Sequence LTER20250620S8BGC issues: All GC vials run sequentially, some samples submitted out of order in trays. Vials 41 and 57 were swapped (i.e. …39, 40, 57, 42, 43… and …55, 56, 41, 58, 59…) One other set of vials submitted out of order as follows: …105, 106, 111, 108, 109, 110, 107, 112, 113…

BCSE20250530060506130619S7S8S9S10BGC sequence had one exception: Series 10 had a note that vials 8 and 5 were swapped in the field. Therefore, for series 10, they were run in that order on the GC, i.e. …3, 4, 8, 6, 7, 5, 9, 10….

BCSE gas vial, tote prep - Sarah

Grind plants, pulverize soil - Sam,

Archive air-dried soil -

Pack CN - Brian

Data entry -

Grind plants, pulverize soil - Olivia, Jon,

LTAR gas vial, tote prep, pack CN - Olivia

Filter BCSE soil extracts -

Archive air-dried soil - Slater

Dishes -

BAF subsampling - Trajana

Sort ASP6 R5 plants to species - Slater, Brian, Trajana

Weigh dry tins, BCSE soil for extraction and moisture - Trajana, Olivia

Grind plants, pulverize soil - Sara, Olivia

Dishes - Jon

Pack CN - Brian

Grind plants and pulverize soil -

Dishes -

LTER gas vial, tote prep - Brian, Sarah

Grind plants, pulverize soil - Sam,

Dishes -

Measure and filter LTER leachate -

Filter LTER extracts -

Dishes -

Acid washed dishes.

Weigh LTER soil for extraction and moisture - Sam, Jon

Grind plants, pulverize soil -

LTAR, BCSE gas vial prep -

Dishes

Sieve LTER soil -

Grind plants, pulverize soil - Trajana, Slater, Sam

Dishes - Jon, Olivia, Trajana

Acid washed dishes.

Grind plants -

BCSE gas vial, tote prep -

Pulverize soil -

Pack CN, dishes, data, BAF subsampling -

Measure and filter leachate - Sarah, Jon

Dishes - Sam

LTAR gas vial, tote prep - Olivia, Trajana

Filter LTAR soil extracts - Sarah, Jon

Grind plants, pulverize soil - Trajana, Olivia

GC Sequence LTER20250606S7BGC had just a few samples submitted out of order: All vials run in numerical sequence on GC. Submitted vials out of order in trays: …232, 234, 233, 235, 236….

Weigh LTAR soil for extraction and moisture - Sarah, Slater

Grind plants, pulverize soil, archive air-dried soil, dishes, data, BAF subsampling -

Sieve LTAR soil -

Grind T7 REX 2023 plants and organize samples - Brian, Olivia

Pulverize BCSE 2022 deep core soil - Sam

BAF subsampling BCSE 2022 deep core soil - Jon

Dishes - Sarah

Finish sorting ASP2 plants -

Dishes, data -

LTER gas vial and tote prep -

Sort ASP2 plants -

GC sequence LTAR202505220529S10S11BGC sample issues: All samples run on GC in sequential order. Series 10 vials submitted out of order: …80, 81, 86, 83, 84, 85, 82, 87, 88…. Series 10 vial 103 broke in field, so sample run is “dummy” sample of air. Series 11 vials submitted out of order: …6, 7, 11, 8, 9, 10, 7, 12, 13, 14, 19, 16, 17, 18, 15, 20, 21…. and …123, 124, 126, 125, 127, 128…

Measure and filter LTER leachate - Sarah, Slater

Filter LTER soil extracts -

LTAR, BCSE gas tote pre -

Sort ASP2 plants -

Sieve LTER soil - Alyssa, Clara, Sarah, Trajana

Weigh LTER soil for extraction and moisture - Alyssa, Clara, Sarah, Trajana

Sort ASP2 plants -

LTAR, BCSE gas vial prep - Stacey

Grind plants - Olivia

Measure and filter leachate, dishes - Sarah, Jon

Sort ASP2 plants - Brian, Olivia, Sam, Andie

Grind plants - Slater

Sequence LTER20250523S6BGC notes: SF1-1 and SF1-3 were switched around at sampling time - see vial # notes on setup file sheets Vials submitted out of order, but run in sequential order on the GC (may or may not have anything to do with the first note). Out of order vials noted as follows: …127, 128, 129, 132, 130, 131, 133, 134… …147, 148, 153, 154, 155, 156, 149, 150, 151, 152, 156, 157… (guessing these correspond to SF1-1 and SF1-3 mixup) …241, 242, 247, 248, 245, 246, 251, 252, 249, 250, 243, 244, 253, 254, 255…

Measure and filter LTAR leachate, dishes - Jon, Sarah

Grind plants -

Sort ASP2 plants -

Filter LTER soil extracts - Alyssa, Sarah

Prepared standards, prepared 1M KCL.

Acid washed dishes.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Weigh T3, T4 soil for extraction and moisture - Sarah, Andie, Olivia

LTAR gas vial, tote prep - Olivia

Grind plants -

Subsample 2022 BCSE deep core soil to send to BAF - Andie

Dishes, data -

Sieve T3, T4 soil - Sarah, Andie

WPS - Sam

Sequence BCSE2025043005140522S4S5S6 had a couple tech notes for series 6: Vial 49 was lost during sampling, so a replacement vial was used to collect the sample - this was run in sequential order on the GC. Ambient air samples were not collected - empty vials were run on the GC anyway for continuity of the run.

Filter BCSE soil extracts, dishes -

Grind T7 REX 2023 plants -

Sieve and weigh BCSE soil for extraction and moisture -

LTER gas vial and tote prep -

Measure and filter LTER leachate -

Filter LTER soil extracts - Trajana, Andie

Grind plants - Olivia

Prepared standards, prepared 1M KCL.

Lachat run GLBRC Scale up Lux Arbor 20241202, 20241216 - Blanks from 20241202 showed contamination in NH4 (L1 and L3 samples), not uploaded.

Reconditioned Cadmium Column.

Weigh LTER soil for extraction and moisture - Sam, Slater

Gas vial and tote prep, dishes - Sarah

BAF subsampling (2024 BCSE peak plants from archive jars) - Trajana

Grind plants - Andie

GC Run Sequence LTAR202505080515S8S9BGC notes: Dave dropped and broke vial 38 for Series 8. Replaced with dummy sample (lab air, not pressurized).

Site-specific training for techs - Stacey, Alyssa, Slater, Brian, Olivia, Trajana, Sam, Andie, Sarah , Jon

Sieve LTER soil -

Grind plants -

BAF subsampling (2023 BCSE plants) - Brian

Sticky trap training with Christie - Sam, Slater, Brian, Jon

Power was lost overnight 5/15-5/16. Backup generator worked intermittently throughout the day but stopped working for several hours beginning around 2:30pm. Received message that emergency backup generator power was restored at about 10pm 5/16/2025. Then Saturday 5/17/2025 8am this message from IPF “Regular power has been restored.  However, IPF will be keeping the academic campus buildings on back up power.  We are planning to take advantage of already contracted back up generators so that we can further assess the failure of our normal back up power system.”.

BAF subsampling (2023 BCSE plants) - Trajana

Grind plants -

Dishes -

WPS Zoom and site-specific (w/Brook) training - Sarah, Jon

Measure and filter LTAR leachate -

Filter LTAR soil extracts -

Grind plants and pulverize soil -

GHG presentation/training from Kevin - Alyssa, Slater, Brian, Trajana, Sam, Olivia, Sarah, Jon, Clara

Weigh LTAR soil for extraction and moisture; BCSE and LTAR gas vial and tote prep - Sam, Trajana

Sieve LTAR soil -

WPS Zoom training - Olivia, Slater

Sequence BLESH-UDF202505S4S5S6BGC cap issues. Series 4, vial 389 had a loose cap - may have lost all sample, tightened in lab and run. Series 6, vial 95 had a cap screwed on extremely crooked - would likely cause sampling issues. Corrected in lab, but may have allowed gas sample to escape in the process. Put tape note on side of cap to discard before next use.

Dishes -

Put LTER 4/7/2025 soil out to air dry -

Filter LTER leachate and LTER soil extract, dishes - Slater, Sam, Trajana, Olivia

Prepared 1M KCL.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Weigh LTER soil for extraction and moisture - Alyssa, Brian

Prep LTAR and LTER gas vials, totes -

Grind plants, pulverize soil -

GC Sequence run LTER20250425S4BGC: Note on sample trays from techs: “vial swap for some FROG vials” All samples were run in sequential order, but some vials swapped and submitted to lab as follows: ….39, 40, 57, 42, 43, 44, 61, 46, 47, 48, 65, 50, 51, 52, 69, 54, 55, 56, 41, 58, 59, 60, 45, 62, 63, 64, 49, 66, 67, 68, 53, 70, 71, 72….. and ….244, 245, 248, 247, 246, 249, 250….

Sieve LTER soil -

Sequence BLESH202504S1S2S3BGC had some hiccups - mainly in skipped samples. Samples were likely skipped due to having the Excel Results file open to look at data, as the GC was running. Samples 218, 219, and 354 were skipped for the methane portion of the run. Sample 199 was skipped for the CO2/N2O portion. At the same time, it appeared that one line of data was appended without assigning it a vial number - so everything was off one row (verified by check standards). This happened around the missing 218 vial mark. The Results file sequence lines were corrected and saved as a file with the sequences name. Skipped samples listed above were re-analyzed in groups of 4 with corresponding B and C versions of the Sequence/File name.

Finish sorting ASP3 and ASP4 plant samples - Stacey, Brian, Andie, Slater, Sam

Filter BCSE soil extracts, dishes - Olivia, Trajana

Sequence LTAR202504240430S6S7BGC has two issues.

1. Discovered Vial 132 on Series 6 had a loose cap - so loose that it came off when running hand over the top of filled sample rack
2. Series 7 had an issue understood as the following: series started with the wrong vials. On 2nd time through sampling, this was discovered, so the series was initiated again with the correct vials. Therefore, vials should be in order, but each series starting with the following vials were started after 35-40 minutes with the lid on: vial 5, 9, 13, 17, 21. These times should be indicated on the field sheets. If not, email weedd@msu.edu for a copy.

Weigh BCSE soil, dishes - Sam, Trajana, Olivia, Slater

Sort ASP3 plants - Olivia, Slater

Sieve BCSE soil samples -

Filter LTAR leachate, dishes -

Sort ASP3 plant samples -

LTAR and BCSE gas vial and tote prep, dishes - Sam, Trajana

Noticed an issue with inconsistent check standards on LTAR202504100417S4S5BGC run. Some checks made before, some after, a nitrogen tank change-out. (N2 used in making up standards.) High standards, with no N2 addition, appear to be fairly consistent across the last 3 sample runs. A separate standard/check std run was completed after the LTAR run to test for this issue. This standard run would likely be better to use with the LTAR run. Standard run was STD_TEST20250424BGC.

Filter LTER soil extracts - Alyssa, Olivia

Prepared 1M KCL.

Weigh LTER soil for extraction and moisture - Brian, Trajana, Olivia, Alyssa

Dishes - Olivia

Sieve LTER soil - Alyssa, Slater, Brian, Trajana, Andie

LTAR202504100417S4S5BGC sample run on GC had vials submitted out of order on trays. All samples were run on GC in numerical order. Samples submitted out of order as follows: …4, 5, 9, 7, 8, 6, 10, 11, 12… and …135, 136, 140, 137, 138, 139, 141, 142….

BCSE202503310416S2S3BGC sample run on GC had a small set of samples submitted out of order on the trays. All vials were run on the GC in numerical order. Vials out of order on trays as follows: ….56, 57, 59, 58, 60, 61, 62, 63…..

Filter LTER leachate -

Grind SBE plants -

LTAR and LTER gas vial, tote prep; dishes - Slater

Finish subsampling archive for Jin Ho - Trajana

Acid washed dishes.

Archive air-dried LTAR R5 soil samples, subsample LTER soil archive for Jin Ho Lee (10g each from T3R1-6S5 2020, 2021, and 2022) -

Grind SBE plants, pulverize BCSE deep core soil, BAF subsampling -

Dishes, grind SBE plants, pulverize BCSE deep core soil, BAF subsampling - Brian, Olivia, Slater, Trajana

Measure, filter and archive LTAR leachate; one spring sampling and one fall sampling will be archived each year, 20mL sample with conical seal cap (same as LTER) -

Filter LTAR soil extracts -

Weigh LTAR soil for extraction and moisture - Slater, Trajana

Grind SBE plants and pulverize BCSE deep core soil - Olivia, Brian

Prep BCSE and LTAR gas vials and totes - Slater, Trajana

GC run LTAR202503260403S2S3BGC had several vials mixed up in one series 3 tray received from the techs. All vials run in sequential order on the GC. Here is the order presented in the trays for series 3: …23, 24, 25, 46, 47, 48, 29, 42, 43, 44, 33, 38, 39, 40, 37, 34, 35, 36, 41, 30, 31, 32, 45, 26, 27, 28, 49, 50, 51, 52, etc….

Sieve LTAR April soil - Slater, Brian, Trajana, Andie

Grind plants, pulverize BCSE deep core soil, weigh dry soil tins, dishes - Slater, Brian, Trajana, Andie

LTER Gas Series 3 - All + hydrosense (Alyssa, Andie) (Forest set DF2, CF2, SF3 split and sampled by two people due to issue with golf cart)

Dishes - Brian

Finish LTER Lysimeter filtering - Alyssa

Prep LTER gas totes, vials - Brian

BAF subsampling - Slater, Finish grinding GLBRC plants, mix air drying soil - Trajana

Filter LTER soil extracts - Brian, Slater

Measure and filter LTER lysimeter leachate - Andie, Trajana

Weigh LTER soil for extraction and moisture - Alyssa, Olivia, Slater

LTAR vial, tote prep - Olivia. BAF subsampling - Slater

Prepared 1M KCL Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER soil 20241023.

Dishes, Grinding Plants - Slater, Olivia

Grind plants and finish SWF deep core archiving and subsampling - Slater, Andie

LTAR gas vial and tote prep - Stacey

Grind plants, pulverize BCSE deep core soil, SWF deep core archiving and subsampling, field lab clean-up - Olivia, Slater, Andie

SWF deep core archiving and subsampling - Slater, Andie

Grind plants; pulverize BCSE DC soil; weigh, archive and subsample SWF DC soil - Slater, Trajana, Andie, Olivia

Dishes, BCSE gas vial prep, weigh dry soil tins - Brian

Filter LTER soil extracts, prep LTER gas vials/totes, dishes - Slater, Brian, Trajana

Sieve LTAR R5 soil, remove subsample to send to A&L for S1 package, set the rest out to air dry for archive - Stacey

Finish sieving LTER soil - Slater, Trajana

Weigh LTER soil for extraction and moisture - Brian, Olivia

Prep LTAR gas vials/totes, dishes - Brian, Olivia

Prepared 1M KCL, Reconditioned Cadmium Column.

Lachat run LTER T3 soil 10/21/2024.

Sieved LTER soil - Alyssa, Slater, Brian, Trajana, Andie

Weigh and grind plants, pulverize BCSE deep core soil - Slater, Trajana, Brian, Olivia

Finish filtering LTAR leachate, dishes - Brian

Weigh and grind plants, archive air-dried soil, pulverize BCSE deep core soil - Slater, Trajana, Brian, Olivia

Filter LTAR soil extracts - Brian, Trajana

GC run BCSE20250307S1BGC had one series of vials submitted out of numerical order. All vials run on the GC in numerical order, but submitted series were …60, 62, 61, 63, 64, 65, etc… (so just 61 and 62 submitted out of order, but subsequently run in numerical order).

Prepared standards, prepared 1M KCL.

Lachat run CLE inorganic N (Hammond) June 2024 soil.

Weigh LTAR soil for moisture and N-extraction -

Weigh and grind plants, pulverize BCSE deep core soil, subsample/archive SWF deep core soil - Slater, Trajana, Brian, Olivia

Weigh ASP6 biomass sampled on 3/12/2025 - Stacey

Sieve LTAR soil - Alyssa, Slater, Trajana, Brian

Weigh and grind plants, pulverize 2022 BCSE deep core soil - Slater, Trajana, Brian

Weigh and grind plants, pulverize 2022 BCSE deep core soil (Alyssa trained), subsample and archive SWF deep cores soil - Slater, Brian, Andie, Trajana, Olivia

Weigh and grind plants - Brian, Olivia

Subsample and archive SWF DC soil - Slater

Finish filtering LTER leachate, prep LTAR gas vials and totes - Alyssa

Weigh and grind plants - Andie, Brian

Subsample and archive SWF DC soil - Trajana, Slater

Measure and filter LTER leachate - Brian, Trajana, Olivia

Weigh SWF deep core moisture samples - Slater

Weigh and grind plants - Trajana, Slater

Weigh SWF deep core moisture samples - Andie, Brian

Finish processing SWF deep cores - Slater, Olivia

Weigh and grind plants - Trajana, Brian