Projectlog Entries List

Finish sieving and subsampling GLBRC SVII soil. Finish relocating soil archive for construction. - Emily, Jon, Glori

Weigh LTAR plot R3,4 0-10cm soil into 50mL centrifuge tubes for microbial CN. - Jon, Stacey

Sieve GLBRC SVII soil and subsample for A&L. - Jon, Glori, Emily

Extract and filter LTAR R3,4 unfumigated soil for microbial CN. Add chloroform to fumigated soils. Dishes - Stacey

LTAR gas vial prep - Alyssa, Stacey

Weigh LTAR plot R1,2 0-10cm soil into 50mL centrifuge tubes for microbial CN. Dishes - Jordyn, Stacey

Sieve LTAR R3,4 0-10cm soil from Sprunger lab. Sieve SBE soil and subsample for A&L. - Jon, Glori, Jordyn

Extract and filter LTAR R1,2 unfumigated soil for microbial CN - Stacey

Dishes - Jon

Weigh LTAR and MLE Lux Arbor dry soil tins - Alyssa

Sieve LTAR R1,2 0-10cm soil from Sprunger lab. Continue to relocate soil archive samples. - Glori, Emily, Jon

Temporarily relocate soil archive from the field lab to P.B. wash bay during field lab construction - Jon, Emily, Glori, Jordyn, Alyssa, Stacey

Dishes - Jon

Filter MLE Lux Arbor soil extracts - Jon, Glori

Weigh MLE Lux Arbor soil for extraction and moisture - Emily, Glori

Prepared 1M KCL.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Acid washed dishes.

Dishes - Jon, Glori

Sieve MLE Lux Arbor soil - Emily, Glori, Jordyn

Filter LTAR soil extracts, dishes, weigh dry MLE Lake City soil tins - Jon, Glori, Jordyn, Emily

Finish sieving LTAR plot soil - Alyssa, Emily

Weigh LTAR soil for extraction and moisture - Jon, Glori

Weigh dry MLE Escanaba soil tins - Stacey

LTAR gas vial, tote prep -

Sieve LTAR soil, remove subsamples for A&L S1 analysis from main plot soil only (not noNI microplots) - Alyssa, Emily, Jon, Glori

Filter MLE Lake City soil extracts - Jon, Glori

Weigh MLE Lake City soil for extraction and moisture - Jordyn, Glori

Filter MLE Escanaba soil extracts - Emily, Jon

Prepared 1M KCL.

Prepared 1M KCL

Weigh MLE Escanaba soil for extraction and moisture -

Sieve MLE MN Escanaba (sampled 10/13/2026) and MC Lake City (sampled 10/14/2026) soil samples - Alyssa, Glori, Emily, Jon

LTER field lab clean-up - Stacey

BCSE gas vial, tote prep - Glori

Filter MLE Rhinelander soil extracts - Glori, Alyssa

Weigh MLE Rhinelander soil for extraction and moisture - Alyssa, Jordyn

Filter MLE Hancock soil extracts - Jon, Emily

Prepared 1M KCL.

Weigh MLE Hancock soil for extraction and moisture , dishes - Jon, Emily, Glori

Prepared 1M KCL.

Finish MLE Rhinelander soil sieving - Alyssa, Olivia

Dishes - Alyssa

NAS data entry - Olivia

Sieve Hancock (10/6/2026) and Rhinelander (10/7/2026) soil. Sampled by Chris and Todd. - Emily, Glori, Alyssa

Weigh dry soil tins, dishes - Stacey

LTAR gas vial, tote prep. Filter LTER soil extracts. Dishes - Olivia, Glori

Weigh LTER soil for extraction and moisture - Glori, Olivia

Prepared 1M KCL

Sieve LTER October soil. Remove 200g subsamples for Yang Lin from T1,2,4 R1,2,4,5. - Alyssa, Emily, Glori, Olivia

Measure and filter LTAR leachate - Olivia, Emily

Pack LTAR 2024 plant CN - Jordyn, Olivia

Filter LTER T3, T4 soil extracts, dishes - Alyssa, Emily, Glori, Jordyn

Prepared 1M KCL

Weigh T3, T4 station and prairie strip soil for extraction and moisture - Olivia, Glori, Alyssa

Finish sorting ASP1 forage - Emily, Glori

Pack LTAR 2024 plant CN - Stacey, Alyssa

Sieve LTER T3, T4 station and prairie strip soil. Sort ASP1 forage - Alyssa, Olivia, Emily, Glori

Acid washed dishes

Weigh G2 sorghum ANPP samples before putting into the oven - Glori, Emily, Jordyn, Olivia, Alyssa

Lab clean-up, update ChemInventory - Alyssa, Stacey

Weigh dry LTAR soil tins - Stacey

DawnGC run notes: BCSE Series 16 - vial 12 flipped upside down in sample tray. Guessing this means there was no sample taken, but vial flipped upright and analyzed to maintain continuity throughout the runs.

DawnGC run notes: LTER Series 12 - duplicate 3 sample vial was empty and vial 84 used a spare vial in its stead. All samples run sequentially on the GC with the spare vial replacing vial 84 and duplicate 3 kept in line as is.

Safety training - Alyssa

ChemInventory updating - Alyssa, Stacey

Dishes - Olivia

DawnGC run notes: NAS Series14, sampled 07/21/2026 - one set of four was submitted out of order as follows, Vial 17, 20, 19, 18. Vials all run sequentially on the GC, so these may need to be resorted, depending on field note references.

DawnGC run notes: LTAR Series 23, sampled 9/11/2026 - submitted from field with these notes - "vials 50 & 59 swapped, vial 129 broken." Samples all run on GC sequentially, and a dummy vial placed in the stead of vial 129.

Sort ASP1 forage - Olivia

Sort ASP1 forage - Olivia

Filter LTAR extracts, dishes - Jon, Glori

LTAR, LTER gas vial and tote prep - Stacey

Prepared 1M KCL

Weigh LTAR soil for extraction and moisture - Jon, Emily

Filter LTER leachate, dishes - Olivia, Glori

Sieve LTAR soil - Alyssa, Olivia

Weigh dry LTER soil moisture tins, start checking GLBRC transect data (Sven scanned) - Stacey

Filter LTER soil extracts, dishes - Jordyn

Weigh LTER September soil for extraction and moisture - Stacey

Sieve LTER soil - Stacey

Sieve LTER soil - Emily, Glori, Olivia

LTAR, BCSE gas vial and tote pre - Olivia, Jordyn, Stacey

Finish sort SF1 ANPP, field lab clean-up - Alyssa, Jon, Emily, Glori, Olivia

Sort SF1 ANPP - Emily, Jon, Glori, Olivia, Alyssa

Sort SF2, SF3 ANPP - Stacey

Sort SF1 ANPP - Olivia, Jordyn

Sort SF1 ANPP - Alyssa, Jon, Emily, Jordyn, Glori, Olivia

Pack LTAR 2024 plants and LTER 2024 RR CN - Bella, Jordyn

Weigh LTER 2026 plants - Olivia

DawnGC sequence LTAR-S21 had a few samples submitted out of order in field trays. Order submitted as follows: ....82, 83, 88, 85, 86, 87, 84, 89, 90,.... All samples run on DawnGC in sequential vial order.

Sort T6 ANPP - Alyssa

Pack LTAR 2024 plants CN - Olivia

BCSE S13 S14 S15 GC run had some samples submitted out of order.
S14 had vials 19 and 20 switched in the field racks. S15 had four out of order submitted as follows: vial 52, 50, 51, 49 All samples run sequentially on DawnGC

LTAR gas vial, tote prep -

Finish sorting T7 ANPP - Olivia, Jordyn, Glori, Alyssa, Jon, Stacey

Sort T7 ANPP - Alyssa, Glori, Bella, Jordyn, Emily, Jon, Olivia

Pack CN - Jon

Sort T7 ANPP - Alyssa, Glori, Bella, Stacey

Data entry - Glori

LTER gas vial, tote prep - Stacey, Jordyn

Weigh LTER plants - Sam pm

Data entry - Jon pm

Pack CN - Bella pm

GC run on Dawn GC: LTAR, Series 19, 07/23/2026. Two vials submitted out of order - 40 before 39. These were switched to proper order on the GC rack and all vials run sequentially. In addition, there was an issue with LTAR Series 18 being stuck on "acquiring" at the very last standard in it's sequence. This caused an issue with loading the Series 19 run, until the Series 18 was aborted for the last standard, and the run cleared from the Run Queue. I think everything worked out, but there may be no data for that last standard on series 18.

LTER Series 8 06/26/2026 GC run had some samples submitted out of order. All samples run in sequential order, but these vials submitted out of order (as listed): ...220, 224, 222, 223, 221, 225....

LTAR Series 18, 07/20/2026 GC run. Two samples submitted out of order. All samples run sequentially. Sample vials 80 and 79 were swapped in racks submitted for analysis (i.e. vials ordered ...76, 77, 78, 80, 79, 81....).

Sort LTER T3/T4 Prairie Strip ANPP plants - Alyssa, Glori, Jordyn AM/PM

Dishes - Sam AM, Jon PM

Sort T3/T4 Prairie Strip ANPP - Alyssa, Jon, Glori, Bella, AM, Alyssa, Sam, PM

Dishes - Sam, Jon

Sort T3, T4 prairie strip ANPP plants - Sam, Jon, Emily

Sort T3, T4 prairie strip ANPP plants - Alyssa, Olivia, Jon

Sort T3, T4 prairie strip ANPP plants - Alyssa, Glori, Stacey

LTER gas tote prep - Stacey

WPS - Emily

Sort T3, T4 prairie strip ANPP plants - Alyssa, Brian, Olivia

Filter T3, T4 T21 soil extracts - Glori, Jon, Emily

LTER gas vial prep - Stacey

Weigh T21 soil from T3, T4 stations and strips - Brian, Bella, Glori

Prepared 1M KCL

Finish pooling T21 soil - Alyssa, Glori, Emily

Sort T3, T4 R1 prairie strip ANPP - Sam, Jon, Brian, Emily, Alyssa, Glori, Stacey

BCSE gas vial and tote prep - Stacey

Pool T21 soil bags A and B from T3, T4 stations and prairie strips -

LTAR gas vial and tote prep - Sam, Jon

Measure then filter LTAR leachate, filter LTAR soil extracts, dishes - Glori, Bella, Sam

Sort ASP6 ANPP plants - Stacey, Alyssa, Jon, Emily

Acid washed dishes.

Weigh LTAR soil for extraction and moisture -

Sort ASP6 ANPP plant samples - Stacey, Jon, Sam, Emily, Brian, Bella, Glori, Jordyn

Prepared 1M KCL, prepared standards. Acid washed dishes. Lachat run 2026 July LTER T7 soil (Lau lab).

Lachat runs GLBRC soil 20260424 and 20260622.

Sieve LTAR soil samples -

Sort ASP6 ANPP -

Sort T8 ANPP - Alyssa, Stacey, Jon

Filter LTER T21 soil extracts - Brian, Jordyn

Sort T8 ANPP plants - Alyssa, Stacey, Jordyn, Jon, Olivia, Bella, Glori, Sam

Weigh LTER T21 soil samples for moisture and extraction - Brian, Bella

LTER gas vial and tote prep. WPS training - Emily

Prepared 1M KCl

Measure and filter LTER leachate, dishes - Jon, Jordyn

Start sorting T8 ANPP -

Sort T5 ANPP - Brian, Emily, Jordyn, Olivia, Stacey

Lachat run LTER Lysimeter 20260402. Notes from run:

• Three vials labeled T3 R3 1, 2, and 3 were ran as T5 R3 1, 2, and 3. Flasks for T3 R3 and T5 R3 were swapped when evacuating samples. Weights were adjusted when entering in data file to the correct location, but vials were sampled incorrectly.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

LTER Grad Fellow Symposium

Pack CN - Glori

Data entry - Emily, Jon

LTER field lab to do list (cleaning, organization, dump old samples) - Jordyn, Sam, Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Weigh LTAR plants - Jon, Jordyn

Sort ASP1 forage - Emily, Brian, Bella, Jon, Sam, Olivia

Pack CN - Sam, Bella

Dishes, lab clean-up - Jordyn, Glori

NAS vial prep and data entry - Olivia

Lachat run LTER Lysimeter 20260319. Notes from run:

• Vial labeled T2 R4 1 should not exist, not enough volume listed. Ran as Vial T3 R4 1, that was not present and should exist.
• Vial T CF R2 1 missing from box, not run (no data notes)
• Vial T6 R3 3 present, should not exist. Not enough volume in vial to run.

Replaced Cadmium Column, acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopore H2O.

Prepared Standards.

Lachat run LTAR Lysimeter 20260616. Notes from run:

• Vial labeled ASP2 R1 N should not exist, not enough volume. Ran as vial ASP2 R2 N, that should exist and is missing.
• Vial ASP6 R2 S was missing from box and should exist, not run.

Measure and filter LTAR leachate, filter LTER soil extracts, LTAR and BCSE gas vial prep - Jordyn, Glori, Emily, Brian

Sort ASP1 forage - Sam, Jon

Sieve LTER soil -

Weigh LTER soil for extraction and moisture - Alyssa,

Sort ASP1 forage, empty ovens - Alyssa

Prepared 1M KCl

Make new LTER gas vials; all previous vials are awaiting analysis due to troubles with the old GC and switch over to a new GC - Alyssa, Olivia