sample for 3 samples and most likely had a volume at or above 20ml.
• Sample ASP2 R4 S had no volume listed, ran as missing vial ASP2 R4 C.

Finished all 2024 Lysimeter samples - LTAR files not uploaded.

Sieve LTER soil -

LTER soil sampling, T1, 2, 3, 4, 5: too dry for push corer, sampled with bucket auger, took one sample (to 25cm depth) from each station 1, 2, 4, 5 (skip station 3), alternating in the crop row and between. T6-8 and the forest: sampled as usual with 2 push core samples per station. - Alyssa, Slater, Olivia, Brian, Trajana, Sam, Sarah, Jon

BCSE 9 gas sampling - Slater, Olivia

BCSE Hydrosense - Trajana

Tree maintenance - Brian, Jon, Olivia, Trajana

Acid washed dishes.

Grind plants, pulverize soil - Trajana, Slater, Sam

Dishes - Jon, Olivia, Trajana

LTAR 13 gas sampling - Brian, Sarah, Jon

LTAR Hydrosense - Trajana

BCSE gas chamber prep -

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTAR Lysimeter 20241125.

Grind plants -

BCSE gas vial, tote prep -

Pulverize soil -

Pack CN, dishes, data, BAF subsampling -

Replaced all reagent tubing on Lachat.

Lachat runs LTAR Lysimeter 20241031 and 20241114.

at 9:!5 EDT Kevin removed the ground sensors and rain gauge from tower 1 for fertilization. He disconnected the P1 wire from the rain gauge before moving the gauge so that it does not register additional tips.

sensors were re-installed at 15:30.

GC Sequence LTER20250606S7BGC had just a few samples submitted out of order: All vials run in numerical sequence on GC. Submitted vials out of order in trays: …232, 234, 233, 235, 236….

Collect LTAR leachate - Sarah, Jon

Sticky traps (reinstall T6) - Brian, Sam

LTAR gas chamber prep - Olivia, Trajana

Measure and filter leachate - Sarah, Jon

Dishes - Sam

LTAR gas vial, tote prep - Olivia, Trajana

Filter LTAR soil extracts - Sarah, Jon

Grind plants, pulverize soil - Trajana, Olivia

Weigh LTAR soil for extraction and moisture - Sarah, Slater

Grind plants, pulverize soil, archive air-dried soil, dishes, data, BAF subsampling -

Evacuate LTAR lysimeters - Trajana, Jon

Sticky traps - Sam, Brian, Jon

Lachat run LTAR Lysimeter 20240812. -Vial ASP1 R4 N has volume listed as not enough to filter, ran as missing vial BAU2 R4 S.

removed 2 sensors from T7R5 because they were blocking the bus.

Reconditioned cadmium column.

Finished dilutions from Lachat run LTAR Lysimeter 20240725.

Sieve LTAR soil -

Grind T7 REX 2023 plants and organize samples - Brian, Olivia

Pulverize BCSE 2022 deep core soil - Sam

BAF subsampling BCSE 2022 deep core soil - Jon

Dishes - Sarah

LTAR soil sampling -

Install ASP2, ASP5 tall station flags -

Fix tipping and fallen flags on BCSE and LTER - Slater, Trajana

I mistakenly connected the raingauge to P2 on Tower 1 when we lowered the crossarm on 2025-05-12. So there the rainfall info is missing since then.

I moved the connection to P1 this morning.

Restarted REX logger T7R1 since it was not sending any data.

Finish sorting ASP2 plants -

Dishes, data -

LTER 7 gas sampling - Slater, Jon, Sam, Sarah, Olivia, Trajana (Hydrosense)

Prepared Standards, Acid washed dishes.

Lachat run LTAR Lysimeter 20240725 - Too many dilutions for today, will run next week. Sample labeled ‘ASP3 R3 N’ should not exist, vial ASP4 R3 N missing so ran as missing vial, labeled incorrectly?

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

GC sequence LTAR202505220529S10S11BGC sample issues: All samples run on GC in sequential order. Series 10 vials submitted out of order: …80, 81, 86, 83, 84, 85, 82, 87, 88…. Series 10 vial 103 broke in field, so sample run is “dummy” sample of air. Series 11 vials submitted out of order: …6, 7, 11, 8, 9, 10, 7, 12, 13, 14, 19, 16, 17, 18, 15, 20, 21…. and …123, 124, 126, 125, 127, 128…

LTAR 12 gas sampling - Trajana, Jon, Sarah

BCSE 8 gas sampling - Sam, Slater

LTAR and BCSE Hydrosense - Alyssa

LTER gas chamber prep -

LTER gas vial and tote prep -

Sort ASP2 plants -

Measure and filter LTER leachate - Sarah, Slater

Filter LTER soil extracts -

LTAR, BCSE gas tote pre -

Sort ASP2 plants -

Collect LTER leachate - Slater, Sarah

Sticky traps, pull T6 for fertilization -

LTAR, BCSE gas chamber prep - Olivia, Trajana

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Lysimeters 20241107 and 20241216.

replaced the CR1000x on tower 2 with an older one from REX. Upgraded to OS 8.02.01. Watchdog errors are much reduced. Still see an occasional one, but about the same rate as tower 1.

Sieve LTER soil - Alyssa, Clara, Sarah, Trajana

Weigh LTER soil for extraction and moisture - Alyssa, Clara, Sarah, Trajana

Sort ASP2 plants -

LTAR, BCSE gas vial prep - Stacey

The tower 2 issue appears to be caused by the SDI12 subsystem.

I moved the EC100 power to the battery power block on the DIN rail and disconnected the power from the 12V and Ground ports from the datalogger. This reduced the amount of buzzing from the IRGASON and seems to eliminate the watchdog errors.

I detached the C3 connection to the CS650 sensors and all of the power lines to both the HYGROVUE and the CS650’s. Then added the power back one at a time. No watchdog errors as long as no SDI12 sensor was attached. I did not test the HYGROVUE with the C1 connection detached but if it had power it produced watchdog errors. oonnecting a CS650 with power to the C3 port resulted in watchdog errors for each of the CS650 sensors. I was able to set the addresses yesterday in between the crashes. So I think the communication is working.

Evacuate LTER lysimeters - Slater, Sam

Sticky traps - Sam, Slater, Jon

LTER soil sampling, very dry in T1-4 so sampling was difficult and took most of the day - Slater, Sam, Jon, Sarah, Trajana, Clara, Olivia

Tower 2 datalogger is crashing about every 30 seconds. Both with the flux tower 1 program and with the flux tower 2 program, so i don’t think it’s a programming error. Will check in the morning.

Grind plants - Olivia

On Tower 2 all the CS650 probes had an SDI12 address of 0.

• Connected the CPI ports of the VOLT116 to the datalogger so it can read from the VOLT116.
• Connected the data line block from the DIN block to C3 on the logger
• Updated the SDI12 addresses of the sensors to match the last digit of the serial number 4, 5, 6
• Updated the SDI12 addresses in the program to match the addresses on the CS650 sensors
• Updated the SDI12 address of the HYGROVUE sensor in the program.

Added a -1 multiplier to the PAR sensor reading until we reverse the leads again.

Reconditioned Cadmium Column.

Lachat run LTER Lysimeter 20240822 (Samples SF R2-3 and SF R2-6 may have been processed incorrectly. Vials from SF R2-3 have consistently had higher levels of NO3 than all other forest samples since installation, but a higher level was present in SF R2-6 for 8/22/2025.)

Measure and filter LTAR leachate, dishes - Jon, Sarah

Grind plants -

Sort ASP2 plants -

Sequence LTER20250523S6BGC notes: SF1-1 and SF1-3 were switched around at sampling time - see vial # notes on setup file sheets Vials submitted out of order, but run in sequential order on the GC (may or may not have anything to do with the first note). Out of order vials noted as follows: …127, 128, 129, 132, 130, 131, 133, 134… …147, 148, 153, 154, 155, 156, 149, 150, 151, 152, 156, 157… (guessing these correspond to SF1-1 and SF1-3 mixup) …241, 242, 247, 248, 245, 246, 251, 252, 249, 250, 243, 244, 253, 254, 255…

Installed G1-3 (except G1R2 and G1R3, those two chambers were accidentally left in during herbicide application on 5/29) chambers just prior to gas sampling BCSE 7 - Slater, Brian

BCSE 7 gas sampling - Olivia, Sam, Andie (Hydrosense)

Collect LTAR leachate - Sarah, Jon

Measure and filter leachate, dishes - Sarah, Jon

Sort ASP2 plants - Brian, Olivia, Sam, Andie

Grind plants - Slater

Filter LTER soil extracts - Alyssa, Sarah

Lachat run LTER Lysimeter 20240801.

Evacuate LTAR lysimeters - Brian, Sarah

LTAR 11 gas sampling - Sam, Slater, Jon, Andie (Hydrosense)

ASP2 forage sampling ahead of harvest, Stations 1, 2, 3: 0.5mS, 0.5mW to NE corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. Clip all plants rooted inside the frame as close to the ground as possible without collecting litter or soil. Sorted later in the field lab…MEDSA, TRFPR, CICSP, DACGL, BRSNA, remainder label UNSRT - Sam, Slater, Brian, Jon

Weigh T3, T4 soil for extraction and moisture - Sarah, Andie, Olivia

LTAR gas vial, tote prep - Olivia

Grind plants -

Subsample 2022 BCSE deep core soil to send to BAF - Andie

Dishes, data -

Sticky traps - Sam, Slater, Brian, Jon

LTAR gas chamber prep - Alyssa, Sarah, Jon

Prepared standards, prepared 1M KCL.

Acid washed dishes.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

LTER T3, T4 station (4 cores/station, 2 in the wheat row and 2 between wheat rows) and strip (4 cores spread across each strip avoiding transects, foot paths, traps and gas chambers) soil sampling - Sarah, Andie,

Sticky traps (first check for T3, T4, prairie strips; reinstallation for T1, T2 as they were pulled for fungicide over the weekend) - Slater, Brian, Jon, Sam

Lachat run LTAR Soil 20241120, 20241218.

All 2024 LTAR, LTER, and GLBRC soil samples processed - LTAR samples not uploaded.

Sequence BCSE2025043005140522S4S5S6 had a couple tech notes for series 6: Vial 49 was lost during sampling, so a replacement vial was used to collect the sample - this was run in sequential order on the GC. Ambient air samples were not collected - empty vials were run on the GC anyway for continuity of the run.

Sieve T3, T4 soil - Sarah, Andie

WPS - Sam

LTER 6 gas sampling - Olivia, Brian, Trajana, Sarah, Jon, Slater, Andie, Sam

LTER Hydrosense - Trajana

Filter BCSE soil extracts, dishes -

Grind T7 REX 2023 plants -

Lachat run LTAR soil 20241028. Reconditioned Cadmium Column.

Sieve and weigh BCSE soil for extraction and moisture -

LTER gas vial and tote prep -

Finished Lachat run GLBRC Scale up Marshall 20241202, 20241216 - Blanks from 20241202 showed NH4 contamination in KCL (M1, M2, M4), not uploaded.

BCSE 6 gas sampling - Brian, Andie, Slater (soil, 3 cores/plot)

LTAR 10 gas sampling - Sarah, Jon, Sam

BCSE and LTAR Hydrosense - Trajana

LTER gas chamber prep -

Measure and filter LTER leachate -

Filter LTER soil extracts - Trajana, Andie

Grind plants - Olivia

Lachat run GLBRC Scale up Marshall 20241202, 20241216 - half of run completed due to peek tubing malfunction. Replaced with new tubing, will finish rest of run tomorrow.

set up datalogger on tower 2. Enabled ethernet port, and loaded program. Tested Loggernet communication.

Collect LTER leachate - Olivia, Brian, Jon, Sarah

BCSE and LTAR gas chamber prep - Slater, Sam, Trajana, Andie

Sticky traps (first cards deployed in T5-7 and forest plots) - Sam, Slater, Brian, Jon

Weigh LTER soil for extraction and moisture - Sam, Slater

Gas vial and tote prep, dishes - Sarah

BAF subsampling (2024 BCSE peak plants from archive jars) - Trajana

Grind plants - Andie

Prepared standards, prepared 1M KCL.

Lachat run GLBRC Scale up Lux Arbor 20241202, 20241216 - Blanks from 20241202 showed contamination in NH4 (L1 and L3 samples), not uploaded.

Reconditioned Cadmium Column.

Evacuate LTER lysimeters - Trajana w/Jon, Andie w/Sarah

Sticky traps (deploy first sticky cards in T1-4 and prairie strips) - Sam, Slater, Brian, Jon

GC Run Sequence LTAR202505080515S8S9BGC notes: Dave dropped and broke vial 38 for Series 8. Replaced with dummy sample (lab air, not pressurized).

Site-specific training for techs - Stacey, Alyssa, Slater, Brian, Olivia, Trajana, Sam, Andie, Sarah , Jon

Sieve LTER soil -

Grind plants -

BAF subsampling (2023 BCSE plants) - Brian

LTER soil sampling, 2nd May -

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.