Projectlog Entries List

Finish sieving and subsampling GLBRC SVII soil. Finish relocating soil archive for construction. - Emily, Jon, Glori

LTAR gas chamber prep - Emily, Glori, Jon

Sampled soil from Switchgrass Variety II (SVII) experiment, five 0-25cm cores pooled per split plot, 72 samples total - Emily, Glori, Jon

Weigh LTAR plot R3,4 0-10cm soil into 50mL centrifuge tubes for microbial CN. - Jon, Stacey

Sieve GLBRC SVII soil and subsample for A&L. - Jon, Glori, Emily

Extract and filter LTAR R3,4 unfumigated soil for microbial CN. Add chloroform to fumigated soils. Dishes - Stacey

LTAR gas vial prep - Alyssa, Stacey

Weigh LTAR plot R1,2 0-10cm soil into 50mL centrifuge tubes for microbial CN. Dishes - Jordyn, Stacey

Sieve LTAR R3,4 0-10cm soil from Sprunger lab. Sieve SBE soil and subsample for A&L. - Jon, Glori, Jordyn

Extract and filter LTAR R1,2 unfumigated soil for microbial CN - Stacey

Dishes - Jon

Weigh LTAR and MLE Lux Arbor dry soil tins - Alyssa

Sieve LTAR R1,2 0-10cm soil from Sprunger lab. Continue to relocate soil archive samples. - Glori, Emily, Jon

Temporarily relocate soil archive from the field lab to P.B. wash bay during field lab construction - Jon, Emily, Glori, Jordyn, Alyssa, Stacey

Dishes - Jon

Filter MLE Lux Arbor soil extracts - Jon, Glori

Weigh MLE Lux Arbor soil for extraction and moisture - Emily, Glori

Dishes - Jon, Glori

Sieve MLE Lux Arbor soil - Emily, Glori, Jordyn

Filter LTAR soil extracts, dishes, weigh dry MLE Lake City soil tins - Jon, Glori, Jordyn, Emily

Finish sieving LTAR plot soil - Alyssa, Emily

Weigh LTAR soil for extraction and moisture - Jon, Glori

Weigh dry MLE Escanaba soil tins - Stacey

LTAR gas vial, tote prep -

Sieve LTAR soil, remove subsamples for A&L S1 analysis from main plot soil only (not noNI microplots) - Alyssa, Emily, Jon, Glori

Filter MLE Lake City soil extracts - Jon, Glori

Weigh MLE Lake City soil for extraction and moisture - Jordyn, Glori

Filter MLE Escanaba soil extracts - Emily, Jon

Weigh MLE Escanaba soil for extraction and moisture -

Sieve MLE MN Escanaba (sampled 10/13/2026) and MC Lake City (sampled 10/14/2026) soil samples - Alyssa, Glori, Emily, Jon

LTER field lab clean-up - Stacey

BCSE gas vial, tote prep - Glori

Filter MLE Rhinelander soil extracts - Glori, Alyssa

Weigh MLE Rhinelander soil for extraction and moisture - Alyssa, Jordyn

Filter MLE Hancock soil extracts - Jon, Emily

Weigh MLE Hancock soil for extraction and moisture , dishes - Jon, Emily, Glori

Finish MLE Rhinelander soil sieving - Alyssa, Olivia

Dishes - Alyssa

NAS data entry - Olivia

Sieve Hancock (10/6/2026) and Rhinelander (10/7/2026) soil. Sampled by Chris and Todd. - Emily, Glori, Alyssa

Weigh dry soil tins, dishes - Stacey

Hand biomass sampled WCG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with hedge clippers, and placed in burlap bag to be dried then weighed.

Weigh G2 sorghum ANPP samples before putting into the oven - Glori, Emily, Jordyn, Olivia, Alyssa

Lab clean-up, update ChemInventory - Alyssa, Stacey

Weigh dry LTAR soil tins - Stacey

DawnGC run notes: BCSE Series 16 - vial 12 flipped upside down in sample tray. Guessing this means there was no sample taken, but vial flipped upright and analyzed to maintain continuity throughout the runs.

Weigh dry LTER soil moisture tins, start checking GLBRC transect data (Sven scanned) - Stacey

ANPP Sampling Lux Arbor MLE site

Personnel: Chris Robbins and Todd Martin

LTAR, BCSE gas vial and tote pre - Olivia, Jordyn, Stacey

ANPP Sampling MLE site Escanaba MI

Personnel: Chris Robbins and Todd Martin

Plant Sampling of Switchgrass P&K Study. Two samples per plot (1+N, 1 0N) Sample taken from pre-determined 1 sqft. area within each side of the plots. Switchgrass plants harvested at ground level, bagged. Samples taken to the Agronomy Farm to be dried and processed to be sent for analysis later.

Personnel: Chris Robbins, Todd Martin

Finish sorting T6 ANPP -

BCSE gas vial, tote prep -

Pack LTAR 2024 plants CN - Jordyn

BCSE S13 S14 S15 GC run had some samples submitted out of order.
S14 had vials 19 and 20 switched in the field racks. S15 had four out of order submitted as follows: vial 52, 50, 51, 49 All samples run sequentially on DawnGC

ANPP Sampling at MLE sites Hancock, Rhinelander.

Personnel: Chris Robbins and Todd Martin

ANPP Sampling at Lake City MLE site.

Personnel: Chris Robbins, Todd Martin

removed the logger, tr100, CH150, SDM8x50 and battery from the transitional site at the fence. The logger box and the TDR cables are still in the box. The SDM8x50 wing devices are also still at the site. I did not check what is still in the forest end box at the transitional site.

BCSE gas vial and tote prep - Stacey

Pool T21 soil bags A and B from T3, T4 stations and prairie strips -

Lachat runs GLBRC soil 20260424 and 20260622.

Plant Sampling of Switchgrass P&K Study. Two samples per plot (1+N, 1 0N) Sample taken from pre-determined 1 sqft. area within each side of the plots. Switchgrass plants harvested at ground level, bagged. Samples taken to the Agronomy Farm to be dried and processed to be sent for analysis later.

Personnel: Chris Robbins

Measure and filter LTAR leachate, filter LTER soil extracts, LTAR and BCSE gas vial prep - Jordyn, Glori, Emily, Brian

Sort ASP1 forage - Sam, Jon

Weigh T3, T4 soil for extraction, moisture and T21 whirlpaks (90-100g/whirlpak) - Olivia, Brian, Jordyn

BCSE gas tote prep, dishes - Jordyn, Brian

Dishes - Jordyn

BCSE new vial prep - Glori

Field lab clean-up - Brian

LTAR gas chamber prep - Brian, Jon, Sam

Sticky traps - Brian, Emily

Collect LTAR leachate - Sam, Jon

BCSE 11 gas sampling and Hydrosense - Emily, Glori

Weigh LTER soil for extraction, moisture and T21 incubation - Glori, Jordyn, Brian around 11

BCSE and LTAR gas vial prep - Jordyn

BCSE gas chamber prep, then evacuate LTAR lysimeters - Emily, Brian

Sticky traps - Jon, Sam, Emily

Bury T21 soil bags. Bury whirl-paks in their corresponding plot. Measure or pace ~7m in a NE direction from the SW corner of each plot. At that point, place a meterstick perpendicular to the tape measure at the 50cm mark on the meterstick. Bury T21 A at one end and T21 B at the opposite end. In corn and poplar plots, the spacing should be adjusted to ensure both bags are buried within a row of wheat or poplars. Each bag gets buried over an orange garden marker at about a 45-degree angle with the top of the bag about 1” below the surface and the marker sticking out of the ground 1-2”. For removal in 21 days - someone can pull up on the orange marker to easily remove the bags. For burial, use a trowel or narrow shovel to lift the soil, place the marker and bag, and then remove the trowel and press down the soil to ensure contact with the buried bag. Try to disturb the plants as little as possible. A flag is placed at the 50cm point midway between the buried bags to help in locating them later. In T1-4 the flag should be in a corn row between the buried bags with all bags and flags within a row of corn. Wherever helpful, tie flagging tape on a plant above the flag, so it is easily located for collection.

• Emily, Brian, Jon, Sam, Glori, Stacey

Weigh and filter LTER leachate - Olivia, Jordyn

Filter LTAR and BCSE soil extracts - Glori, Brian

Weigh LTAR and BCSE soil for extraction and moisture - Glori, Jordyn

BCSE gas vial, tote prep - Glori

Grind LTAR plants - Brian

Pack CN - Jordyn

Grind plants - Glori, Sam

Sieve LTAR and BCSE soil -

Pack CN - Olivia

Archive LTER air-dried soil -

Measure and filter LTAR leachate - Brian, Jordyn

Filter LTER soil extracts - Jon, Glori

Pack CN - Jon

Grind LTER 2026 plants - Sam

BCSE gas vial, tote prep - Glori

raised the antenna on the Switchgrass tower at lux arbor to improve connectivity.

Measure and filter LTER leachate -

BCSE gas vial and tote prep -

Filter NAS soil extracts for Olivia -

Air Temperature sensor at the Hancock Weather station failed. It's returning -100 C. and 112 logger is having trouble sending data.

I stopped the program to help with the download but it had no effect. I will restart the program.if I can't get it to download and check in a few days. The status table can be read but the status summary is empty.

power cycled cell modem at Lux Arbor to restore connection. Adjusted solar panels to the summer position.

Sort ASP1 plants - Olivia am/pm

Measure and filter LTAR leachate - Jordyn, Glori

Filter LTER Soil extracts - Emily, Bella

Prep BCSE gas vials, totes - Emily, Bella

cleaned out raingauge on the BCSE

Noticed that the funnel was off on the rain gauge. It might have been blown off with the recent wind storms. I re-installed the funnel

BCSE Series 1 2026 gas sampling - Jon, Olivia

Adjusted REX and BCSE Solar panels to winter position.

BCSE2025072507310814S15S16S17BGC GC sequence had vials submitted out of order. All vials run on GC in consecutive order. Vials that were out of order were as follows: S16 - ...16, 17, 19, 18, 20, 21... and vial #75 and vial #Dup3 were switched for each other; S17 vials in order except as noted here - ...60, 61, 78, 79, 80, 65, 74, 75, 76, 69, 70, 71, 72, 73, 66, 67, 68, 77, 62, 63, 64.

BCSE20250530060506130619S7S8S9S10BGC sequence had one exception: Series 10 had a note that vials 8 and 5 were swapped in the field. Therefore, for series 10, they were run in that order on the GC, i.e. ...3, 4, 8, 6, 7, 5, 9, 10....

Trenched in power for M2,3,4 and L1,2 today. At the M4 reference, was only able to trench maybe 6inches. KK/DW

BCSE202410071104S16S17BGC run had some submitted samples out of order. All samples run on GC in numerical order. Series 16 samples out of order, submitted in following sequence: ...10, 11, 16, 13, 14, 15, 20, 17, 18, 19, 12, check, 21, 22....

Lux marginal land site visited by Kevin/Stacey today. Found many corner posts and sockets pulled out and also north side of G10R3 found soil moisture probes dug up and chewed. Will not be able to repair. They are soil moisture profiler sensors for this plot..

KK/SV finished switchgrass gradient deep cores today, 6 plots (12 cores). Total of 64, two per plot. Core 1 was always in the south half of the plot and Core 2 in the north.

Completed all but 12 deep cores on the Switchgrass Gradient project. KK/DW Still need to collect inorganic N samples and another gas sample due soon.

Deep core of Switchgrass N Rate trial started today. KK/DW We cored the western 6 plots, 12 cores just after strip harvest.

Removed all chambers from SWFG study for harvest.

Adjusted all chamber heights in gashog to reflect reinstalled ht.

Removed G5 autochambers for microplot harvest in the afternoon. Turned off chambers on gashog.

GC Run SWFG series39 10/8/2024 2 vials out of order at sampling: 14 and 15 were switched at sampling, so will be run in the order ...12, 13, 15, 14, 16, 17.... GC Run SWFG series41 10/23/2024 2 vials out of order at sampling: 93 and 94 were switched at sampling, so will be run in the order ...92, 94, 93, 95, 96....

Dave and Kevin replaced the chambers on G1 and restarted sampling. G1-3 overextending. Raised all tubing off the ground. Chambers removed 10/22 pm

Checked all chambers in G1 and G5, except G5-5 (closed at the time). Trimmed corn leaves out of the path of the closures on G1 chambers. Most G5s were in good shape and required minimal trimming on this visit. All G5 lid connections appeared to be intact and working properly.

BCSE202407190731S12S13BGC: All vials run in consecutive order. Vials submitted by techs in this order for Series 12: 1, 18, 3, 4, 5, 14, 7, 8.... ....12, 13, 6, 15, 16, 17, 2, 19, 20, 21.... all other vials in order.

G5-3 chamber lid fixed. May have been okay up to point of checking lids, but arm attachment popped off when checking. Fixed.

I removed the radio, datalogger, TDR100, relay, battery and antenna from the DF site.

Running 3 series of GLBRC gas samples. Notes on Series 6 from Techs - Vials 1-40 swapped, i.e. vials 1-20 are actually vials 21-40 regarding site sampling. Series 7 no notes. Series 8 - a number of vials out of order. All samples run in numerical order. Series 8 vial order in trays: 1-40 ok, then 41, 42, 59, 60, 45, 46, 55, 56, 49, 50, 51, 52, 53, 54, 47, 48, 57, 58, 43, 44, 61, 62, 63,...

Removing G1 lids for herbicide today. 4 Hr REI.

Approximately 4pm, trimmed switchgrass and any other plant material growing out of the G5 automated chambers. Also checked lids and trimmed any plants outside of chambers that were interfering with lid seals.

GLBRC Series 4 vials. Note from techs that vials 1 and 21 were swapped (i.e. 21, 2, 3, 4, 5...20, 1, 22, 23, etc.). Still run on GC in consecutive order (1, 2, 3, etc...)

It appears the ICOS needs to have the mirrors cleaned. Likely some water entered the system over the weekend. Caliubration worked but sensitivity is down, and noise up.

Trailer went down over the weekend. ICOS concentrations way off and flow blocked. Found issue with valves, then recalibrated ICOS with our reference gas. Added lids back on G1 last Friday. Currently not running trailer until morning.

Removed chambers on the SWFG for fertilization. Will install tomorrow and clip in AM, then sample after lunch.

G5-3 updated chamber depths after moving: corner heights - 32.5cm, 32.2cm, 31.0cm, 30.0cm; center height - 29.5cm.

Moved chamber 5-3 approximately 1 meter to the west to encompass some switchgrass plants, approximately 2pm. Trimmed all chambers in G5. Replaced clamp head on G1-5 (previous one had popped off a couple times).

G1-6 showing negative CO2. Will check. Also removing chambers on SWFG for fertilization today.

BCSE automated chamber notes: G1-5: discovered a missing nut/bolt combo on lid that may leak air. Plugged temporarily with weatherstripping on both sides of hole. G5-1, 5-3, 5-4 chambers have very little to no switchgrass growth inside the chambers. May be time to move them to be more representative of the plot. G5-2: switchgrass growth to a few inches above top of chamber. This was trimmed back to being even/slightly below top of chamber.

Re-installed G1 chambers after planting and liming management earlier in the day. Chambers all had to be shifted somewhat east to line up between newly planted rows. Four corner average heights recorded, along with one center measurement. (All numbers in centimeters.) G1-1: 30.7, 30.5, 29.7, 30.1cm, 30.5 center G1-3: 15.5, 15.6, 15.5, 15.6cm, 15.7cm center G1-2: 29.8, 30.1, 28.4, 28cm, 29cm center G1-6: 29.2, 28.9, 29.3, 29.5cm, 30cm center G1-5: 30, 29.8, 29.2, 28.9cm, 29.5cm center G1-4: 29.3, 29.5, 27.8, 28.2cm, 29.5cm center

G1 chambers flagged and removed for planting and liming today. Chambers all removed by 9:30am. Will replace when farm management notifies us they have completed their work.

Checked automated chambers. No issues seen with 1-6, though reports of issues from remote data. 5-4 was missing a clamp head. Found it and replaced it. May need a new one as a long-term fix.

Disconnected the incoming and outgoing sampling lines from the Licor and ICOS in the trailer until we can clear the chambers and get them operational.

G5-6, G1-3, G1-5 all overextended. Turned down compressor outgoing pressure to just over 52psi. Will shut down system today prior to snowstorm and incoming polar vortex.

Found chamber G5-6 (control) had a hyperextended lid on the east side. Put back in place. Going back out to see if I can adjust it with a screwdriver to prevent it from recurring.

Process BCSE deep cores - Sidney, Alex

Process BCSE deep cores - Sidney, Alex

Process BCSE deep cores - Sidney

Drone flight for albedo on BCSE and LTAR. Sunny, light breeze. White tarp measure after each. While flying the LTAR, flight lasted unusually long, when it was returning for next battery at 18%, The drone lost complete power about one foot above the home position. Hit the ground and disturbed the mount for the front camera and the ribbon cord that runs from gimbel to the drone system. Repaired.

G6 ANPP sampling and species composition transects. Three NS transects per plot, main only. Each transect ~2m east of the station flags and numbered 1-3 based on the station number. Stop about every 1m to identify and measure first intercepted plant. ANPP: Station 1 only: 3mS, 0.5mW to NE corner of 1.52x0.66m frame to avoid microplot. All other stations: 3mS, 1.5mW to NE corner of 1.52x0.66m frame. MISGI bagged separately, weeds (if any) sorted to species. - Alyssa, Gloria, Helen, Emily

Process BCSE deep cores - Sidney

Process BCSE deep cores - Sidney

Process BCSE deep cores - Sidney

Process BCSE deep cores - Sidney

Removed flags and chambers from Martha's study on the north baitcrop area BCSE.

Additionally, removed all shelters on the Lux Arbor marginal land site. Three reps show high stress. Also, deer pellets in three of the four sheltered areas. ALOT! Only deployed one set of RH/T sensors in rep four this year.

Process BCSE deep cores - Sidney