Projectlog Entries List

Prepared 1M KCl

Lachat run LTER Lysimeter 20260402. Notes from run:

• Three vials labeled T3 R3 1, 2, and 3 were ran as T5 R3 1, 2, and 3. Flasks for T3 R3 and T5 R3 were swapped when evacuating samples. Weights were adjusted when entering in data file to the correct location, but vials were sampled incorrectly.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

LTER Grad Fellow Symposium

Pack CN - Glori

Data entry - Emily, Jon

LTER field lab to do list (cleaning, organization, dump old samples) - Jordyn, Sam, Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER Lysimeter 20260319. Notes from run:

• Vial labeled T2 R4 1 should not exist, not enough volume listed. Ran as Vial T3 R4 1, that was not present and should exist.
• Vial T CF R2 1 missing from box, not run (no data notes)
• Vial T6 R3 3 present, should not exist. Not enough volume in vial to run.

Replaced Cadmium Column, acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopore H2O.

Weigh LTAR plants - Jon, Jordyn

Sort ASP1 forage - Emily, Brian, Bella, Jon, Sam, Olivia

Pack CN - Sam, Bella

Dishes, lab clean-up - Jordyn, Glori

NAS vial prep and data entry - Olivia

Measure and filter LTAR leachate, filter LTER soil extracts, LTAR and BCSE gas vial prep - Jordyn, Glori, Emily, Brian

Sort ASP1 forage - Sam, Jon

Prepared Standards.

Lachat run LTAR Lysimeter 20260616. Notes from run:

• Vial labeled ASP2 R1 N should not exist, not enough volume. Ran as vial ASP2 R2 N, that should exist and is missing.
• Vial ASP6 R2 S was missing from box and should exist, not run.

Prepared 1M KCl

Sieve LTER soil -

Weigh LTER soil for extraction and moisture - Alyssa,

Sort ASP1 forage, empty ovens - Alyssa

Make new LTER gas vials; all previous vials are awaiting analysis due to troubles with the old GC and switch over to a new GC - Alyssa, Olivia

Measure and filter LTER leachate - Sam, Jordyn, Glori

Reshake then filter LTER T3, T4 soil extracts - Emily, Brian

Prep LTAR gas vials, totes - Jordyn, Glori

Weigh T3, T4 soil for extraction, moisture and T21 whirlpaks (90-100g/whirlpak) - Olivia, Brian, Jordyn

BCSE gas tote prep, dishes - Jordyn, Brian

Dishes - Jordyn

BCSE new vial prep - Glori

Field lab clean-up - Brian

Dishes - Jordyn

Measure and filter LTAR leachate - Sam, Glori

Filter LTER soil extracts - Jordyn, Jon

Weigh LTER soil for extraction, moisture and T21 incubation - Glori, Jordyn, Brian around 11

BCSE and LTAR gas vial prep - Jordyn

Sieve LTER soil - Sam, Emily

Finish prepping LTER gas vials and totes - Brian, Bella, Jordyn, Olivia, Sam

Prep more NAS gas vials - Olivia

GHG talk from Kevin - Alyssa, Bella, Brian, Emily, Glori, Jon, Jordyn, Sam

Weigh and filter LTER leachate - Olivia, Jordyn

Filter LTAR and BCSE soil extracts - Glori, Brian

Prepared 1M KCL.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTAR Lysimeter 20260519. Notes from run:

• Samples ASP1 R4 C, S, and ASP3 R3 C were not present, not analyzed.

Weigh LTAR and BCSE soil for extraction and moisture - Glori, Jordyn

BCSE gas vial, tote prep - Glori

Grind LTAR plants - Brian

Pack CN - Jordyn

Grind plants - Glori, Sam

Sieve LTAR and BCSE soil -

Pack CN - Olivia

Archive LTER air-dried soil -

Dishes - Sam

Pack CN - Brian, Emily

Grind plants - Emily, Olivia

Grind LTER 2026 plants - Jordyn, Jon

Pack CN - Glori

Dishes - Sam

Data - Emily

Measure and filter LTAR leachate - Brian, Jordyn

Filter LTER soil extracts - Jon, Glori

Pack CN - Jon

Grind LTER 2026 plants - Sam

BCSE gas vial, tote prep - Glori

Weigh 2nd June soil for extraction and moisture - Jordyn, Glori

Grind LTER plants - Alyssa train Jordyn and Glori

Pack CN - Jordyn

Prepared 1M KCL. Replaced all Lachat reagent tubing.

Data entry - Brian

CN packing - Sam

Dishes - Glori

Weigh plants - Jordyn

LTER gas vial, tote prep - Glori

Weigh plants - Jordyn

LTER field lab clean-up, organization -

Measure and filter LTER leachate -

BCSE gas vial and tote prep -

Filter NAS soil extracts for Olivia -

Field lab cleaning and organization - Brian, Jon, Emily, Sam, Glori, Bella, Jordyn

Pack CN - Jordyn, Bella

Weigh LTER T3, T4 cover crop plants, then LTAR ASP2 - Bella, Brian, Jordyn

Data entry - Brian, Jordyn

Dishes - Glori

Weigh soil tins - Jordyn

Pack CN - Emily, Olivia

Pack CN - Jon, Sam

LTAR gas vial, tote prep - Glori

Dishes - Jon, Jordyn

Sort ASP1 plants - Brian, Emily

Sort ASP1 plants - Olivia am/pm

Measure and filter LTAR leachate - Jordyn, Glori

Filter LTER Soil extracts - Emily, Bella

Prep BCSE gas vials, totes - Emily, Bella

Prepared 1M KCL. Acid washed dishes.

Weigh LTER soil for extraction and moisture - Glori, Sam

Dishes - Jordyn, Glori

Measure and filter LTER leachate - Alyssa, Jon, Sam

LTER gas vial prep - Jon

Prepared 1M KCL.

Weigh LTAR soil for extraction and moisture - Brian, Olivia

Field lab clean-up, dump old samples, put soil (LTER 4/6/2026 and T3, T4 5/18/2026) out to air dry, organize sticky trap binder, weigh dry soil tins - Jon, Olivia, Emily, Jordyn, Glori, Brian

Dishes - Jon, Emily

Finished grinding SBE plants - Sam

BCSE gas vial, tote prep; WPS training - Jordyn

LTAR gas vial, tote prep - Glori, Olivia

Measure and filter LTAR leachate - Jordyn, Bella, Alyssa trained

Filter LTER T3, T4 soil extracts - Glori, Brian

Grind SBE - Sam

Data entry - Olivia

Weigh T3, T4 soil for extraction and moisture - Alyssa, Bella, Jordyn, Glori

WPS site-specific training with Brook on Zoom - Glori, Bella, Jordyn

Safety training - Jordyn

Dishes, data entry - Glori

Prepared 1M KCL

Onboarding, training - Jordyn (first day)

Grind SBE plants - Jon

Sieve LTER T3, T4 soil - Emily, Jon, Sam, Brian, Bella, Glori

Field lab clean up - Bella, Glori, Brian

Dishes, weigh dry tins - Olivia

WPS Zoom - Bella, Glori

Sticky trap (re)training on Teams - Emily, Olivia, Sam, Jon, Brian

Dishes - Brian

Filter LTER leachate, filter LTER soil extracts - Sam, Jon, Emily, Glori

Prep LTAR gas vials - Olivia, Brian

Prep LTER gas vials, totes - Stacey

Weigh LTER soil for extraction and moisture - Sam, Jon

WPS Zoom - Sam

Dishes - Jon

Grind SBE 2025 plants -

Prepared 1M KCL.

Sieve LTER soil - Brian, Jon, Emily, Sam

Dishes, vial prep - Olivia

Safety training - Bella

Finish sorting ASP2 plants - Brian, Olivia, Sam

Dishes - Jon

Prep gas vials for fertilization side project with Kevin, looking at gas fluxes over knifed-in vs. surface applied nitrogen - Olivia

Measure and filter LTAR leachate - Brian, Jon, Emily

Prep BCSE gas vials, totes - Sam, Emily

Prep LTAR gas vials - Stacey

Onboarding - Sam

Dishes - Brian

Sort ASP2 plants - Alyssa, Jon

Dishes - Jon

Sort ASP2 plants - Emily, Stacey

Sort ASP2 plant samples - Emily, Brian, Jon

Sequence LTAR20260423S6BGC had two samples switched in trays submitted from field techs. Samples were all run in sequential order on the GC, but in trays, samples 83 and 84 were swapped from their correct positions.

Filter LTER leachate - Jon

Filter BCSE, LTAR soil extracts, dishes - Stacey, Brian, Emily, Sarah

Weigh BCSE 4/24/2026 and LTAR April soil for extraction and moisture - Brian, Olivia

Prepared 1M KCL.

GC sequence BCSE202604100424S2S3BGC had samples submitted out of order in the field trays. All samples run in sequential order on GC. Field tray samples out of order as such: Series 2, samples 80 and 79 swapped; Series 3, in this order: ....21, 22, 30, 24, 25, 26, 27, 28, 29, 34, 31, 32, 33, 38, 35, 36, 37, 23, 39, 40....

Sieve LTAR soil - Alyssa, Sarah, Jon, Emily, Brian

GC Sequence LTAR20260416S5BGC issues: there was a subset of samples around vial 130 that yielded very large peaks on the FID detector, eluting after the methane peak, and sometimes bleeding into the subsequent injection. These samples were re-run under the same sequence name with "_B" suffix, and did not have the significant extra peaks as the first round. Kevin thought perhaps these vials had previously been used with acetylene block samples or some other contaminant. House air goes through a zero carbon filtration system, so should not have been an issue with these. In any case, the _B sample data may be better to use, as these did not have the interfering peaks that the first injection round had.

Sieve BCSE soil - Emily, Jon, Brian, Olivia

Dishes - Olivia, Brian

Pack CN - Jon

Grind SBE plants - Emily

Measure and filter LTAR leachate, dishes - Jon, Emily

Filter LTER soil extracts - Brian, Sarah

Weigh 2nd April LTER soil for extraction and moisture - Emily, Jon

LTAR, BCSE gas vial and tote prep - Brian

GC Sequence LTAR20260416S5BGC vial issues:
Sample vial 26 had a very crooked cap - was fixed to prevent GC needle breakage, but may have let lab air into sample in the process. Sample vials 139 and 140 were swapped in order in the field trays. Duplicate 3 sample vial was left upside down in the field tray (does this indicate no sample?).

Sieve LTER soil - Sarah, Alyssa, Emily, Jon, Brian

Dishes - Emily, Sarah

Grind SBE plants - Jon

Weigh urea for N-deposition fertilization - Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Measure and filter LTER leachate - Emily, Jon

WPS training - Olivia

Prep LTER and LTAR gas vials and totes - Emily, Jon

GC Sequence LTER20260401S2BGC had some vials submitted out of order in the field trays. All samples were run in sequential order on the GC. Vials submitted out of order were as follows: ...148, 150, 149, 151, 152... and ...241, 242 ,247, 244, 245, 250, 251, 248, 249, 246, 243, 252, 253...

Dishes - Jon, Emily

Dishes - Brian

BCSE and LTAR gas vial and tote prep - Brian, Sarah

LTER soil and LTAR Lysimeter filtering - Brian, Emily, Jon

LTER Soil extraction - Emily, Olivia

Dishes- Jon

Prepared 1M KCL, standards.

Lachat run LTER Lysimeter 20250506. -Sample DF3-1 was missing from set. Vial DF3-3 should not exist, ran as sample DF3-1.

Weigh and filter LTER Lysimeters - Jon, Emily

GC Sequence LTAR202603250402S2S3BGC had one set of sample vials out of order, as submitted by Techs. Vials were in no discernable order for that last rack so recorded in this order: 189,190,191,172,169,186,187,176,173,182,183,180,177,178,179,184,181,174,175,188,185,170, 171,192. Could be read in the opposite order as well? Anyhow, all samples run on GC in sequential order. Will look to see if this rack simply got jumbled up in the transport from field to lab.

Acid washed dishes.

Lachat run LTER Lysimeter 20250421. Run notes:

• Samples T1 R2-2 and T7 R2-1 should have samples, but no vial was in sampled box, skipped.
• Sample CF1-1 has no vial, should exist. Vial labeled as CF1-6 does not have enough volume listed to filter, ran as CF1-1.

Filter LTAR soil extracts - Jon

Weigh LTAR soil for extraction and moisture - Brian, Olivia

Prep LTER, LTAR gas vials - Emily

Prepared 1M KCL, Lachat run LTER Lysimeter 20250408.

Replaced end filter and 4th cartridge filter on Barnstead nanopure. Ran water test on Lachat, filter seems to be functioning normally again.

Sieve LTAR soil - Alyssa, Brian, Jon, Emily

Measure and filter LTAR leachate; dishes - Brian, Olivia

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Finished GLBRC Microplot soil 20251203 Lachat run and Re-extracted GLBRC MLE MC (Lake City) soil 20251125.

Filter LTER soil extracts - Brian, Sarah

Prepared 1m KCL. Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Weigh LTER soil for extraction and moisture - Olivia, Brian

LTAR gas vial prep - Jon

Dishes - Brian, Jon

Sieve March LTER soil - Alyssa, Sarah, Brian, Jon, Stacey

Measure and filter LTER leachate - Jon, Brian

Grind SBE plants - Olivia

Grind SBE plants - Brian

Pack LTAR 2023 plants - Jon

Dishes, lab cleaning - Sarah

Grind SBE plants - Jon

Pack LTAR 2023 plants - Brian

GC Sequence LTAR20260312S1BGC had a few samples submitted out of order. All samples run in numerical sequence on GC. Samples Dup3 and Dup4 were swapped with vials 3 and 4 as submitted. They were swapped back in proper order on GC run. Another sample out of order towards the end as such: ....112, 116, 113, 114, 115, 117, 118....