Filter LTAR soil extracts, dishes - Slater, Emily

Pack CN - Stacey

Weigh LTAR soil for extraction and moisture - Brian, Ainsley

LTER gas vial and tote prep - Slater

Transect data entry - Jon

Pack CN - Stacey

Transect data entry - Brian, Ainsley

Weigh plants - Sarah

Pack CN - Stacey

Pack CN - Stacey

Weigh plants - Brian, Jon

Plant species composition transect data entry - Emily, Slater, Ainsley, Brian

Weigh dry soil tins, dishes - Jon

Plant species composition transect data entry 2025 - Olivia, Brian

Dishes - Sarah

Weigh plants - Ainsley, Alyssa

Transect data entry - Ainsley

Transect data entry - Alyssa

Measure and filter LTAR leachate - Brian, Emily

Pack CN (LTER 2020 plants) - Stacey

Transect data entry - Olivia, Ainsley, Brian, Jon

LTAR gas vial and tote prep - Brian, Sarah

Filter LTER T3, T4 soil extracts - Slater, Sarah, Emily

Pack CN (LTER 2020 plants) - Stacey

Weigh T3, T4 soil for extraction and moisture - Brian, Ainsley, Jon

Measure and filter LTER leachate, dishes - Alyssa, Emily, Jon

Plant species composition transect data entry - Slater, Ainsley, Brian

Sieve LTER T3, T4 soil samples -

Plant species composition transect data entry -

Plant species composition transect data entry - Brian

GC Sequence LTAR20250918S24BGC had vials submitted out of order. All vials run in sequential order, but these two vials were out of order as submitted: 34 and 33.

BCSE gas vial, tote prep - Stacey

Finish sorting SF1 ANPP - Olivia, Sarah, Emily

Weigh LTAR wheat - Brian, Stacey

Sieve and package LTER Resource Gradient soil to send to A&L. - Alyssa, Ainsley, Emily, Jon

Weigh LTER wheat - Slater

WPS training - Brian

Lab clean-up, dishes - Brian

Plant species composition transect data entry - Ainsley

Weigh LTER wheat - Brian

Sort SF1 ANPP samples -

GC sequence LTER20250819S12BGC had some vial order issues as noted from techs. All vials run in sequential order on GC. Note from techs that vials 185 and 186 were swapped and that vials 145-153 were swapped with vials 173-181, as noted on the data sheet.

GC Sequence LTER20250911S13BGC had a few vials submitted out of order. All vials were run in sequential order on the GC. Vials submitted out of order as follows: …201, 202, 204, 203, 205, 206… and then …238, 239, 252, 241, 242, 243, 248, 245, 246, 247, 244, 249, 250, 251, 240, 253, 254, etc.

GC sequence LTAR202508210903S22S23BGC had a few vials submitted out of order. All samples run in numerical sequence on GC. These vials were out of order for Series 22: …112, 115, 113, 114, 116, 117…

Finish sorting T6 ANPP - Stacey, Alyssa, Jon

Sort T6 ANPP samples - Trajana

Sort SF2, SF3 ANPP samples - Stacey

Weigh LTER wheat - Slater, Trajana

LTAR gas vial, tote prep - Ainsley

Filter LTER soil extracts - Jon, Ainsley

Acid washed all Channel 2 reagent containers, dishes.

Prepared 1M KCL.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Weigh LTER soil for extraction and moisture - Slater, Ainsley

Measure and filter leachate, dishes - Jon, Emily

Weigh LTER wheat samples - Trajana

Sort T6 ANPP - Brian

Sieved LTER soil samples -

Transect data entry - Trajana, Ainsley, Stacey

Sort T6 plants - Alyssa, Jon, Ainsley

BCSE gas vial and tote prep, sort T6R1S2,3 plants to evaluate switch from scissors to electric trimmers = small plants still in good shape, no significant difference in quality of cut samples, will use electric trimmers going forward. - Stacey

LTER gas vial and tote prep, transect data entry - Alyssa

Sort T6 plants - Stacey, Trajana

Filter LTAR soil extracts - Ainsley, Sarah

Prepared 1M KCL

Weigh LTAR soil for extraction and moisture - Alyssa, Jon

Filter LTER leachate, dishes - Brian, Ainsley

Sort plants - Jon

Sort T7 plants - Alyssa, Ainsley, Stacey, Slater, Emily, Brian

Dishes - Jon, Trajana

Grind 2024 LTAR fields plant samples - Sarah

Sort T7 plants - Stacey, Ainsley, Emily, Trajana

Sort T7 plants - Emily, Ainsley

GC run sequence Blesh-UDF20250825S26.BGC had the following issues:

1. vials all run in sequence, but 2 vials submitted in rack out of order - vial 55 and 56 were reversed in position when received from field
2. Visual computer signal lost on run startup, but data seemed to be collecting fine. Leter found data stopped being collected at about sample/std vial 19 or so. Rebooted computer to restart run the next morning.
3. Following morning discovered the Licor box detector was shot, throwing wild erroneous peaks and valleys. Box was replaced with AnnaGC box. Restarted sequence near where it left off, with a new “B” sequence.
4. No peaks were seen on “B” sequence. Stopped and restarted. Autosampler proceeded to bend the injection needle like an accordion, before even attempting to draw sample. So, shut everything down to investigate.
5. Discovered nitrogen port on syringe had been sheared off. Replaced needle and syringe and rebooted everything - GC, Autosampler, and Computer.
6. Deleted “B” run due to null data and restarted “B” run all over again. Everything seems to be working now…

Sort T7 plants - Trajana, Sarah, Ainsley

Sort T7 ANPP - Ainsley, Emily, Stacey, Slater, Jon, Trajana

Sort T7 ANPP -

BCSE gas vial, tote prep - Ainsley

Sort T7 ANPP - Stacey

Sort T7 ANPP - Stacey, Olivia, Sarah, Trajana

Sort ASP5 plants -

Measure and filter LTAR leachate - Ainsley, Slater

Filter LTER soil extracts -

Weigh LTER soil for moisture and N-extraction - Sarah, Brian

Sort ASP2 plants - Alyssa, Olivia, Emily, Jon, Trajana, Ainsley, Slater, Brian

Dishes - Sarah

Prepared 1M KCL

LTAR202507170724S18S19BGC sequence had a vial order issue. There were vials submitted out of order for Series 18 such that I ran them in sequence 31, 32, 36, 33, 34, 35, 37, 39, 38, 40, 41 for Round “A,” but then forgot to make the same change for Round “B.” So Round B was entered in the sequence as running in consecutive order (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, etc.). Please refer to data sheets for clarification on this. I will change the Round “B” labels in the Excel results file to properly match Round “A.”

Sieve LTER soil - Alyssa, Ainsley, Sarah

LTAR gas vial and tote prep - Trajana

Filter soil extracts, dishes - Brian, Ainsley, Sarah

Sort plants - Stacey

Weigh T21 incubated soil from T3, T4 stations and prairie strips - Sarah, Ainsley, Emily

Dishes - Jon, Ainsley

LTER gas vial and tote prep - Olivia

Sort plants - Alyssa, Stacey, Olivia, Trajana

LTAR202507310807S20S21BGC sequence had vials submitted out of order. All vials run on GC in consecutive order. Vials out order as follows: …125, 126, 131, 132, 129, 130, 127, 128, 133, 134…. Also “Dup” vials were all submitted upside down in sampling rack. Could mean they weren’t sampled?

BCSE2025072507310814S15S16S17BGC GC sequence had vials submitted out of order. All vials run on GC in consecutive order. Vials that were out of order were as follows: S16 - …16, 17, 19, 18, 20, 21… and vial #75 and vial #Dup3 were switched for each other; S17 vials in order except as noted here - …60, 61, 78, 79, 80, 65, 74, 75, 76, 69, 70, 71, 72, 73, 66, 67, 68, 77, 62, 63, 64.

Sort plants -

Filter leachate, filter LTAR soil extracts, dishes -

Weigh LTAR soil for extraction and moisture - Brian, Ainsley

Sort plants - Brian, Emily, Ainsley, Sam, Trajana, Slater, Jon

Sieve LTAR soil -

Empty FSC and FL ovens -

BCSE vial and tote prep, weigh dry tins, dishes - Ainsley

Sort plants -

Filter soil extracts -

Sort plants -

Weigh LTER T21 soil for extraction and moisture - Ainsley, Sarah

Finish grinding 2024 LTAR plants - Sam

Empty Grieve oven, dishes, weigh tins -

Sort T3, T4 prairie strip ANPP -

Dump leftover/extra LTAR plant material -

Grind plants -

Sort plants - Alyssa, Ainsley, Emily, Sam, Brian, Slater

Filter soil extracts, dishes - Olivia, Sarah, Jon

Weigh LTER soil for extraction, moisture - Brian, Sarah

Grind plants -

Finish sorting SBE ANPP, then sort ASP6 - Stacey, Alyssa, Trajana, Jon, Sam, Olivia, Sarah

Grind plants -

LTAR gas vial, tote prep - Trajana

Sort SBE ANPP -

Sort SBE ANPP samples - Alyssa, Ainsley, Emily, Trajana, Sarah, Olivia, Stacey

Grind plants - Jon, Sam

Finish BAF deep core soil subsampling, weigh soil tins - Sam

Dishes, soil sampling prep - Brian, Jon

Finish sorting T8 plants - Alyssa, Ainsley

Sorted SBE ANPP plants to species - Stacey, Brian, Sarah, Blackwood lab (2)

Grind plants - Sarah, Sam

BAF subsampling for 2022 BCSE deep core soil - Jon

LTER field lab clean-up - Stacey, Brian, Sarah

Sort plants - Alyssa, Stacey

Sort T8 plants - Alyssa, Slater, Emily, Ainsley

Dishes - Olivia, Jon

LTER gas vial, tote prep - Sarah

Grind plants, pulverize deep core soil (BCSE 2022 finished) - Sam, Stacey

Sieve LTER Extensive Grid soil and remove subsamples to send to A&L for S1 test package -

Sort T8 ANPP plant samples - Alyssa, Ainsley, Emily, Trajana, Sarah

BCSE and LTAR gas vial and tote prep - Stacey

Measure and filter LTAR leachate, dishes - Ainsley, Emily, Alyssa trained

WPS Zoom training - Ainsley

Finish sorting T5 plants, start sorting T8 ANPP samples -

Grind plants and pulverize soil -

Sort T5 plants -

GC run LTAR202507030710S16S17BGC (and version “B”) notes: Analysis setup and computer froze overnight, prior to completing first set of standards - i.e. system ran through all methane analyses, but only recorded results through standard vial 13 or so. Therefore, the system was rebooted and rerun at that point. At rerun, initial few standards looked to be contaminated - may use initial run for 1st standard set and “B” run for sample and final standard results. Sampling notes - series 17 vials submitted with note that vials 141 and 117 were swapped. These were run in swapped order on the GC (i.e. 116, 141, 118.. then 140, 117, 142… etc.) Additionally, sample vial 93 from series 17 was dropped and broke. The vial was replaced in the run, but just contained lab air.

Sort T5 ANPP -

Filter LTER T3, T4 soil extracts -

Empty drying ovens -

Prepared 1M KCL

Weigh T3, T4 soil for extraction, moisture, and T21 whirlpaks for incubation - Alyssa, Ainsley, Emily, Brian, Jon

Sieve T3, T4 soil - Alyssa, Ainsley, Emily, Trajana

Measure and filter LTER leachate - Sam, Trajana

Grind plants and pulverize BCSE deep core soil -

Robertson lab site-specific training - Stacey with Ainsley and Emily

WPS site-specific training - Brook with Ainsley and Emily

Finish sorting T4R6 plants from wheat sampling, dishes, weigh dry soil tins, clean lab, dump soil buckets - Brian, Jon, Olivia, Sam, Trajana, Emily

Onboarding - Ainsley

LTAR gas vial prep - Stacey

Dishes, WPS training - Emily

Filter LTER soil extracts - Emily (Stacey trained), Alyssa

BCSE gas vial prep - Ainsley

LTAR and BCSE tote prep - Stacey

Lachat run Inhibitor Soil N Content (Clara).

Prepared 1M KCL.

Weigh LTER soil for extraction, moisture and T21 incubation - Brian, Sam, Jon, Sarah pm

Measure and filter LTAR leachate - Alyssa, Emily, Ainsley

Onboarding - Emily, Ainsley

LTER gas vial prep - Ainsley

Sieve LTER soil samples - Sam, Slater, Brian, Sarah, Jon

Dishes, data entry - Olivia, Trajana, Jon

Finish sorting ASP2 forage samples - Brian, Slater, Sarah, Sam

Filter LTAR soil extracts -

Weigh LTAR soil for extraction and moisture - Brian, Sam

Sort ASP2 forage samples from 6/30 - Stacey, Leah V

Prepared Standards, prepared 1M KCL.

Prepared hand dilution standards - Dilutor of Lachat is not functioning and was sent in for repairs.

Sieve LTAR soil -

BCSE and LTAR gas vial prep, weigh dry BCSE tins, dishes - Stacey

GC sequence LTAR202506190626S14S15BGC tech notes: Series 15 vials 140 and 144 were empty, contain no sample. They were run in place, so will just appear to be air samples…

Sequence LTER20250620S8BGC issues: All GC vials run sequentially, some samples submitted out of order in trays. Vials 41 and 57 were swapped (i.e. …39, 40, 57, 42, 43… and …55, 56, 41, 58, 59…) One other set of vials submitted out of order as follows: …105, 106, 111, 108, 109, 110, 107, 112, 113…

BCSE20250530060506130619S7S8S9S10BGC sequence had one exception: Series 10 had a note that vials 8 and 5 were swapped in the field. Therefore, for series 10, they were run in that order on the GC, i.e. …3, 4, 8, 6, 7, 5, 9, 10….

BCSE gas vial, tote prep - Sarah

Grind plants, pulverize soil - Sam,

Archive air-dried soil -

Pack CN - Brian

Data entry -

Grind plants, pulverize soil - Olivia, Jon,

LTAR gas vial, tote prep, pack CN - Olivia

Filter BCSE soil extracts -

Archive air-dried soil - Slater

Dishes -

BAF subsampling - Trajana

Sort ASP6 R5 plants to species - Slater, Brian, Trajana

Weigh dry tins, BCSE soil for extraction and moisture - Trajana, Olivia

Grind plants, pulverize soil - Sara, Olivia

Dishes - Jon

Pack CN - Brian

Acid washed dishes, Prepared 1M KCL.

Lachat run LTER Soil 20250324 - T6 not sampled, Samples from T8R4 a-c were not filtered, run as blanks.

Grind plants and pulverize soil -

Dishes -

LTER gas vial, tote prep - Brian, Sarah

Grind plants, pulverize soil - Sam,

Dishes -

Measure and filter LTER leachate -

Filter LTER extracts -

Dishes -

Acid washed dishes.

Weigh LTER soil for extraction and moisture - Sam, Jon

Grind plants, pulverize soil -

LTAR, BCSE gas vial prep -

Dishes

Prepared Standards, reconditioned cadmium column. Prepared 1M KCL.

Lachat run LTAR lysimeter 20241209

• Samples BAU2 R2 N and ASP2 R3 C had vials filtered, but had volume listed as 15ml, ran samples as vials had enough sample for 3 samples and most likely had a volume at or above 20ml.
• Sample ASP2 R4 S had no volume listed, ran as missing vial ASP2 R4 C.

Finished all 2024 Lysimeter samples - LTAR files not uploaded.

Sieve LTER soil -

Acid washed dishes.

Grind plants, pulverize soil - Trajana, Slater, Sam

Dishes - Jon, Olivia, Trajana

Grind plants -

BCSE gas vial, tote prep -

Pulverize soil -

Pack CN, dishes, data, BAF subsampling -

GC Sequence LTER20250606S7BGC had just a few samples submitted out of order: All vials run in numerical sequence on GC. Submitted vials out of order in trays: …232, 234, 233, 235, 236….

Measure and filter leachate - Sarah, Jon

Dishes - Sam

LTAR gas vial, tote prep - Olivia, Trajana

Filter LTAR soil extracts - Sarah, Jon

Grind plants, pulverize soil - Trajana, Olivia

Lachat run LTAR Lysimeter 20240812. -Vial ASP1 R4 N has no estimated volume listed in data sheet, could be missing vial BAU2 R4 S? Potentially labeled incorrectly.

Prepared 1M KCL

Weigh LTAR soil for extraction and moisture - Sarah, Slater

Grind plants, pulverize soil, archive air-dried soil, dishes, data, BAF subsampling -