Projectlog Entries List

GC Sequence LTER20260401S2BGC had some vials submitted out of order in the field trays. All samples were run in sequential order on the GC. Vials submitted out of order were as follows: …148, 150, 149, 151, 152… and …241, 242 ,247, 244, 245, 250, 251, 248, 249, 246, 243, 252, 253…

GC Sequence LTAR202603250402S2S3BGC had one set of sample vials out of order, as submitted by Techs. Vials were in no discernable order for that last rack so recorded in this order: 189,190,191,172,169,186,187,176,173,182,183,180,177,178,179,184,181,174,175,188,185,170, 171,192. Could be read in the opposite order as well? Anyhow, all samples run on GC in sequential order. Will look to see if this rack simply got jumbled up in the transport from field to lab.

Acid washed dishes.

Lachat run LTER Lysimeter 20250421. Run notes:

• Samples T1 R2-2 and T7 R2-1 should have samples, but no vial was in sampled box, skipped.
• Sample CF1-1 has no vial, should exist. Vial labeled as CF1-6 does not have enough volume listed to filter, ran as CF1-1.

Filter LTAR soil extracts - Jon

Weigh LTAR soil for extraction and moisture - Brian, Olivia

Prep LTER, LTAR gas vials - Emily

Prepared 1M KCL, Lachat run LTER Lysimeter 20250408.

Replaced end filter and 4th cartridge filter on Barnstead nanopure. Ran water test on Lachat, filter seems to be functioning normally again.

Sieve LTAR soil - Alyssa, Brian, Jon, Emily

Measure and filter LTAR leachate; dishes - Brian, Olivia

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Finished GLBRC Microplot soil 20251203 Lachat run and Re-extracted GLBRC MLE MC (Lake City) soil 20251125.

Filter LTER soil extracts - Brian, Sarah

Weigh LTER soil for extraction and moisture - Olivia, Brian

LTAR gas vial prep - Jon

Dishes - Brian, Jon

Prepared 1m KCL. Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Sieve March LTER soil - Alyssa, Sarah, Brian, Jon, Stacey

Measure and filter LTER leachate - Jon, Brian

Grind SBE plants - Olivia

Grind SBE plants - Brian

Pack LTAR 2023 plants - Jon

Dishes, lab cleaning - Sarah

Grind SBE plants - Jon

Pack LTAR 2023 plants - Brian

Safety training - Brian, Jon, Sarah

Fall project wrap-up - Sarah

Lab cleaning - Alyssa, Stacey

GC Sequence LTAR20260312S1BGC had a few samples submitted out of order. All samples run in numerical sequence on GC. Samples Dup3 and Dup4 were swapped with vials 3 and 4 as submitted. They were swapped back in proper order on GC run. Another sample out of order towards the end as such: ….112, 116, 113, 114, 115, 117, 118….

Pack LTAR 2023 CN - Olivia

Dishes - Jon, Olivia

Grind SBE 2025 - Brian

Relabel 2019, 2021 BCSE G2 harvest archive jars - Jon

Inventory LTER leachate archive - Brian

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Dishes, grind SBE palnts, inventory LTER leachate samples - Sarah, Jon, Brian

Grind SBE plants - Olivia, Brian

Measure and filter LTAR leachate, save archive sample, dishes - Olivia, Jon

Grind SWF plants - Sarah, Jon

Grind LTAR 2025 plants - Sarah, Jon

One last check on LTER T7 microplot plant archive samples where ANPP records don’t match Archive records - Jon

Pack LTAR 2023 plant CN - Olivia

Grind LTAR 2025 plants - Sarah

Pack LTAR 2023 plant CN - Jon

Prep LTAR gas vials - Olivia, Alyssa

GC sequence BCSE20260303S1BGC notes: All samples run in sequential order. One set submitted out of order in trays as follows: vial # ….44, 45, 47, 46, 48, 49….

Grind LTAR plants - Sarah

Acid washed dishes, Replaced Cadmium column.

Prep BCSE gas vials and totes - Jon

Prepared standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Grind LTAR 2025 plants - Brian, Jon

Pack LTAR 2023 plant CN - Jon

Grind LTAR 2025 plants - Olivia

Pack LTAR 2023 plant CN - Brian

Relabel BCSE 2021 archive jars - Jon

Filter 8/20 soil extracts, dishes - Jon, Brian

Grind LTAR 2025 plants - Jon

Reweigh LTER 8/20/2025 soil samples for extraction and moisture, initial results were good but reextracted to compare to other ammonium contaminated samples. Also, some of this soil warmed up when a walk-in stopped working, so interesting to compare initial to warmed samples. - Brian, Sarah

Relabel BCSE 2021 archive jars - Brian

Weigh dry soil tins, wash dishes - Sarah

Prepared 1m KCL

Lachat run LTER soil 20250806 (Re-extracted on 2/23/26 to compare to contaminated samples)

Grind LTAR 2025 plants - Brian

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Jon

Filter 8/6 soil extracts - Jon, Brian

Grind LTAR 2025 plants - Jon, Sarah

Reweigh LTER 8/6/2025 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts, results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Brian, Sarah

Relabel BCSE 2019, 2021 archive jars - Jon

Weigh dry soil tins, wash dishes - Brian

Prepared 1M KCL

Lachat run LTER T3 soil 20250723 (Re-extracted on 2/18/2026 due to KCl contamination)

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Brian

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Brian

Filter 7/23/2025 soil extracts - Jon, Brian

Grind LTAR 2025 plants - Sarah

Reweigh LTER 7/23/2025 T3, T4 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts; results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Jon, Brian

Lachat run LTER T4 soil 20250929.

Prepared 1M KCL.

Finish grinding BCSE 2025 plants, start grinding LTAR 2025 plants - Olivia, Brian

Pack LTAR 2023 plant CN - Jon

Cleaning at field lab and trucks, start relabeling BCSE 2019 plant archive jars with labels Nick Thrower sent with vertical barcodes so scanner can read them (they were horizontal and went around the corner of the jars, so couldn’t be read by a scanner) - Brian

Grind BCSE plants - Jon

Pack LTAR plant 2023 CN - Brian

Finish subsampling SWF plants for BAF, cleaning - Sarah

Acid washed dishes. Lachat run LTER Soil 20250915.

Grind BCSE plants - Brian

Finish packing LTER plant 2023 CN, start packing LTAR 2023 plants - Jon, Olivia

GC Sequence BCSE20260205S25BGC vials submitted out of sequence by techs. All vials run in numerical sequence on GC. Vials submitted out of sequence as follows: …31,32,34,33,35,36… …50,51,56,53,54,55,52,57,58….

GC Sequence LTER20260206S18BGC notes. Vials 13-16 were not sampled (techs couldn’t find chamber in the snow), but run on GC as empty vials to keep run in order. Tech notes that vials 73-112 contained swapped sets (i.e. vial 93-96 were used for sheet slots 73-76). These were corrected on the field note sheets and entered at the field note data entry to properly line up the vials with the sites

Grind BCSE plants - Jon

Pack LTER plant 2023 CN - Brian

Grind BCSE plants - Brian

Pack LTER plant 2023 CN - Jon

Grind ASP6 POACEAE (combined grasses) on campus, deliver BCSE grain harvest samples to Chris - Jon, Brian

Grind BCSE plants - Olivia

Grind BCSE plants - Sarah

Pack CN - Brian

Sort ASP6 samples to grind on campus 2/10/2026 - Jon

Finish grinding LTER plants -

Prepare LTAR gas vials and totes -

Grind BCSE plants on campus - Brian, Sarah

Prepare BCSE gas vials and totes - Alyssa

Finish entering T7 microplot plant archive data - Jon

Acid washed dishes, prepared Standards.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER T4 soil 20250723. Contamination in KCL blanks in NH4 results, not uploaded.

Grind LTER plants - Olivia, Brian

Prepare LTER gas vials and totes - Brian

T7 microplot plant archive data entry - Jon

Grind BCSE plants on campus - Jon, Brian

Grind LTER plants - Sarah

Grind BCSE plants on campus - Alyssa, Jon

Grind LTER plants - Brian, Olivia

BAF subsampling - Slater

Pack CN - Slater, Brian

Weigh SBE plants, grind LTER plants - Slater, Brian

T7 microplot plant archive data entry - Jon

Pack CN - Olivia

Weigh SBE plants, grind LTER plants - Sarah, Brian, Slater

T7 microplot plant archive - Jon

Pack CN - Slater, Brian

Weigh SBE plants - Slater, Jon

Grind LTER plants - Jon, Slater

Pack CN - Olivia

Reconditioned Cadmium Column, Acid washed dishes.

Lachat run LTER T4 soil 20250527

Weigh SBE plants - Brian

Grind LTER plants - Slater, Sarah

T7 microplot plant archive - Jon

Data entry, BAF subsampling - Sarah, Slater

Grind LTER and weigh SBE plants - Brian

Weigh plants - Brian

Grind plants - Olivia, Slater

Continue checking T7 microplot plant archive - Jon

Pack CN - Slater

Grind and weigh plants - Slater, Sarah, Brian

Check LTER T7 microplot plant archive jars going back to the start of the project against a spreadsheet created from ANPP data to establish a T7 microplot plant archive data set similar to what we already have for the LTER main plots. - Jon

Pack CN - Slater, Sarah

Grind plants - Slater, Jon

Weigh plants - Brian

Data entry - Olivia

Pack CN - Jon, Slater

Grind plants - Brian, Slater

Finish SWF BAF subsampling - Brian

Weigh plants - Jon

Data entry - Olivia

Pack CN - Alyssa

Main lab and field lab clean-up -

Weigh plants - Alyssa, Brian

Grind plants - Jon, Alyssa

LTAR gas vial, tote prep - Olivia

BAF subsampling SWF soil - Brian, Slater

Data entry - Slater, Jon

Grind plants - Alyssa, Slater

Pulverize SWF deep core soil - Slater, Alyssa

Start subsampling SWF soil for BAF - Alyssa

Data entry - Brian

Weigh plants - Sarah

GC run LTER20250112S17BGC had a vial set shift on one sheet. This was recorded on the sheet and at data entry. All vials run in sequential order on the GC. This is just for reference in case you want to verify that results line up with sites (but again, this should have been fixed on tech data entry of sampling forms): vial set 5,6,7,8 shifted to slots 9,10,11,12; vial set 9,10,11,12 shifted to slots 17,18,19,20; and, vial set 17,18,19,20 shifted to slots 5,6,7,8.

Grind plants - Brian, Slater

Pulverize SWF deep core soil - Slater, Alyssa

Data entry - Alyssa, Brian

Pack CN - Olivia

Data entry - Slater, Brian, Sarah

Weigh and grind plants - Alyssa, Olivia, Brian

Weigh plants - Olivia, Alyssa

Finish BAF subsampling, blow out Torit filters, grind plants - Slater

Pack CN - Brian

BAF BCSE plants subsampling - Olivia

Weigh plants - Slater

Data entry, WPS training - Alyssa

Weigh and grind plants - Sarah, Slater

Pack CN - Olivia

Acid washed dishes.

Weigh and grind plants - Brian, Olivia

Finish weigh roots, BAF subsampling - Jon

Pack CN - Slater, Jon

Weigh and grind plants - Sarah, Jon

Pulverize SWF soil -

Weigh roots, pack CN - Slater, Olivia

Weigh plants - Brian

Grind plants, pulverize SWF 2024 deep core soil - Slater, Olivia

Weigh 2024 Switchgrass Nitrogen Rate Experiment deep core roots - Jon

Weigh plants - Brian, Slater

Grind plants - Jon, Olivia

BAF subsampling - Olivia

Weigh 2023 Switchgrass Biodiversity Experiment deep core roots - Brian, Jon

Pack CN - Slater

Pack CN - Stacey, Olivia, Brian

Weigh plants - Brian, Olivia

Grind plants - Slater

BAF subsampling (BCSE harvest 2021) - Sarah

Weigh 2023 Switchgrass Biodiversity Experiment deep core roots - Jon

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Acid washed dishes.

Pack CN - Stacey, Slater

Weigh plants - Jon

Grind plants - Olivia

LTAR gas vial, tote prep - Slater

BAF subsampling (BCSE harvest 2020) - Brian

Pack CN - Brian, Olivia

Weigh plants - Olivia, Brian

Grind plants - Sarah, Slater

BAF subsampling (BCSE harvest 2019) - Slater, Jon

BCSE gas vial, tote prep - Jon

Dishes -

Pack CN - Slater, Brian

Weigh plants - Brian, Olivia

Grind plants - Slater

Lab clean-up, dump soil buckets, weigh dry tins - Sarah

LTAR gas data entry - Jon, Olivia

Archive LTAR air-dried soil -

Filter LTAR leachate; collection included many more samples than 11/24-25, so those archive samples were discarded and this collection was archived in it’s place. Also, subsamples from ASP3 and BAU2 leachate were delivered to Younsuk Dong and Subhasis Giri on campus by Dave Weed for phosphorus analysis at Brook’s request. - Slater, Sarah

Filter LTAR soil extracts - Slater, Sarah

Finish sieving LTAR soil - Jon, Alyssa

Weigh LTAR soil for extraction and moisture - Jon, Alyssa, Brian, Sarah

LTER gas vial/tote prep, weigh dry soil tins, dishes - Slater, Brian, Jon, Sarah

Start sieving LTAR soil - Alyssa, Slater, Brian, Jon, Sarah

Dishes, LTAR gas data - Slater, Brian, Olivia, Jon

Filter BCSE subplot soil extracts - Alyssa, Brian, Olivia

Weigh BCSE subplot soil for extraction and moisture, plus subsample G1-3 main and subplot to send to A&L - Slater, Jon

LTAR gas data - Brian, Jon

Dishes - Brian, Slater

Sieve BCSE subplot soil -

Filter BCSE main soil extracts, dishes -

Finish sieving BCSE main soil - Jon, Brian

Weigh BCSE main soil for extraction and moisture - Jon, Brian, Slater

Filter MLE MC soil extracts - Jon, Slater, Brian

Prepared 1M KCL, standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

GC sequence LTAR20251126S29BGC had two issues with vial order submitted in trays. Vials 100 and 101 were switched in trays, and vials 135 and 136 were switched in trays. All GC vials were run sequentially on instrument.

Weigh MLE MC soil (sampled 11/25/2025) for extraction and moisture - Brian, Jon

Sieve BCSE main soil - Alyssa, Sarah, Olivia, Slater, Jon, Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared 1M KCL.