Noticed an issue with inconsistent check standards on LTAR202504100417S4S5BGC run. Some checks made before, some after, a nitrogen tank change-out. (N2 used in making up standards.) High standards, with no N2 addition, appear to be fairly consistent across the last 3 sample runs. A separate standard/check std run was completed after the LTAR run to test for this issue. This standard run would likely be better to use with the LTAR run. Standard run was STD_TEST20250424BGC.

LTAR202504100417S4S5BGC sample run on GC had vials submitted out of order on trays. All samples were run on GC in numerical order. Samples submitted out of order as follows: …4, 5, 9, 7, 8, 6, 10, 11, 12… and …135, 136, 140, 137, 138, 139, 141, 142….

BCSE202503310416S2S3BGC sample run on GC had a small set of samples submitted out of order on the trays. All vials were run on the GC in numerical order. Vials out of order on trays as follows: ….56, 57, 59, 58, 60, 61, 62, 63…..

GC run LTAR202503260403S2S3BGC had several vials mixed up in one series 3 tray received from the techs. All vials run in sequential order on the GC. Here is the order presented in the trays for series 3: …23, 24, 25, 46, 47, 48, 29, 42, 43, 44, 33, 38, 39, 40, 37, 34, 35, 36, 41, 30, 31, 32, 45, 26, 27, 28, 49, 50, 51, 52, etc….

GC run BCSE20250307S1BGC had one series of vials submitted out of numerical order. All vials run on the GC in numerical order, but submitted series were …60, 62, 61, 63, 64, 65, etc… (so just 61 and 62 submitted out of order, but subsequently run in numerical order).

GC run LTER20250306S1BGC: samples 241 and 242 were switched out of order in the trays submitted by the techs (likely just an oversight). Vials all run in numerical order on GC.

GC run LTAR20250211S30BGC vial order issue. All vials run in numerical order. Vials submitted in trays out of order as follows: …103, 104, 108, 105, 106, 107, 109… and …111, 112, 114, 113, 115, 116, 117…

LTAR2024121720250116S28S29BGC Series 28: techs noted that vials 33-36 were sampled in order as 36, 33, 34, 35 and that vials 38 and 39 were swapped. Therefore, vials run in numerical order until that point, and then ordered as noted in sequence and here: vials ….31, 32, 36, 33, 34, 35, 37, 39, 38, 40, 41, 42….

Series 29: all vials run in numerical order, no tech notes, but found samples out of order in trays - …139, 140, 144, 141, 142, 143, dup1…. To reiterate, these samples were reordered to run in numerical order on the GC, i.e. …139, 140, 141, 142, 143, 144, dup1, etc…

LTER2024121020250110S14S15BGC Both these series have shortened sample sets with vial numbers as follows: Vials 1-112, 157-172, 185-200, 237-252.
Series 14 had vials 12 and 20 switched in the submitted trays. These were run in numerical order on the GC.

BCSE202410071104S16S17BGC run had some submitted samples out of order. All samples run on GC in numerical order. Series 16 samples out of order, submitted in following sequence: …10, 11, 16, 13, 14, 15, 20, 17, 18, 19, 12, check, 21, 22….

GC Run SWFG series39 10/8/2024 2 vials out of order at sampling: 14 and 15 were switched at sampling, so will be run in the order …12, 13, 15, 14, 16, 17…. GC Run SWFG series41 10/23/2024 2 vials out of order at sampling: 93 and 94 were switched at sampling, so will be run in the order …92, 94, 93, 95, 96….

GC run of LTER gas samples, sequence LTER20240913S11BGC Vials submitted in trays out of order as follows: …115, 116, 120, 117, 118, 119, 121, 122, 123, 124, 128, 125, 126, 127, 132, 139, 142, 129, 136, 135, 138, 140, 131, 134, 137, 144, 143, 130, 141, 145, 146, 171, 172, 149, 150, 167, 168, 153, 154, 163, 164, 157, 158, 159, 160, 161, 162, 155, 156, 165, 166, 151, 152, 169, 170, 147, 148, 173, 174, 175, 176, 177, 178, 179, 184, 180, 181, 182, 183, 185, 188, 187, 186, 189, 192, 191, 190, 193, 194, 195, 196…. All vials run sequentially on GC. Looked at field sheets - appears that vials up to 171/172 were likely sampled in proper order, and just jumbled up on trays. It is possible vials sampled in correct order after 172, but there are some field sheet issues with those, along with being submitted out of order.

LTER Series 10 8/20/2024 File: LTER20240810S10BGC Note from Techs on sampling vial error: “Start 1 and 2 out of order vials 1-40 (1=21, 2=22)” May be more notes on sampling sheet? A number of vials submitted out of order. All vials run in sequential order on GC. Vials out of order in tray: …41, 42, 55, 56, 45, 46, 51, 52, 49, 50, 47, 48, 53, 54, 43, 44, 57, 58, 59….

BCSE202407190731S12S13BGC: All vials run in consecutive order. Vials submitted by techs in this order for Series 12: 1, 18, 3, 4, 5, 14, 7, 8…. ….12, 13, 6, 15, 16, 17, 2, 19, 20, 21…. all other vials in order.

LTER20240724S8BGC GC sequence: Samples all run in sequential order on GC. Samples somewhat mixed up in trays presented by techs. Tray vial order as submitted to lab noted here: 145, 146, 171, 172, 149, 150, 167, 168, 153, 154, 163, 164, 157, 158, 159, 160, 161, 162, 155, 156, 165, 166, 151, 152, 169, 170, 147, 148, 173, 174, 175, 176, … Then in order till 252, 253, 262, 263, 264, 257,258, 259, 260, 261, 254, 255, 256, 265, 266, ….

SWFG2024071607180722S24S25S26AGC sequence saw GC reboot and restart around sample vial 194 of the “B” round (N2O/CO2 analysis). N2O peak cut off and did not integrate. After sequence completion, re-ran sample group vials 191-194 under sequence name SWFG2024071607180722S24S25S26AGC_B. Results fairly consistant, and now have N2O data for sample vial 194.

LTER20240711S7BGC - samples 296 and 292 swapped in sample tray submitted by techs. All samples run in sequential order on GC.

Notes on GC run LTAR202406110618S12S13BGC : S12 - all samples run in numerical order, samples 7 & 8 were switched in field tray, samples 141 and duplicate 1 were swapped in field tray - order corrected in GC tray and run in numerical order S13 - note from field tech: vials 133-136 swapped with 141-144. All samples run in numerical order on GC

Running 3 series of GLBRC gas samples. Notes on Series 6 from Techs - Vials 1-40 swapped, i.e. vials 1-20 are actually vials 21-40 regarding site sampling. Series 7 no notes. Series 8 - a number of vials out of order. All samples run in numerical order. Series 8 vial order in trays: 1-40 ok, then 41, 42, 59, 60, 45, 46, 55, 56, 49, 50, 51, 52, 53, 54, 47, 48, 57, 58, 43, 44, 61, 62, 63,…

SWFG Gas sampling notes: Series 10, Vials 1 and 25 swapped at sampling time - noted on field sheet and run on GC in that order (i.e. 25, 2, 3, 4, 5… 24, 1, 26, 27, etc.) Elias’ previous samplings of Blocks 2 & 4 may have been completed in opposite order of sheet - moving west to east instead of east to west in each instance. He corrected that on series 11 sampling. Will have to confirm, but mirrored samplings may have occurred on series 8 and 9.

GLBRC Series 4 vials. Note from techs that vials 1 and 21 were swapped (i.e. 21, 2, 3, 4, 5…20, 1, 22, 23, etc.). Still run on GC in consecutive order (1, 2, 3, etc…)

BCSE Series 3 05/07/2024 - Vial 79 dropped and broke. Replaced vial with nitrogen blank.

LTAR Series 8 vials out of order. All vials run on GC in sequential order. One set of vials out of order and submitted in the following sequence: …84, 85, 88, 87, 86, 90, 91, 92…

LTAR202404230430S6S7BGC LTAR series 6 vial mixup. All samples run in numerical order. Tech order in trays/racks: …25,26, 28 , 27 ,29,30…., ….48, 73 ,50,51,52, 77 ,54,55,56, 61 ,58,59,60, 57 ,62,63,64, 49 ,74,75,76, 53 ,78,79,80… LTAR series 7 vial mixup …108,110,109,111,112….

LTER20240425S2BGC GC run Appears that vial 11, std 3.0 is off/incorrectly made, compared with all the other standards.

LTAR Series 2 and 3, datafile LTAR2024403280404S2S3BGC notes: Series 2 had a number of vials out of order received by techs. All vials run in order on GC. Vial order from techs in trays: …95, 96, 117, 101, 99, 100, 121, 102, 103…. then …115, 116, 97, 118, 119, 120, 98, 122, 123…. Series 3 only had one vial pair out of order - 57 and 58 were swapped in the tray. All vials run in numerical order.

First LTAR gas chamber sampling run on the GC. All samples run in numerical order. Rack order as submitted by techs had these two issues: vials 98 and 101 were swapped, and vials 135 and 131 were swapped. (Meaning order submitted was …96,97,101,99,100,98,102… and …129,130,135,132,133,134,131,136…. respectively.)

LTER20240306S17 GC run notes: Vial 20 broke in transport. Dave refilled vial with N2 and ran in series - should have blank results for vial 20. All samples run in numerical order, though received vials 163 and 164 swapped in tech’s sample tray.

Vial error on BCSE Series 20 run. Lost nitrous oxide values for vial 66, but was able to continue run uninterrupted otherwise. Setup a separate run that just included a blank vial and vial 66. Transferred those N2O values to the BCSE Series 20 run results for vial 66. CO2 values slightly different, but kept the original values. Rerun saved under same name with a “-A” added at the end of directory/filename.

Found an error in naming a recent run. January, 2024 collection and run for BCSE samples was recorded as Series 18, when it should have been Series 19. This has been corrected in the data file storage on BenGC computer. Updated data directory name to \BCSE20240110S19BGC 2024-01-10 13-09-04. Excel results file found in that folder was also corrected, changing all S18 samples to S19. File sent to Kevin for approval while error corrections are fresh in our minds…

Vial notes for GC run LTER20240206S16BGC.PRP: All vials run in numerical order. Vials swapped in trays received from Techs: 46 & 47, 50 & 51, 66 & 67. i.e. Vials received in this order 44, 45, 47, 46, 48, 49, 51, 50, 52, 53… but run in this order 44, 45, 46, 47, 48, 49, 50, 51, 52, 53….

Combo sampling run of LTER/GLBRC samples - Everything run in order until end of BCSE samples. Tech note that Ambient 1 was actually Duplicate 2. So last 8 BCSE samples physically run in this order: Dup1, Amb1, Dup3, Dup4, Dup2, Amb2, Amb3, Amb4. The sample queue in the software is Dup1, Dup2, Dup3, Dup4, Amb1, Amb2, Amb3, Amb4.

GC run corrections LTER Series 13, collected 11/2/2023. Filename LTER20231102S13BGC.PRP Vials all run in numerical order on GC analysis. Some vials submitted were out of order in the racks. Out of order vials: …176, 177, 182, 179, 180, 181, 178, 183, 184…

LTER Series 12 (10/06/2023) GC analysis notes: All vials run in numerical order. Vials 16 and 14 switched in sampling tray. Vials 73 and 93 switched in sampling tray. Ambient 2 vial broke - replaced with a blank sample (N2). During run second pass, vial #17 at position 31, the analysis paused with the vial lost from the autosampler rack (found on chair). Vial replaced and analysis started back up - GC made CO2 injection and continued on with the run.

GC run of LTER Series 11 notes:
Samples 183 and 175 were switched in trays submitted by techs. All samples run in numerical order on GC. CO2 analyzer did not have the gas turned on for initial run. Part “B” of the run was started over after gas flow was reset. So, the first set of standards are likely bad for this portion of the run - only use the 2nd set of standards for calibration.

Replaced standard cylinder from GC lab. Attached are old and new files with calculated concentrations.

The instrument malfunctioned/stopped during the holiday weekend and was not noticed upon return. Thus, much of the second loop, N2O and CO2 was not analyzed. CH4 values will be kept. Caught this in the flux approval phase by KK in Feb. 2024.

Patrick Wright took the ECD from the LTER Shed Shimadzu for disposal. I removed the detector prior to Patrick’s arrival. GC now has an equipment release form filled out by Patrick. Need to clean instrument and then ready for Surplus (or disposal).

Removed ECD from LTER Shed Shimadzu GC for pickup on Wednesday 5/3 by Patrick Wright.

Setup and started Ben GC series 13 LTER. Series 12 vial caps were not tightened fully so many may have leaked. Need to review S12 and decide whether to use or toss.

Changed N2 and H2 cylinders lab GC.

Kevin and Dave Deep Core afternoon. Training for Dave and collected T3R4 All, T3R5 S1 and 2. Worked on samples for Debasish N2O + dilution on 72 samples.

Running first half of J. Jones CUE samples.

LTER S9 GC samples on GC.

Run of samples for Evans Lab (Holly) kk

Second needle bent on Ben GC running efix samples. Throw out old caps.

GC run, LTER series 7, 20220629-Cathy

GC run, LTER series 5, 20220602-Cathy

GC standards and checks-Cathy

Restarted ANNA GC today. Program not connecting to instrument, but possibly due to Gerstel valve switch. KK

GC run, LTER series 4, 20220519-Cathy

GC run, BCSE soil gas series 4, 5 and 6, 20210504, 20210518, 20210526-Cathy

GC run, LTER series 3 20220505-Cathy

waiting on new hydrogen cylinders. Have another new cylinder that has a locked up cap.

GC run, LTER gas series 2, 20220420-Cathy

Make standards and checks for LTER gas-Cathy

GC run, LTER 20220407-Cathy

GC run, LTER gas series 16, 20220309-Cathy

GC run, LTER series 15, 20220209.-Cathy

GC run, 2021 BCSE samples, series 18-Cathy

GC run, LTER gas series 14, 20220112.-Cathy

GC run, LTER 20211014 series 11-Cathy

GC standards and checks for Kevin-Cathy

GC run, Grant\’s samples-Cathy

Found needle bent and y-axis rail in an odd position yesterday. reset unit, tried again and the same thing occurred with an \”unable to sample 1000ul error\” message. Changed bungees and needle same. Then ran without syringe/plunger in unit. Worked fine. Added back in new syringe with no needle. Waiting. Ran fine. Added needle back now running as of 2PM

GC run, LTER series 8 20210714-Cathy

GC run, LTER gas series 7, 06/29/2021

GC run, Grant\’s samples (192)-Cathy

GC run LTER gas series 6-Cathy

GC run, LTER gas series 6, 20210616.-Cathy

GC run, LTER series 5, 6/2/2021-Cathy

Added T2 samples and started GC run, (LTER series 4 was set up but didn\’t get started last week)-Cathy

GC run, LTER series 4, 20210519-Cathy

GC run, LTER series 3, 20210504-Cathy

GC run, LTER 20210422-Cathy

GC run, LTER series 14-Cathy

GC run, LTER gas series 13-Cathy

GC run, LTER series 12 20210107-Cathy

GC run, LTER series 11-Cathy

GC run, LTER series 10-Cathy

Sampled aphid tower, ran half of soil incubation gas samples for Carmella.

GC run, LTER series 8-Cathy

ECD inspection JON WILL ALSO PICK UP AGILENT 25760 ECD in room 333. We have salvaged 5890s and RETURNED ECDs from 5890 GCs in lab 330 to Agilent per Stieglitz. Note former 5890 ECDs tested and shipped back to Agilent for disposal in 11/18. Then 5890s salvaged after passing wipe test. Kahmark, Kevin Fri 11/2/2018 9:37 AM TO: lsca-fieldrepaircenter@agilent.com Hello, Please add ECD L7845 to this shipment. I will need RMA numbers which I didn\’t see for M1363 either. Wipe tests are complete, detectors are capped and plugged. Thank you, Kevin Kahmark Hi Andy, We have two GC\’s in the lab that were just approved by the radiation health physicist for salvage. Both of these units had ECDs and were cleared of any isotopic contamination via swab tests sent to Jon Stieglitz for analysis. I\’ve attached his approval form to each GC also and would like to know where I can put these units (downstairs hallway?). These forms are actually to accompany the ECDs during shipment back to Agilent. You can remove these forms from the GC when you see them (not necessary on the GC) but included for your records. jAdditionally, I\’m working with Jon to ship the ECDs for disposal. These will be sent back to Agilent under the approved dangerous goods shipping authority (TSCA protocol staff member) in Jon\’s office or in EHS. I will call Jon over the next day or so to get more information. Thanks, Kev

GC run, LTER series 6 7/22/2020-Cathy

Ran samples for both Grant and Caro last week. Now running Allison Bressler samples collected on LTER study.

Downloaded results from Grants trial, made calculations and sent on to him. FLAVO1 on Ben GC.

GC run, LTER series 4-Cathy

Installed shelters at Lux Arbor. Injured foot, still need to add RH sensors to site. Fertilized by Todd on approximately 5/11. Shatterbox requires new fuses at a minimum. GC in the lab restarted on 5/18, checked on 5/19. Ready to run samples.

GC run, LTER series 13-Cathy

GC run LTER 20191219-Cathy

Make set of GC standards for Kevin.-

GC run, Corinn\’s samples-Cathy

Delivered swabs for both U25760 and new U36188 ECDs to EHS in person yesterday. Notified Jon that new ECD was installed and ready to use.

Spoke with Jonathan Stigleitz at EHS and we agreed that they would handle the return of the BEN GC ECD S/N U25760 Agilent. Email from Jon attached. Hi Kevin, Since we have to come down to survey and calibrate instruments we will just plan on picking it up and bringing it back to campus when we have someone to come there. That means we will take care of the disposal costs. It’s a little more expensive this way but we will plan on that. I will run the wipes and get the results to you today. You will just need to keep the old ECD properly labeled in a secure rad area until we are able to pick it up. Jonathan Stieglitz Radiation Health Physicist Office of Environmental Health & Safety Michigan State University 4000 Collins Rd, B20 Lansing, MI 48910 Wk: (517) 884-0502 Email: stieglitz@ehs.msu.edu http://www.ehs.msu.edu Map: https://www.google.com/maps/@42.691637,-84.500585,17z

Gleason reel on Ben GC snapped. Repaired for now. Ordering replacement.

Agilent came in and installed a new ECD and the adapter with weldment. Lost parameters on Licor and GC for some unknown reason. Will bring used ECD swabs to EHS on 1/8/20, then send back ECD via FedEx. Rest Licor DAC settings to 0-5vdc and 0-10000 values. Working well as of 1/7/2020.

GC run, Corinn\’s samples-Cathy

GC run, LTER series 1, 20191106-Cathy

GC run, arctic samples-Cathy

GC run, LTER series 10, 20191008.-Cathy