Projectlog Entries List

Finish sort SF1 ANPP, field lab clean-up - Alyssa, Jon, Emily, Glori, Olivia

Sort SF1 ANPP - Emily, Jon, Glori, Olivia, Alyssa

Sort SF2, SF3 ANPP - Stacey

Sort SF1 ANPP - Olivia, Jordyn

Sort SF1 ANPP - Alyssa, Jon, Emily, Jordyn, Glori, Olivia

Pack LTAR 2024 plants and LTER 2024 RR CN - Bella, Jordyn

Weigh LTER 2026 plants - Olivia

Sort T6 ANPP - Alyssa

Pack LTAR 2024 plants CN - Olivia

BCSE S13 S14 S15 GC run had some samples submitted out of order.
S14 had vials 19 and 20 switched in the field racks. S15 had four out of order submitted as follows: vial 52, 50, 51, 49 All samples run sequentially on DawnGC

DawnGC sequence LTAR-S21 had a few samples submitted out of order in field trays. Order submitted as follows: ....82, 83, 88, 85, 86, 87, 84, 89, 90,.... All samples run on DawnGC in sequential vial order.

LTAR gas vial, tote prep -

Finish sorting T7 ANPP - Olivia, Jordyn, Glori, Alyssa, Jon, Stacey

Sort T7 ANPP - Alyssa, Glori, Bella, Jordyn, Emily, Jon, Olivia

Pack CN - Jon

Sort T7 ANPP - Alyssa, Glori, Bella, Stacey

Data entry - Glori

LTER gas vial, tote prep - Stacey, Jordyn

Weigh LTER plants - Sam pm

Data entry - Jon pm

Pack CN - Bella pm

GC run on Dawn GC: LTAR, Series 19, 07/23/2026. Two vials submitted out of order - 40 before 39. These were switched to proper order on the GC rack and all vials run sequentially. In addition, there was an issue with LTAR Series 18 being stuck on "acquiring" at the very last standard in it's sequence. This caused an issue with loading the Series 19 run, until the Series 18 was aborted for the last standard, and the run cleared from the Run Queue. I think everything worked out, but there may be no data for that last standard on series 18.

LTAR Series 18, 07/20/2026 GC run. Two samples submitted out of order. All samples run sequentially. Sample vials 80 and 79 were swapped in racks submitted for analysis (i.e. vials ordered ...76, 77, 78, 80, 79, 81....).

LTER Series 8 06/26/2026 GC run had some samples submitted out of order. All samples run in sequential order, but these vials submitted out of order (as listed): ...220, 224, 222, 223, 221, 225....

Sort LTER T3/T4 Prairie Strip ANPP plants - Alyssa, Glori, Jordyn AM/PM

Dishes - Sam AM, Jon PM

Sort T3/T4 Prairie Strip ANPP - Alyssa, Jon, Glori, Bella, AM, Alyssa, Sam, PM

Dishes - Sam, Jon

Sort T3, T4 prairie strip ANPP plants - Sam, Jon, Emily

Sort T3, T4 prairie strip ANPP plants - Alyssa, Olivia, Jon

Sort T3, T4 prairie strip ANPP plants - Alyssa, Glori, Stacey

LTER gas tote prep - Stacey

WPS - Emily

Sort T3, T4 prairie strip ANPP plants - Alyssa, Brian, Olivia

Filter T3, T4 T21 soil extracts - Glori, Jon, Emily

LTER gas vial prep - Stacey

Prepared 1M KCL

Weigh T21 soil from T3, T4 stations and strips - Brian, Bella, Glori

Finish pooling T21 soil - Alyssa, Glori, Emily

Sort T3, T4 R1 prairie strip ANPP - Sam, Jon, Brian, Emily, Alyssa, Glori, Stacey

BCSE gas vial and tote prep - Stacey

Pool T21 soil bags A and B from T3, T4 stations and prairie strips -

Acid washed dishes.

LTAR gas vial and tote prep - Sam, Jon

Measure then filter LTAR leachate, filter LTAR soil extracts, dishes - Glori, Bella, Sam

Sort ASP6 ANPP plants - Stacey, Alyssa, Jon, Emily

Weigh LTAR soil for extraction and moisture -

Sort ASP6 ANPP plant samples - Stacey, Jon, Sam, Emily, Brian, Bella, Glori, Jordyn

Prepared 1M KCL, prepared standards. Acid washed dishes. Lachat run 2026 July LTER T7 soil (Lau lab).

Lachat runs GLBRC soil 20260424 and 20260622.

Sieve LTAR soil samples -

Sort ASP6 ANPP -

Sort T8 ANPP - Alyssa, Stacey, Jon

Filter LTER T21 soil extracts - Brian, Jordyn

Prepared 1M KCl

Sort T8 ANPP plants - Alyssa, Stacey, Jordyn, Jon, Olivia, Bella, Glori, Sam

Weigh LTER T21 soil samples for moisture and extraction - Brian, Bella

LTER gas vial and tote prep. WPS training - Emily

Measure and filter LTER leachate, dishes - Jon, Jordyn

Start sorting T8 ANPP -

Sort T5 ANPP - Brian, Emily, Jordyn, Olivia, Stacey

Lachat run LTER Lysimeter 20260402. Notes from run:

• Three vials labeled T3 R3 1, 2, and 3 were ran as T5 R3 1, 2, and 3. Flasks for T3 R3 and T5 R3 were swapped when evacuating samples. Weights were adjusted when entering in data file to the correct location, but vials were sampled incorrectly.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

LTER Grad Fellow Symposium

Pack CN - Glori

Data entry - Emily, Jon

LTER field lab to do list (cleaning, organization, dump old samples) - Jordyn, Sam, Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Replaced Cadmium Column, acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopore H2O.

Lachat run LTER Lysimeter 20260319. Notes from run:

• Vial labeled T2 R4 1 should not exist, not enough volume listed. Ran as Vial T3 R4 1, that was not present and should exist.
• Vial T CF R2 1 missing from box, not run (no data notes)
• Vial T6 R3 3 present, should not exist. Not enough volume in vial to run.

Weigh LTAR plants - Jon, Jordyn

Sort ASP1 forage - Emily, Brian, Bella, Jon, Sam, Olivia

Pack CN - Sam, Bella

Dishes, lab clean-up - Jordyn, Glori

NAS vial prep and data entry - Olivia

Prepared Standards.

Lachat run LTAR Lysimeter 20260616. Notes from run:

• Vial labeled ASP2 R1 N should not exist, not enough volume. Ran as vial ASP2 R2 N, that should exist and is missing.
• Vial ASP6 R2 S was missing from box and should exist, not run.

Measure and filter LTAR leachate, filter LTER soil extracts, LTAR and BCSE gas vial prep - Jordyn, Glori, Emily, Brian

Sort ASP1 forage - Sam, Jon

Sieve LTER soil -

Weigh LTER soil for extraction and moisture - Alyssa,

Sort ASP1 forage, empty ovens - Alyssa

Prepared 1M KCl

Make new LTER gas vials; all previous vials are awaiting analysis due to troubles with the old GC and switch over to a new GC - Alyssa, Olivia

Measure and filter LTER leachate - Sam, Jordyn, Glori

Reshake then filter LTER T3, T4 soil extracts - Emily, Brian

Prep LTAR gas vials, totes - Jordyn, Glori

Weigh T3, T4 soil for extraction, moisture and T21 whirlpaks (90-100g/whirlpak) - Olivia, Brian, Jordyn

BCSE gas tote prep, dishes - Jordyn, Brian

Dishes - Jordyn

BCSE new vial prep - Glori

Field lab clean-up - Brian

Dishes - Jordyn

Measure and filter LTAR leachate - Sam, Glori

Filter LTER soil extracts - Jordyn, Jon

Weigh LTER soil for extraction, moisture and T21 incubation - Glori, Jordyn, Brian around 11

BCSE and LTAR gas vial prep - Jordyn

Sieve LTER soil - Sam, Emily

Finish prepping LTER gas vials and totes - Brian, Bella, Jordyn, Olivia, Sam

Prep more NAS gas vials - Olivia

GHG talk from Kevin - Alyssa, Bella, Brian, Emily, Glori, Jon, Jordyn, Sam

Weigh and filter LTER leachate - Olivia, Jordyn

Filter LTAR and BCSE soil extracts - Glori, Brian

Prepared 1M KCL.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTAR Lysimeter 20260519. Notes from run:

• Samples ASP1 R4 C, S, and ASP3 R3 C were not present, not analyzed.

Weigh LTAR and BCSE soil for extraction and moisture - Glori, Jordyn

BCSE gas vial, tote prep - Glori

Grind LTAR plants - Brian

Pack CN - Jordyn

Grind plants - Glori, Sam

Sieve LTAR and BCSE soil -

Pack CN - Olivia

Archive LTER air-dried soil -

Dishes - Sam

Pack CN - Brian, Emily

Grind plants - Emily, Olivia

Grind LTER 2026 plants - Jordyn, Jon

Pack CN - Glori

Dishes - Sam

Data - Emily

Measure and filter LTAR leachate - Brian, Jordyn

Filter LTER soil extracts - Jon, Glori

Pack CN - Jon

Grind LTER 2026 plants - Sam

BCSE gas vial, tote prep - Glori

Weigh 2nd June soil for extraction and moisture - Jordyn, Glori

Grind LTER plants - Alyssa train Jordyn and Glori

Pack CN - Jordyn

Prepared 1M KCL. Replaced all Lachat reagent tubing.

Data entry - Brian

CN packing - Sam

Dishes - Glori

Weigh plants - Jordyn

LTER gas vial, tote prep - Glori

Weigh plants - Jordyn

LTER field lab clean-up, organization -

Measure and filter LTER leachate -

BCSE gas vial and tote prep -

Filter NAS soil extracts for Olivia -

Field lab cleaning and organization - Brian, Jon, Emily, Sam, Glori, Bella, Jordyn

Pack CN - Jordyn, Bella

Weigh LTER T3, T4 cover crop plants, then LTAR ASP2 - Bella, Brian, Jordyn

Data entry - Brian, Jordyn

Dishes - Glori

Weigh soil tins - Jordyn

Pack CN - Emily, Olivia

Pack CN - Jon, Sam

LTAR gas vial, tote prep - Glori

Dishes - Jon, Jordyn

Sort ASP1 plants - Brian, Emily

Sort ASP1 plants - Olivia am/pm

Measure and filter LTAR leachate - Jordyn, Glori

Filter LTER Soil extracts - Emily, Bella

Prep BCSE gas vials, totes - Emily, Bella

Weigh LTER soil for extraction and moisture - Glori, Sam

Dishes - Jordyn, Glori

Prepared 1M KCL. Acid washed dishes.

Measure and filter LTER leachate - Alyssa, Jon, Sam

LTER gas vial prep - Jon

Prepared 1M KCL.

Weigh LTAR soil for extraction and moisture - Brian, Olivia

Field lab clean-up, dump old samples, put soil (LTER 4/6/2026 and T3, T4 5/18/2026) out to air dry, organize sticky trap binder, weigh dry soil tins - Jon, Olivia, Emily, Jordyn, Glori, Brian

Dishes - Jon, Emily

Finished grinding SBE plants - Sam

BCSE gas vial, tote prep; WPS training - Jordyn

LTAR gas vial, tote prep - Glori, Olivia

Measure and filter LTAR leachate - Jordyn, Bella, Alyssa trained

Filter LTER T3, T4 soil extracts - Glori, Brian

Grind SBE - Sam

Data entry - Olivia

Prepared 1M KCL

Weigh T3, T4 soil for extraction and moisture - Alyssa, Bella, Jordyn, Glori

WPS site-specific training with Brook on Zoom - Glori, Bella, Jordyn

Safety training - Jordyn

Dishes, data entry - Glori

Onboarding, training - Jordyn (first day)

Grind SBE plants - Jon

Sieve LTER T3, T4 soil - Emily, Jon, Sam, Brian, Bella, Glori

Field lab clean up - Bella, Glori, Brian

Dishes, weigh dry tins - Olivia

WPS Zoom - Bella, Glori

Sticky trap (re)training on Teams - Emily, Olivia, Sam, Jon, Brian

Dishes - Brian

Filter LTER leachate, filter LTER soil extracts - Sam, Jon, Emily, Glori

Prep LTAR gas vials - Olivia, Brian

Prep LTER gas vials, totes - Stacey

Prepared 1M KCL.

Weigh LTER soil for extraction and moisture - Sam, Jon

WPS Zoom - Sam

Dishes - Jon

Grind SBE 2025 plants -

Sieve LTER soil - Brian, Jon, Emily, Sam

Dishes, vial prep - Olivia

Safety training - Bella

Finish sorting ASP2 plants - Brian, Olivia, Sam

Dishes - Jon

Prep gas vials for fertilization side project with Kevin, looking at gas fluxes over knifed-in vs. surface applied nitrogen - Olivia

Measure and filter LTAR leachate - Brian, Jon, Emily

Prep BCSE gas vials, totes - Sam, Emily

Prep LTAR gas vials - Stacey

Onboarding - Sam

Dishes - Brian

Sort ASP2 plants - Alyssa, Jon

Dishes - Jon

Sort ASP2 plants - Emily, Stacey

Sort ASP2 plant samples - Emily, Brian, Jon

Filter LTER leachate - Jon

Filter BCSE, LTAR soil extracts, dishes - Stacey, Brian, Emily, Sarah

Sequence LTAR20260423S6BGC had two samples switched in trays submitted from field techs. Samples were all run in sequential order on the GC, but in trays, samples 83 and 84 were swapped from their correct positions.

Prepared 1M KCL.

Weigh BCSE 4/24/2026 and LTAR April soil for extraction and moisture - Brian, Olivia