Projectlog Entries List

BCSE Series 16 gas sampling - Glori, Jordyn

BCSE Hydrosense - Olivia

Set forest leaf litter traps - Emily, Jon,

Collect T5 LFLIT, trim under and around traps -

Pack LTAR 2024 plants and LTER 2024 RR CN - Bella, Jordyn

Weigh LTER 2026 plants - Olivia

Finish sorting T6 ANPP -

BCSE gas vial, tote prep -

Pack LTAR 2024 plants CN -

BCSE gas chamber prep -

DawnGC sequence LTAR-S21 had a few samples submitted out of order in field trays. Order submitted as follows: ....82, 83, 88, 85, 86, 87, 84, 89, 90,.... All samples run on DawnGC in sequential vial order.

BCSE S13 S14 S15 GC run had some samples submitted out of order.
S14 had vials 19 and 20 switched in the field racks. S15 had four out of order submitted as follows: vial 52, 50, 51, 49 All samples run sequentially on DawnGC

Sort T6 ANPP -

Pack LTAR 2024 plants CN - Olivia

LTAR Series 22 gas sampling -

LTAR Hydrosense -

Clip T6 ANPP -

LTAR gas chamber prep - Jon, Emily

Clip T6 R1, R2 ANPP. 2mS, 2mE to NW corner of 2 x 0.5m frame - Olivia, Jordyn, Glori, Emily

LTAR gas vial, tote prep -

Finish sorting T7 ANPP - Olivia, Jordyn, Glori, Alyssa, Jon, Stacey

Again changed the tower 1 IRGASON out after Moriz (JC lab) recalibrated. Water density jumped by 5 g/m3. Seems like a lot.

About 10:45 AM today.

Tower 1, 6'8" from ground, 5' from crop. changed irgason from 1370 to SN 1375.

There may be continued issues with the 1375 IRGASON.

Sort T7 ANPP - Alyssa, Glori, Bella, Jordyn, Emily, Jon, Olivia

Pack CN - Jon

Clip T7 R5, R6 ANPP -

Sort T7 ANPP - Alyssa, Glori, Bella, Stacey

Clip T7R3, R4 ANPP - Alyssa, Glori, Bella, Jordyn, Emily, Sam, Jon

replaced sunphotometer.

fixed network stoppage and added sd card on tower 2 and tower 3.

LTER Series 12 gas sampling - Jon, Sam, Emily, Glori, Bella, Jordyn

LTER Hydrosense - Emily

Clip T7R2 ANPP - Alyssa, Glori, Bella, Jordyn

Sort T7 ANPP - Alyssa, Stacey, Glori, Bella, Jordyn

Data entry - Glori

LTER gas vial, tote prep - Stacey, Jordyn

Checked tower 1 HFP sensors. Look fine but sensor G2 not responding properly. High values still.

Checked tower 2 HFP g3 and g3. Also look fine but not reading properly.

Checked tower 3 found G2 HFP and 3L CSI 107 cut. repairing and also need to add an additional CS650.

Finished BCSE G4,5,7,9,10 ANPP sampling - Alyssa, Jon, Emily, Glori, Jordyn, Sam, Bella

LTER gas chamber prep -

Start T7 ANPP sampling, clipped R1. 2mN, 4mE of each station flag to SW corner of 2 x 0.5m frame. Refrigerated and sorted later in the field lab - Alyssa, Jon, Emily, Glori, Sam, Bella

Changed IRGASON because last one was not reading correctly, improper calibration. Swapped around 130 and returned to Moriz with Jiquans lab. They used an empty cylinder to calibrate.

Raised ASP3F1 tower (1) IRGASON to 5' above canopy. Canopy ht of 2'. Total 7'

Finish BCSE G4,5,7,9,10 species composition transects and continue ANPP sampling - Alyssa, Jon, Emily, Glori, Jordyn, Sam

BCSE G4,5,7,9,10 species composition transects and ANPP sampling - Alyssa, Jon, Emily, Glori, Jordyn

Weigh LTER plants - Sam pm

Data entry - Jon pm

Pack CN - Bella pm

BCSE gas Series 15 - Jordyn, Emily am, Hydrosense- Emily

BCSE transects - Alyssa, Jon am

BCSE ANPP - Sam, Bella am

Collect LFLIT from T5 traps, trim under traps - Emily, Jordyn

LTAR gas Series 21 - Alyssa, Sam, Jon, Glori, AM Hydrosense - Emily

BCSE gas, vial prep - Olivia, Jordyn AM

BCSE transects - Alyssa, Olivia pm

Move LTER T7 shelters with Nameer/Mark, BCSE ANPP- Jon, Sam, Emily, Glori, Jordyn pm

Tower 4 BAU1F1 raised to 14'3", crop is approx 9'. About as tall as possible. Reestablished N-S coor on the horizontal bar. 330 PM complete.

GC run on Dawn GC: LTAR, Series 19, 07/23/2026. Two vials submitted out of order - 40 before 39. These were switched to proper order on the GC rack and all vials run sequentially. In addition, there was an issue with LTAR Series 18 being stuck on "acquiring" at the very last standard in it's sequence. This caused an issue with loading the Series 19 run, until the Series 18 was aborted for the last standard, and the run cleared from the Run Queue. I think everything worked out, but there may be no data for that last standard on series 18.

Continue BCSE G4,5,7,9,10 species composition transects - Alyssa, Bella AM/PM

Start BCSE G4,5,7,9,10 ANPP clipping. All stations: 2mS, 1mE to NW corner of 2 x 0.5m frame. Clip with electric trimmers at ground level, bag as UNSRT. Collect SURFL. - Glori, Jordyn PM

BAU Tower 2 (soybeans) raised to 7ft. Soybeans at tower are at a height of 2 ft. Original tower height was 6 ft. Raised to 7 ft. to be 5 ft above canopy, which is approximately 1.5m difference (between tower sensors and top of canopy).

LTAR gas, vial prep - Glori, Jordyn AM

Sticky traps, remove T-posts - Jon, Emily, Sam AM/PM

Start BCSE G4,5,7,9,10 species composition transects. Three NS transects per plot, main only. Each transect ~2m west of the station flags and numbered 1-3 based on the station number. Stop about every 1m to identify and measure first intercepted plant. - Alyssa, Glori, Jordyn PM

LTER Series 8 06/26/2026 GC run had some samples submitted out of order. All samples run in sequential order, but these vials submitted out of order (as listed): ...220, 224, 222, 223, 221, 225....

LTAR Series 18, 07/20/2026 GC run. Two samples submitted out of order. All samples run sequentially. Sample vials 80 and 79 were swapped in racks submitted for analysis (i.e. vials ordered ...76, 77, 78, 80, 79, 81....).

Sticky traps, Remove T-posts - Jon, Olivia, Emily, Sam AM/PM

Sort LTER T3/T4 Prairie Strip ANPP plants - Alyssa, Glori, Jordyn AM/PM

flumes ASP3F2 and BAU2F1 flumes are now covered with plywood. Collection Bottles were installed by Drew last week. ASP1F2 and BAU1F1 are complete and covered as well.

Will start installing autosamplers this week and next.

Dishes - Sam AM, Jon PM

Sort T3/T4 Prairie Strip ANPP - Alyssa, Jon, Glori, Bella, AM, Alyssa, Sam, PM

LTER T3/T4 Prairie Strip ANPP - Emily, Jordyn, Bella, Glori AM/PM

Dishes - Sam, Jon

Sort T3, T4 prairie strip ANPP plants - Sam, Jon, Emily

Clip T3, T4 prairie strip ANPP - Jon, Sam, Emily

LTER 11 gas sampling - Sam, Olivia, Bella, Glori, Jon

LTER Hydrosense - Emily

Sort T3, T4 prairie strip ANPP plants - Alyssa, Olivia, Jon

Sort T3, T4 prairie strip ANPP plants - Alyssa, Glori, Stacey

LTER gas tote prep - Stacey

WPS - Emily

Sticky traps - Sam, Emily

LTER gas chamber prep - Bella, Jon

Clip T3, T4 prairie strip ANPP - Alyssa, Glori, Bella, Jon, Emily, Sam

Sort T3, T4 prairie strip ANPP plants - Alyssa, Brian, Olivia

Filter T3, T4 T21 soil extracts - Glori, Jon, Emily

LTER gas vial prep - Stacey

Tried to sample LTER soil with bucket augers but conditions were very dry and difficult. Decided to postpone soil sampling.

Finished fertilizing N-deposition microplots in DF3 and CF3 - Sam, Emily

Sticky traps - Brian, Jon

Weigh T21 soil from T3, T4 stations and strips - Brian, Bella, Glori

Fertilize N-deposition microplots in all R1 and R2 plus SFR3 - Sam, Emily

Clip ANPP samples from T3, T4 prairie strips - Brian, Bella, Glori, Alyssa

removed the logger, tr100, CH150, SDM8x50 and battery from the transitional site at the fence. The logger box and the TDR cables are still in the box. The SDM8x50 wing devices are also still at the site. I did not check what is still in the forest end box at the transitional site.

Prepared 1M KCL

Finish pooling T21 soil - Alyssa, Glori, Emily

Sort T3, T4 R1 prairie strip ANPP - Sam, Jon, Brian, Emily, Alyssa, Glori, Stacey

BCSE 14 gas sampling - Brian, Alyssa

BCSE Hydrosense - Alyssa

Collect LFLIT from T5 traps, trim under traps - Emily, Glori

Continue clipping T3, T4 R1 prairie strip ANPP - Sam, Jon

MCSE radio network was blinking and not passing data. I rebooted the base station and it cleared up. Noticed a high volume of watchdog errors on T7R4 due to MQTT being enabled but not used. Disabled MQTT and updated OS on T7R4

BCSE gas vial and tote prep - Stacey

Pool T21 soil bags A and B from T3, T4 stations and prairie strips -

LTAR 20 gas sampling - Alyssa, Glori, Sam, Olivia

LTAR Hydrosense - Emily

BCSE gas chamber prep - Jon

Start cutting T3R1 prairie strip ANPP: Along each EW transect (South, Center, North): 2 x 0.5m frame, 5m south of strip center to center of 2m frame side (north side). Cut T4 corn paths to station flags - Jon, Emily

Collect T21 bags from T3, T4 stations and prairie strips -

LTAR gas vial and tote prep - Sam, Jon

Measure then filter LTAR leachate, filter LTAR soil extracts, dishes - Glori, Bella, Sam

Sort ASP6 ANPP plants - Stacey, Alyssa, Jon, Emily

Sticky traps - Emily, Brian, Jon

Collect LTAR leachate - Glori, Bella

LTAR gas chamber prep - Sam, Jon

Acid washed dishes.

Lachat runs GLBRC soil 20260424 and 20260622.

Exchanged IRGASON for calibrated (by JC lab) around noon today. Also moved our original EC100 box back. Current SN of IRGASON is 100010. Raised the sensor to 62" above canopy and 82" above ground.

Changed out CNR4 cables with DW, reconnected to V116. Check tomorrow.

Finish sampling soil from three LTAR plots that couldn't be finished yesterday due to lightning - Bella

Sticky traps - Sam, Jon

Evacuate LTAR lysimeters -

Finish clipping ASP6 R4 ANPP -

Prepared 1M KCL, prepared standards. Acid washed dishes. Lachat run 2026 July LTER T7 soil (Lau lab).

Weigh LTAR soil for extraction and moisture -

Sort ASP6 ANPP plant samples - Stacey, Jon, Sam, Emily, Brian, Bella, Glori, Jordyn

Removed the rest of the REX sensors and box from T1R2. Also removed the sensors from T2R6. I think that all of the non T7 REX sensors and boxes have been decommisisoned.

LTAR soil sampling for July. Too dry for push corers. Used bucket augers and took one 25cm depth sample from each station. When row crop present/harvested, took one in the row, one half way between rows, and one about a quarter of the way from a row to the next. Sampled noNI microplots the same way. -

ASP6 ANPP sampling; R1-4 all stations: 0.5mS, 0.5mE of each station to the NW corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. Finished sampling R1-3 -

Sieve LTAR soil samples -

Sort ASP6 ANPP -

LTER 10 gas sampling - Brian, Glori, JOn, Jordyn, Sam, Emily (Hydrosense too)

Finish ASP4, 5 flagging stations with REACH - Jon

Sort T8 ANPP - Alyssa, Stacey, Jon

Filter LTER T21 soil extracts - Brian, Jordyn

replaced T7R4 logger with the one from T1R2. The sensors are now all on C3 instead of on C1, C3 and C5. I've adjusted the program to take that into account. Shields not connected to ground. There are 3 sensors installed. (g, f, e). Sensor f does not respond. Since we have only 3 sensors and 4 SDI12 ports I wired each sensor to it's own SDI12 port.

Prepared 1M KCl

Sort T8 ANPP plants - Alyssa, Stacey, Jordyn, Jon, Olivia, Bella, Glori, Sam

Weigh LTER T21 soil samples for moisture and extraction - Brian, Bella

LTER gas vial and tote prep. WPS training - Emily

decommissioned the REX logger box and sensors from T2R1.

LTAR 19 gas sampling - Jordyn, Jon, Sam, Olivia

LTAR Hydrosense - Emily

LTER gas chamber prep - Glori, Emily

Replace LTAR station flags (flagging tape in ASP4,5) with REACH - Sam, Jon

Sticky traps - Emily, Sam

Collect LTER leachate - Jon, Jordyn

LTAR gas chamber prep - Brian, Glori

T8 ANPP sampling. All stations: 1mS, 1mW of each station flag to NE corner of 0.5 x 0.5m frame - Alyssa, Brian, Glori

Measure and filter LTER leachate, dishes - Jon, Jordyn

Start sorting T8 ANPP -

Lachat run LTER Lysimeter 20260402. Notes from run:

• Three vials labeled T3 R3 1, 2, and 3 were ran as T5 R3 1, 2, and 3. Flasks for T3 R3 and T5 R3 were swapped when evacuating samples. Weights were adjusted when entering in data file to the correct location, but vials were sampled incorrectly.

Sort T5 ANPP - Brian, Emily, Jordyn, Olivia, Stacey

Sticky traps - Brian, Jon

Evacuate LTER lysimeters - Glori, Sam

Nitrogen Application Study gas sampling, too dry for Hydrosense - Olivia

Collect three SURFL samples forgotten from T5 understory sampling yesterday - Emily, Jordyn

Collect buried LTER T21 bags - Jon, Sam, Glori, Jordyn, Emily, Olivia

Raised tower 2 (BAU north of pond lab) sensors to 168" (14'). Rest N-S.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Set up a set of six CS650 sensors on a CR800 data logger. All sensors connected to C3 control port. These measure a soil moisture profile. Three sensors in between F5 and F6 on Block 5 1 meter in from the North edge.

Address 1 = 12" depth Irrigated Address 2 = 24" depth irrigated Address 3 = 34" depth irrigated

Also installed three sensors on the rainfed side Block 2 in between F6 and F4, one meter in from the north edge.

Address 4 = 12" depth rainfed Address 5 = 24" depth rainfed Address 6 = 36" depth rainfed.

All sensors installed offset from one another so one sensor does not influence another.

Sven will connect radio and program.

LTAR 18 gas sampling - Glori, Brian, Jordyn, Bella

BCSE 13 gas sampling - Jon, Sam

LTAR, BCSE Hydrosense sampling - Emily

T5 understory ANPP sampling, set LFLIT traps. NS trap… 2.4mN, 1.2mW of station 5 to SE corner of trap, frame oriented with short ends on the north/south; label ex. T5R1 NS EW trap… 2.4mS, 1.2mW of station 3 to NE corner of trap, frame oriented with short ends on the east/west; label ex. T5R1 EW. Collect newly fallen poplar leaves within frame as LFLIT (if any). Clip all biomass rooted inside frame with scissors – as close to the ground as possible. Bag as LIVE to sort in the field lab later (store in black bags in fridge). Use electric trimmers to trim back plants outside the frame to keep them from falling over the frame. Leave the frame in place and lay a screen inside it. Push the screen down inside the frame so it rests on the ground. Use clips (at least 6/trap) to attach the screen to the wooden frame on all sides. - Alyssa, Jon, Emily, Sam, Bella, Glori, Jordyn, Brian

LTER Grad Fellow Symposium

Pack CN - Glori

Data entry - Emily, Jon

LTER field lab to do list (cleaning, organization, dump old samples) - Jordyn, Sam, Brian

Postponed field work, including LTAR 18 and BCSE 13 gas sampling, due to hazardous air quality outdoors from Canadian wildfire smoke.

Reinstalled ASP1 gas chambers after forage baling - Sam

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Replaced Cadmium Column, acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopore H2O.

Weigh LTAR plants - Jon, Jordyn

Sort ASP1 forage - Emily, Brian, Bella, Jon, Sam, Olivia

Pack CN - Sam, Bella

Dishes, lab clean-up - Jordyn, Glori

NAS vial prep and data entry - Olivia

Lachat run LTER Lysimeter 20260319. Notes from run:

• Vial labeled T2 R4 1 should not exist, not enough volume listed. Ran as Vial T3 R4 1, that was not present and should exist.
• Vial T CF R2 1 missing from box, not run (no data notes)
• Vial T6 R3 3 present, should not exist. Not enough volume in vial to run.

The usb to serial pigtail between the rpi and the RF407 radio in the BCSE gas trailer appears to have failed. I replaced it with one from the ICOS system.

Postponed LTAR 18 and BCSE 13 gas sampling due to hazardous air quality outdoors from Canadian wildfire smoke. No field work today.

Prepared Standards.

Lachat run LTAR Lysimeter 20260616. Notes from run:

• Vial labeled ASP2 R1 N should not exist, not enough volume. Ran as vial ASP2 R2 N, that should exist and is missing.
• Vial ASP6 R2 S was missing from box and should exist, not run.

Rebury some T3, T4 T21 bags for station 5 as they were buried on the foot path in error, so moved further west between foot path and prairie strip.

Collect LTAR leachate - Emily, Brian

LTAR, BCSE gas chamber prep - Jordyn, Glori

Sticky traps - Jon, Sam

Measure and filter LTAR leachate, filter LTER soil extracts, LTAR and BCSE gas vial prep - Jordyn, Glori, Emily, Brian

Sort ASP1 forage - Sam, Jon

Sieve LTER soil -

Weigh LTER soil for extraction and moisture - Alyssa,

Sort ASP1 forage, empty ovens - Alyssa

Evacuate LTAR lysimeters - Olivia, Brian

Finish LTER soil sampling - Emily, Glori, Jon, Jordyn, Sam

Sticky traps - Brian, Olivia, Emily, Jon, Sam