Grind LTER plants - Olivia, Brian

LTER gas vial and tote prep - Brian

T7 microplot plant archive data entry - Jon

Grind BCSE plants on campus - Jon, Brian

Grind LTER plants - Sarah

Grind BCSE plants on campus - Alyssa, Jon

Grind LTER plants - Brian, Olivia

BAF subsampling - Slater

Pack CN - Slater, Brian

Weigh SBE plants, grind LTER plants - Slater, Brian

T7 microplot plant archive data entry - Jon

Pack CN - Olivia

Weigh SBE plants, grind LTER plants - Sarah, Brian, Slater

T7 microplot plant archive - Jon

Pack CN - Slater, Brian

Weigh SBE plants - Slater, Jon

Grind LTER plants - Jon, Slater

Pack CN - Olivia

Reconditioned Cadmium Column, Acid washed dishes.

Lachat run LTER T4 soil 20250527

Weigh SBE plants - Brian

Grind LTER plants - Slater, Sarah

T7 microplot plant archive - Jon

Data entry, BAF subsampling - Sarah, Slater

Grind LTER and weigh SBE plants - Brian

Weigh plants - Brian

Grind plants - Olivia, Slater

Continue checking T7 microplot plant archive - Jon

Pack CN - Slater

Grind and weigh plants - Slater, Sarah, Brian

Check LTER T7 microplot plant archive jars going back to the start of the project against a spreadsheet created from ANPP data to establish a T7 microplot plant archive data set similar to what we already have for the LTER main plots. - Jon

Pack CN - Slater, Sarah

Grind plants - Slater, Jon

Weigh plants - Brian

Data entry - Olivia

Pack CN - Jon, Slater

Grind plants - Brian, Slater

Finish SWF BAF subsampling - Brian

Weigh plants - Jon

Data entry - Olivia

Pack CN - Alyssa

Main lab and field lab clean-up -

Weigh plants - Alyssa, Brian

Grind plants - Jon, Alyssa

LTAR gas vial, tote prep - Olivia

BAF subsampling SWF soil - Brian, Slater

Data entry - Slater, Jon

Grind plants - Alyssa, Slater

Pulverize SWF deep core soil - Slater, Alyssa

Start subsampling SWF soil for BAF - Alyssa

Data entry - Brian

Weigh plants - Sarah

GC run LTER20250112S17BGC had a vial set shift on one sheet. This was recorded on the sheet and at data entry. All vials run in sequential order on the GC. This is just for reference in case you want to verify that results line up with sites (but again, this should have been fixed on tech data entry of sampling forms): vial set 5,6,7,8 shifted to slots 9,10,11,12; vial set 9,10,11,12 shifted to slots 17,18,19,20; and, vial set 17,18,19,20 shifted to slots 5,6,7,8.

Grind plants - Brian, Slater

Pulverize SWF deep core soil - Slater, Alyssa

Data entry - Alyssa, Brian

Pack CN - Olivia

Data entry - Slater, Brian, Sarah

Weigh and grind plants - Alyssa, Olivia, Brian

Weigh plants - Olivia, Alyssa

Finish BAF subsampling, blow out Torit filters, grind plants - Slater

Pack CN - Brian

BAF BCSE plants subsampling - Olivia

Weigh plants - Slater

Data entry, WPS training - Alyssa

Weigh and grind plants - Sarah, Slater

Pack CN - Olivia

Acid washed dishes.

Weigh and grind plants - Brian, Olivia

Finish weigh roots, BAF subsampling - Jon

Pack CN - Slater, Jon

Weigh and grind plants - Sarah, Jon

Pulverize SWF soil -

Weigh roots, pack CN - Slater, Olivia

Weigh plants - Brian

Grind plants, pulverize SWF 2024 deep core soil - Slater, Olivia

Weigh 2024 Switchgrass Nitrogen Rate Experiment deep core roots - Jon

Weigh plants - Brian, Slater

Grind plants - Jon, Olivia

BAF subsampling - Olivia

Weigh 2023 Switchgrass Biodiversity Experiment deep core roots - Brian, Jon

Pack CN - Slater

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Acid washed dishes.

Pack CN - Stacey, Olivia, Brian

Weigh plants - Brian, Olivia

Grind plants - Slater

BAF subsampling (BCSE harvest 2021) - Sarah

Weigh 2023 Switchgrass Biodiversity Experiment deep core roots - Jon

Pack CN - Stacey, Slater

Weigh plants - Jon

Grind plants - Olivia

LTAR gas vial, tote prep - Slater

BAF subsampling (BCSE harvest 2020) - Brian

Pack CN - Brian, Olivia

Weigh plants - Olivia, Brian

Grind plants - Sarah, Slater

BAF subsampling (BCSE harvest 2019) - Slater, Jon

BCSE gas vial, tote prep - Jon

Dishes -

Pack CN - Slater, Brian

Weigh plants - Brian, Olivia

Grind plants - Slater

Lab clean-up, dump soil buckets, weigh dry tins - Sarah

LTAR gas data entry - Jon, Olivia

Archive LTAR air-dried soil -

Filter LTAR leachate; collection included many more samples than 11/24-25, so those archive samples were discarded and this collection was archived in it’s place. Also, subsamples from ASP3 and BAU2 leachate were delivered to Younsuk Dong and Subhasis Giri on campus by Dave Weed for phosphorus analysis at Brook’s request. - Slater, Sarah

Filter LTAR soil extracts - Slater, Sarah

Finish sieving LTAR soil - Jon, Alyssa

Weigh LTAR soil for extraction and moisture - Jon, Alyssa, Brian, Sarah

LTER gas vial/tote prep, weigh dry soil tins, dishes - Slater, Brian, Jon, Sarah

Start sieving LTAR soil - Alyssa, Slater, Brian, Jon, Sarah

Dishes, LTAR gas data - Slater, Brian, Olivia, Jon

Filter BCSE subplot soil extracts - Alyssa, Brian, Olivia

Weigh BCSE subplot soil for extraction and moisture, plus subsample G1-3 main and subplot to send to A&L - Slater, Jon

LTAR gas data - Brian, Jon

Dishes - Brian, Slater

Sieve BCSE subplot soil -

Filter BCSE main soil extracts, dishes -

Finish sieving BCSE main soil - Jon, Brian

Weigh BCSE main soil for extraction and moisture - Jon, Brian, Slater

Filter MLE MC soil extracts - Jon, Slater, Brian

Prepared 1M KCL, standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

GC sequence LTAR20251126S29BGC had two issues with vial order submitted in trays. Vials 100 and 101 were switched in trays, and vials 135 and 136 were switched in trays. All GC vials were run sequentially on instrument.

Weigh MLE MC soil (sampled 11/25/2025) for extraction and moisture - Brian, Jon

Sieve BCSE main soil - Alyssa, Sarah, Olivia, Slater, Jon, Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared 1M KCL.

Sequence BCSE20251120S22BGC had a couple vial submission issues.
Duplicate 1 was swapped with Sample 1 in field tray. Vials 5 and 6 were swapped (out of order) in tray. Samples run in this order: Duplicate 1, 2, 3, 4, 5, 6,… …., 79, 80, Air1, Air2, Air3, Air4, 1, Dup2, Dup3, Dup4.

Sieve MLE MC Lake City soil, sampled 11/25/2025; dishes, weigh dry soil tins - Brian, Jon, Slater, Sarah

Sample BCSE soil respiration jars - Sarah

LTAR gas vial, tote prep -

Filter MLE WC Hancock soil extracts, dishes -

Measure, and filter LTAR leachate, dishes -

Weigh MLE WC Hancock soil for extraction and moisture - Brian, Sarah

Dishes - Brian, Sarah

Sample BCSE soil respiration jars - Sarah

Sample BCSE soil respiration jars - Sarah

Sieve MLE WC - Hancock soil, sampled 11/19/2025 - Jon

Filter LTER leachate - Alyssa

Filter WN soil extracts - Olivia, Brian

Set up and sample BCSE soil respiration jars with Kevin - Sarah

Prepared 1M KCL. Finished dilutions from LTAR Lysimeter 20250610.

Sieve MLE WN - Rhinelander soil, sampled 11/18/2025. Weigh dry soil tins - Stacey

Weigh WN soil for extraction and moisture - Stacey, Alyssa

BCSE gas vial, tote prep - Brian

Filter LTER soil extracts - Jon, Brian

Weigh LTER soil for moisture and nitrogen extraction - Brian, Jon

Pack CN - Brian

Dishes - Jon

Prepared 1M KCL, Lachat run LTAR Lysimeter 20250529.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Sieve LTER soil - Alyssa, Brian, Olivia, Jon

GC Sequence LTAR20251113S28BGC had a few vials submitted out of order. All samples run sequentially on GC. Vials submitted out of order as follows: …62, 63, 68, 65, 66, 67, 64, 69, 70… Also, note on tape says vials 41 and 44 were swapped, though they were submitted in sequential order in racks.

Sieved BCSE soil and set out to air-dry - Sarah

Sieved Sorghum Nitrogen Rate soil and subsampled to send to A&L for S1 analysis. - Slater, Jon

Filter LTAR leachate, dishes -

Measure LTAR leachate - Slater, Brian

LTAR gas vial, tote prep - Olivia, Sarah

Weigh dry tins, dishes - Sarah

Put away clean, dry dishes, weigh remaining BCSE grain, grind SWF 2024 harvest plant samples - Jon

Finish sorting ASP2 plants - Sarah

Sort ASP2 plants, put LTAR 11/3/2025 soil out to air-dry - Slater, Brian, Jon

Finished dilutions from Lachat run LTAR Lysimeter 20250318.

Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTAR Lysimeter 20250401. Notes from run:

• Vials BAU1 R2 S and BAU1 R4 S missing from box, not run.
• All bottles were collected after 48 hours instead of 24 due to storms.

Sort ASP2 plants - Slater, Brian, Olivia

Finish BAF subsampling for 2024 BCSE peak plant CN - Sarah

Dishes - Jon

Filter LTAR extracts, dishes - Slater, Brian, Jon

Prepared 1M KCL.

Weigh LTAR soil for extraction and moisture - Alyssa, Jon

Prep LTER gas vials, totes - Alyssa, Jon, Brian

Sort ASP2 plants - Alyssa, Slater, Brian, Jon

Sieve and subsample LTAR soil for A&L - Jon, Brian, Olivia, Sarah

Weigh dry soil tins, dishes - Slater

BCSE20251023S21BGC sequence notes: All samples run in sequential vial number order. Samples submitted out of order in trays as follows: …59, 60, 61, 78, 79, 64, 65, 74, 75, 68, 69, 70, 71, 72, 73, 66, 67, 76, 77, 62, 63, 80…

Sieve SWF soil and put a portion in A&L soil bags to send for agronomic analysis. - Slater, Jon, Brian, Emily

Sort ASP1 plants - Ainsley, Olivia

Weigh plants - Brian

Grind plants - Slater

Sort ASP1 plants - Alyssa, Ainsley, Brian, Emily, Jon, Slater

LTAR gas vial, tote prep - Ainsley, Slater

Weigh plants - Brian

Filter MLE MN soil extracts - Ainsley, Jon

Weigh MLE MN soil for extraction and moisture - Ainsley, Brian

Measure and filter LTER leachate - Alyssa, Emily

Pack CN - Ainsley

BAF subsampling for BCSE 2024 - Jon

Grind plants - Slater

Weigh plants - Brian

Dishes, weigh dry soil tins - Slater, Ainsley

Weigh plants - Sarah

Grind plants - Jon

Sieve MLE MN soil (sampled 10/23/2025) - Ainsley, Slater, Emily

Weigh plants - Jon, Ainsley

Pack CN - Ainsley, Slater

Weigh plants - Slater

Pack CN - Ainsley, Jon

Filter MLE MS soil extracts - Emily, Ainsley

Prepared 1M KCL

Measure and filter LTAR leachate - Slater, Jon

BCSE gas vial, tote prep - Sarah

Weigh MLE MS soil for extraction and moisture - Ainsley, Emily

Weigh plants - Olivia, Ainsley

Pack CN - Slater

Data entry -

LTER20251010S14BGC samples submitted out of sequence. All sample vials run in numerical order, but they were submitted in this order: …161, 162, 167, 164, 165, 166, 163, 168, 169, 170… then again, …219, 220, 222, 221, 223, 224, 225…

LTAR20251016S26BGC GC sequence run had sample vials submitted out of order. All sample vials run on GC in numerical order. Submitted vials out of order as follows: …96, 97, 118, 119, 120, 101, 122, 123-144, 117, 98, 99, 100, 121, 102, 103-116.

Weigh plants - Alyssa, Ainsley

Pack CN - Sarah, Alyssa

Sieve MLE MS soil (sampled 10/21 am by Todd and Chris) - Slater, Emily, Jon

Pack CN - Olivia, Jon

Weigh plants - Slater, Ainsley

Dishes - Slater

ChemInventory spreadsheet set up - Alyssa

Finish checking transect data - Slater

Weigh plants - Emily, Alyssa

Pack CN - Alyssa, Brian

Filter LTER soil extracts - Ainsley, Emily

Weigh LTER soil for extraction and moisture - Olivia, Ainsley

Weigh plants - Brian

Transect data entry/checking - Ainsley, Alyssa

Pack CN - Slater, Stacey

Prepared 1M KCL

LTAR gas vial, tote prep - Emily, Sarah

Weigh plants - Ainsley

Transect data entry/checking - Emily, Sarah, Alyssa, Olivia

Pack CN - Sarah, Stacey

Weigh dry soil tins, dishes - Brian

Sieve LTER soil - Slater, Emily, Jon

Transect data entry/checking - Brian, Ainsley, Emily, Slater

Weigh G1 corn - Alyssa

Dishes - Brian

Pack CN - Stacey

Filter LTAR soil extracts, dishes - Slater, Emily

Pack CN - Stacey

Weigh LTAR soil for extraction and moisture - Brian, Ainsley

LTER gas vial and tote prep - Slater

Transect data entry - Jon

Pack CN - Stacey

Transect data entry - Brian, Ainsley

Weigh plants - Sarah

Pack CN - Stacey

Pack CN - Stacey

Weigh plants - Brian, Jon

Plant species composition transect data entry - Emily, Slater, Ainsley, Brian

Weigh dry soil tins, dishes - Jon

Plant species composition transect data entry 2025 - Olivia, Brian

Dishes - Sarah

Weigh plants - Ainsley, Alyssa

Transect data entry - Ainsley

Transect data entry - Alyssa

Measure and filter LTAR leachate - Brian, Emily

Pack CN (LTER 2020 plants) - Stacey

Transect data entry - Olivia, Ainsley, Brian, Jon

LTAR gas vial and tote prep - Brian, Sarah

Filter LTER T3, T4 soil extracts - Slater, Sarah, Emily

Pack CN (LTER 2020 plants) - Stacey

Weigh T3, T4 soil for extraction and moisture - Brian, Ainsley, Jon

Measure and filter LTER leachate, dishes - Alyssa, Emily, Jon

Plant species composition transect data entry - Slater, Ainsley, Brian

Prepared 1M KCL

Sieve LTER T3, T4 soil samples -

Plant species composition transect data entry -

Plant species composition transect data entry - Brian

GC Sequence LTAR20250918S24BGC had vials submitted out of order. All vials run in sequential order, but these two vials were out of order as submitted: 34 and 33.