Projectlog Entries List

Sticky trap (re)training on Teams - Emily, Olivia, Sam, Jon, Brian

Dishes - Brian

Filter LTER leachate, filter LTER soil extracts - Sam, Jon, Emily, Glori

Prep LTAR gas vials - Olivia, Brian

Prep LTER gas vials, totes - Stacey

Weigh LTER soil for extraction and moisture - Sam, Jon

WPS Zoom - Sam

Dishes - Jon

Grind SBE 2025 plants -

Prepared 1M KCL.

Sieve LTER soil - Brian, Jon, Emily, Sam

Dishes, vial prep - Olivia

Safety training - Bella

Finish sorting ASP2 plants - Brian, Olivia, Sam

Dishes - Jon

Prep gas vials for fertilization side project with Kevin, looking at gas fluxes over knifed-in vs. surface applied nitrogen - Olivia

Measure and filter LTAR leachate - Brian, Jon, Emily

Prep BCSE gas vials, totes - Sam, Emily

Prep LTAR gas vials - Stacey

Onboarding - Sam

Dishes - Brian

Sort ASP2 plants - Alyssa, Jon

Dishes - Jon

Sort ASP2 plants - Emily, Stacey

Sort ASP2 plant samples - Emily, Brian, Jon

Sequence LTAR20260423S6BGC had two samples switched in trays submitted from field techs. Samples were all run in sequential order on the GC, but in trays, samples 83 and 84 were swapped from their correct positions.

Filter LTER leachate - Jon

Filter BCSE, LTAR soil extracts, dishes - Stacey, Brian, Emily, Sarah

Prepared 1M KCL.

Weigh BCSE 4/24/2026 and LTAR April soil for extraction and moisture - Brian, Olivia

GC sequence BCSE202604100424S2S3BGC had samples submitted out of order in the field trays. All samples run in sequential order on GC. Field tray samples out of order as such: Series 2, samples 80 and 79 swapped; Series 3, in this order: ….21, 22, 30, 24, 25, 26, 27, 28, 29, 34, 31, 32, 33, 38, 35, 36, 37, 23, 39, 40….

Sieve LTAR soil - Alyssa, Sarah, Jon, Emily, Brian

GC Sequence LTAR20260416S5BGC issues: there was a subset of samples around vial 130 that yielded very large peaks on the FID detector, eluting after the methane peak, and sometimes bleeding into the subsequent injection. These samples were re-run under the same sequence name with “_B” suffix, and did not have the significant extra peaks as the first round. Kevin thought perhaps these vials had previously been used with acetylene block samples or some other contaminant. House air goes through a zero carbon filtration system, so should not have been an issue with these. In any case, the _B sample data may be better to use, as these did not have the interfering peaks that the first injection round had.

Sieve BCSE soil - Emily, Jon, Brian, Olivia

Dishes - Olivia, Brian

Pack CN - Jon

Grind SBE plants - Emily

Measure and filter LTAR leachate, dishes - Jon, Emily

Filter LTER soil extracts - Brian, Sarah

Weigh 2nd April LTER soil for extraction and moisture - Emily, Jon

LTAR, BCSE gas vial and tote prep - Brian

GC Sequence LTAR20260416S5BGC vial issues:
Sample vial 26 had a very crooked cap - was fixed to prevent GC needle breakage, but may have let lab air into sample in the process. Sample vials 139 and 140 were swapped in order in the field trays. Duplicate 3 sample vial was left upside down in the field tray (does this indicate no sample?).

Sieve LTER soil - Sarah, Alyssa, Emily, Jon, Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Dishes - Emily, Sarah

Grind SBE plants - Jon

Weigh urea for N-deposition fertilization - Brian

Measure and filter LTER leachate - Emily, Jon

WPS training - Olivia

Prep LTER and LTAR gas vials and totes - Emily, Jon

Dishes - Jon, Emily

GC Sequence LTER20260401S2BGC had some vials submitted out of order in the field trays. All samples were run in sequential order on the GC. Vials submitted out of order were as follows: …148, 150, 149, 151, 152… and …241, 242 ,247, 244, 245, 250, 251, 248, 249, 246, 243, 252, 253…

Dishes - Brian

BCSE and LTAR gas vial and tote prep - Brian, Sarah

LTER soil and LTAR Lysimeter filtering - Brian, Emily, Jon

Prepared 1M KCL, standards.

Lachat run LTER Lysimeter 20250506. -Sample DF3-1 was missing from set. Vial DF3-3 should not exist, ran as sample DF3-1.

LTER Soil extraction - Emily, Olivia

Dishes- Jon

Weigh and filter LTER Lysimeters - Jon, Emily

Acid washed dishes.

Lachat run LTER Lysimeter 20250421. Run notes:

• Samples T1 R2-2 and T7 R2-1 should have samples, but no vial was in sampled box, skipped.
• Sample CF1-1 has no vial, should exist. Vial labeled as CF1-6 does not have enough volume listed to filter, ran as CF1-1.

GC Sequence LTAR202603250402S2S3BGC had one set of sample vials out of order, as submitted by Techs. Vials were in no discernable order for that last rack so recorded in this order: 189,190,191,172,169,186,187,176,173,182,183,180,177,178,179,184,181,174,175,188,185,170, 171,192. Could be read in the opposite order as well? Anyhow, all samples run on GC in sequential order. Will look to see if this rack simply got jumbled up in the transport from field to lab.

Filter LTAR soil extracts - Jon

Prepared 1M KCL, Lachat run LTER Lysimeter 20250408.

Replaced end filter and 4th cartridge filter on Barnstead nanopure. Ran water test on Lachat, filter seems to be functioning normally again.

Weigh LTAR soil for extraction and moisture - Brian, Olivia

Prep LTER, LTAR gas vials - Emily

Sieve LTAR soil - Alyssa, Brian, Jon, Emily

Measure and filter LTAR leachate; dishes - Brian, Olivia

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Finished GLBRC Microplot soil 20251203 Lachat run and Re-extracted GLBRC MLE MC (Lake City) soil 20251125.

Filter LTER soil extracts - Brian, Sarah

Prepared 1m KCL. Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Weigh LTER soil for extraction and moisture - Olivia, Brian

LTAR gas vial prep - Jon

Dishes - Brian, Jon

Sieve March LTER soil - Alyssa, Sarah, Brian, Jon, Stacey

Measure and filter LTER leachate - Jon, Brian

Grind SBE plants - Olivia

Grind SBE plants - Brian

Pack LTAR 2023 plants - Jon

Dishes, lab cleaning - Sarah

Grind SBE plants - Jon

Pack LTAR 2023 plants - Brian

GC Sequence LTAR20260312S1BGC had a few samples submitted out of order. All samples run in numerical sequence on GC. Samples Dup3 and Dup4 were swapped with vials 3 and 4 as submitted. They were swapped back in proper order on GC run. Another sample out of order towards the end as such: ….112, 116, 113, 114, 115, 117, 118….

Safety training - Brian, Jon, Sarah

Fall project wrap-up - Sarah

Lab cleaning - Alyssa, Stacey

Pack LTAR 2023 CN - Olivia

Dishes - Jon, Olivia

Grind SBE 2025 - Brian

Relabel 2019, 2021 BCSE G2 harvest archive jars - Jon

Inventory LTER leachate archive - Brian

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Dishes, grind SBE palnts, inventory LTER leachate samples - Sarah, Jon, Brian

Grind SBE plants - Olivia, Brian

Measure and filter LTAR leachate, save archive sample, dishes - Olivia, Jon

Grind SWF plants - Sarah, Jon

Grind LTAR 2025 plants - Sarah, Jon

One last check on LTER T7 microplot plant archive samples where ANPP records don’t match Archive records - Jon

Pack LTAR 2023 plant CN - Olivia

Grind LTAR 2025 plants - Sarah

Pack LTAR 2023 plant CN - Jon

Prep LTAR gas vials - Olivia, Alyssa

Grind LTAR plants - Sarah

GC sequence BCSE20260303S1BGC notes: All samples run in sequential order. One set submitted out of order in trays as follows: vial # ….44, 45, 47, 46, 48, 49….

Acid washed dishes, Replaced Cadmium column.

Prep BCSE gas vials and totes - Jon

Prepared standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Grind LTAR 2025 plants - Brian, Jon

Pack LTAR 2023 plant CN - Jon

Grind LTAR 2025 plants - Olivia

Pack LTAR 2023 plant CN - Brian

Relabel BCSE 2021 archive jars - Jon

Filter 8/20 soil extracts, dishes - Jon, Brian

Prepared 1m KCL

Lachat run LTER soil 20250806 (Re-extracted on 2/23/26 to compare to contaminated samples)

Grind LTAR 2025 plants - Jon

Reweigh LTER 8/20/2025 soil samples for extraction and moisture, initial results were good but reextracted to compare to other ammonium contaminated samples. Also, some of this soil warmed up when a walk-in stopped working, so interesting to compare initial to warmed samples. - Brian, Sarah

Relabel BCSE 2021 archive jars - Brian

Weigh dry soil tins, wash dishes - Sarah

Grind LTAR 2025 plants - Brian

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Jon

Filter 8/6 soil extracts - Jon, Brian

Prepared 1M KCL

Lachat run LTER T3 soil 20250723 (Re-extracted on 2/18/2026 due to KCl contamination)

Grind LTAR 2025 plants - Jon, Sarah

Reweigh LTER 8/6/2025 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts, results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Brian, Sarah

Relabel BCSE 2019, 2021 archive jars - Jon

Weigh dry soil tins, wash dishes - Brian

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Brian

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Brian

Filter 7/23/2025 soil extracts - Jon, Brian

Lachat run LTER T4 soil 20250929.

Prepared 1M KCL.

Grind LTAR 2025 plants - Sarah

Reweigh LTER 7/23/2025 T3, T4 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts; results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Jon, Brian

Finish grinding BCSE 2025 plants, start grinding LTAR 2025 plants - Olivia, Brian

Pack LTAR 2023 plant CN - Jon

Cleaning at field lab and trucks, start relabeling BCSE 2019 plant archive jars with labels Nick Thrower sent with vertical barcodes so scanner can read them (they were horizontal and went around the corner of the jars, so couldn’t be read by a scanner) - Brian

Grind BCSE plants - Jon

Pack LTAR plant 2023 CN - Brian

Finish subsampling SWF plants for BAF, cleaning - Sarah

Acid washed dishes. Lachat run LTER Soil 20250915.

Grind BCSE plants - Brian

Finish packing LTER plant 2023 CN, start packing LTAR 2023 plants - Jon, Olivia

GC Sequence BCSE20260205S25BGC vials submitted out of sequence by techs. All vials run in numerical sequence on GC. Vials submitted out of sequence as follows: …31,32,34,33,35,36… …50,51,56,53,54,55,52,57,58….

Grind BCSE plants - Jon

Pack LTER plant 2023 CN - Brian

GC Sequence LTER20260206S18BGC notes. Vials 13-16 were not sampled (techs couldn’t find chamber in the snow), but run on GC as empty vials to keep run in order. Tech notes that vials 73-112 contained swapped sets (i.e. vial 93-96 were used for sheet slots 73-76). These were corrected on the field note sheets and entered at the field note data entry to properly line up the vials with the sites

Grind BCSE plants - Brian

Pack LTER plant 2023 CN - Jon

Grind ASP6 POACEAE (combined grasses) on campus, deliver BCSE grain harvest samples to Chris - Jon, Brian

Grind BCSE plants - Olivia

Grind BCSE plants - Sarah

Pack CN - Brian

Sort ASP6 samples to grind on campus 2/10/2026 - Jon

Finish grinding LTER plants -

Prepare LTAR gas vials and totes -

Grind BCSE plants on campus - Brian, Sarah

Prepare BCSE gas vials and totes - Alyssa

Finish entering T7 microplot plant archive data - Jon

Grind LTER plants - Olivia, Brian

Prepare LTER gas vials and totes - Brian

T7 microplot plant archive data entry - Jon

Acid washed dishes, prepared Standards.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER T4 soil 20250723. Contamination in KCL blanks in NH4 results, not uploaded.

Grind BCSE plants on campus - Jon, Brian

Grind LTER plants - Sarah

Grind BCSE plants on campus - Alyssa, Jon

Grind LTER plants - Brian, Olivia

BAF subsampling - Slater

Pack CN - Slater, Brian

Weigh SBE plants, grind LTER plants - Slater, Brian

T7 microplot plant archive data entry - Jon

Pack CN - Olivia

Weigh SBE plants, grind LTER plants - Sarah, Brian, Slater

T7 microplot plant archive - Jon

Pack CN - Slater, Brian

Reconditioned Cadmium Column, Acid washed dishes.

Lachat run LTER T4 soil 20250527

Weigh SBE plants - Slater, Jon

Grind LTER plants - Jon, Slater

Pack CN - Olivia

Weigh SBE plants - Brian

Grind LTER plants - Slater, Sarah

T7 microplot plant archive - Jon

Data entry, BAF subsampling - Sarah, Slater

Grind LTER and weigh SBE plants - Brian

Weigh plants - Brian

Grind plants - Olivia, Slater

Continue checking T7 microplot plant archive - Jon

Pack CN - Slater

Grind and weigh plants - Slater, Sarah, Brian

Check LTER T7 microplot plant archive jars going back to the start of the project against a spreadsheet created from ANPP data to establish a T7 microplot plant archive data set similar to what we already have for the LTER main plots. - Jon

Pack CN - Slater, Sarah