Projectlog Entries List

Prepared 1M KCL.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Dishes - Jon, Glori

Sieve MLE Lux Arbor soil -

Ran water test for BAU1F1 flume yesterday. Abraham's homemade sampler worked well, it took most of the day however to determine pump issues with the ISCO. Resolved.

We used the large tank on wheels from Brook/Josh, filled, added an adapter from the tank to a garden hose and ran water through the hose to the front edge of the flume. We also added a block to the throat of the flume so the water level would increase faster.

We made need to change the size of the throat on all flumes to the smallest (0.75) flume size. Then adjust the code to calculate discharge.

FLUMES.

Acid washed dishes.

Pulled ground sensors on tower 4 BAU1F1 and found one heat flux plate chewed in pieces. Added sheathing to two sets of cables, dropped the sensor to 2m and added the temperature sensor for the EC100/Iragson. Reinstalled the sensors around 230PM.

Knocked over the remaining corn stalks around the tower, but did not collect the corn.

Collected SD cards on Towers 1-4, downloaded, saved to "ready for manager" and reformatted the cards.

Tower 2, releveled the IRGASON (it was pretty far off).

Replace station flags with Trimble in T1,2,3 - Emily, Jordyn

Help Chris sample MLE Lux soil -

Finish sampling T4 corn ahead of harvest - Alyssa, Jon, Emily, Glori

Filter LTAR soil extracts, dishes, weigh dry MLE Lake City soil tins - Jon, Glori, Jordyn, Emily

LTAR Series 26 gas sampling - Jon, Emily, Glori, Jordyn

LTAR Hydrosense - Emily

Finish sieving LTAR plot soil - Alyssa, Emily

Weigh LTAR soil for extraction and moisture - Jon, Glori

Weigh dry MLE Escanaba soil tins - Stacey

LTAR gas vial, tote prep -

SGPK: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 2 cores per split. placed in a paper bag and put out to air dry

LTAR gas chamber prep - Emily, Alyssa

Collect leaf litter from traps on T5, CF, DF, SF - Alyssa, Emily, Glori, Jon

Hand biomass sampled MCG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

LTAR soil sampling (four 0-25cm cores/station, 12 cores/plot; noNI microplots 9 cores), took extra soil for A&L and archive - Alyssa, Emily, Jon, Glori

Sieve LTAR soil, remove subsamples for A&L S1 analysis from main plot soil only (not noNI microplots) - Alyssa, Emily, Jon, Glori

Filter MLE Lake City soil extracts - Jon, Glori

Prepared 1M KCL.

Finish sampling T3 corn ahead of harvest, start sampling T4 corn - Emily, Jon, Jordyn, Glori

Weigh MLE Lake City soil for extraction and moisture - Jordyn, Glori

Filter MLE Escanaba soil extracts - Emily, Jon

Weigh MLE Escanaba soil for extraction and moisture -

Finish sampling T1, T2 corn, start sampling T3 corn - Alyssa, Glori, Emily, Jon

Prepared 1M KCL

LTER Series 14 gas sampling - Jon, Glori, Jordyn, Emily, Stacey

LTER Hydrosense - Emily

Sieve MLE MN Escanaba (sampled 10/13/2026) and MC Lake City (sampled 10/14/2026) soil samples - Alyssa, Glori, Emily, Jon

LTER field lab clean-up - Stacey

BCSE Series 19 gas sampling - Alyssa, Glori

BCSE Hydrosense - Emily

LTER gas chamber prep - Jon, Emily

LTER T1R5 corn sampling - Alyssa, Glori, Jon, Emily

BCSE gas vial, tote prep - Glori

Filter MLE Rhinelander soil extracts - Glori, Alyssa

BCSE gas chamber prep - Glori

LTER T1, T2 corn sampling - Jon, Emily

Lake City MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Escanaba MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Weigh MLE Rhinelander soil for extraction and moisture - Alyssa, Jordyn

Filter MLE Hancock soil extracts - Jon, Emily

Start sampling LTER corn from T1 and T2. Used 1.52 x 0.66 m frame; 3.04m S and 1.52m E from each station flag to NW frame corner, oriented with the 0.66m ends on the east and west. Perpendicular to corn rows. - Emily (am), Glori, Jordyn (pm)

Hand biomass sampled MNG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

Prepared 1M KCL.

Finish LTAR ASP2, BAU1 corn sampling - Alyssa, Jon, Emily, Glori

Prepared 1M KCL.

Weigh MLE Hancock soil for extraction and moisture , dishes - Jon, Emily, Glori

Reweigh (partially dry) and shred G2 sorghum samples, then weigh shredded - Alyssa, Olivia

Collect leaf litter from T5, CF, DF, and SF traps - Emily, Glori

Sample LTAR corn from ASP2, BAU1 - Emily, Glori

Mark NAS plots - Olivia

Finish MLE Rhinelander soil sieving - Alyssa, Olivia

Dishes - Alyssa

NAS data entry - Olivia

Sieve Hancock (10/6/2026) and Rhinelander (10/7/2026) soil. Sampled by Chris and Todd. - Emily, Glori, Alyssa

Weigh dry soil tins, dishes - Stacey

LTAR Series 25 gas sampling - Glori, Stacey, Emily, Alyssa

LTAR Hydrosense - Alyssa

LTAR gas chamber prep - Emily, Alyssa

Finish G6 ANPP sampling - Olivia, Glori, Emily, Alyssa

Start sampling LTAR corn, 0.66mS, 0.66mE to NW corner of 1.52 x 0.66m frame. Finished ASP2 R1 - Emily, Alyssa

Rhinelander MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Hand biomass sampled WNG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

LTAR gas vial, tote prep. Filter LTER soil extracts. Dishes - Olivia, Glori

Finish G6 plant species composition transects. Three NS transects per plot, main only. Each transect ~2m west of the station flags and numbered 1-3 based on the station number. Stop about every 1m to identify and measure first intercepted plant. G6 miscanthus (MISGI) ANPP plant sampling. Used 1.52 m x 0.66 m frame. All stations: 1.5mS, 1.5mE to NW corner of frame. - Alyssa, Emily, Jordyn, Glori, Olivia

Weigh LTER soil for extraction and moisture - Glori, Olivia

Prepared 1M KCL

Hancock MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Hand biomass sampled WCG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with hedge clippers, and placed in burlap bag to be dried then weighed.

LTER October soil sampling. Plus, T1R1-3 and DF1-3 7cm depth, 20 cores/plot for Alison King. - Alyssa, Emily, Glori, Olivia

Sieve LTER October soil. Remove 200g subsamples for Yang Lin from T1,2,4 R1,2,4,5. - Alyssa, Emily, Glori, Olivia

Collect LTAR leachate - Emily, Olivia

G6 plant species composition transects - Alyssa, Glori

Measure and filter LTAR leachate - Olivia, Emily

Pack LTAR 2024 plant CN - Jordyn, Olivia

Filter LTER T3, T4 soil extracts, dishes - Alyssa, Emily, Glori, Jordyn

Evacuate LTAR lysimeters - Emily, Alyssa

Prepared 1M KCL

Weigh T3, T4 station and prairie strip soil for extraction and moisture - Olivia, Glori, Alyssa

Finish sorting ASP1 forage - Emily, Glori

Pack LTAR 2024 plant CN - Stacey, Alyssa

Finished sampling G2 sorghum ANPP. Sampled T3, T4 station (2 cores/station) and prairie strip (4 cores/strip) soil. - Alyssa, Olivia, Emily, Glori

Retrained Dawn GC gerstel sampler to injector, and tray. Agilent engineer changed entire front board, display and light now functioning.

Lowered tower 1 ASP3F1 back to 2m (6'7") and reinstalled ground sensors. Planted wheat this morning with blue donkey implement, leaves greater area around tower unplanted. Josh sowed seeds around tower this morning.

added IRGASON temp sensor to tower three at 9:30AM. ASP1F1 forage. After last cutting.

Sieve LTER T3, T4 station and prairie strip soil. Sort ASP1 forage - Alyssa, Olivia, Emily, Glori

Raised tower 2 on BAU2F1 soybean around 1130 and removed ground sensors. Found two HFP cut and not really repairable.

Reinstalled ground sensors and lowered tower to 2m at 345PM.

BCSE G2 ANPP sorghum sampling, all stations: 1.5mS, 1.5mE to NW corner of 1.52x0.66m frame - Glori, Emily, Olivia (am), Jordyn, Alyssa (pm)

Acid washed dishes

Weigh G2 sorghum ANPP samples before putting into the oven - Glori, Emily, Jordyn, Olivia, Alyssa

Lab clean-up, update ChemInventory - Alyssa, Stacey

Weigh dry LTAR soil tins - Stacey

DawnGC run notes: LTER Series 12 - duplicate 3 sample vial was empty and vial 84 used a spare vial in its stead. All samples run sequentially on the GC with the spare vial replacing vial 84 and duplicate 3 kept in line as is.

Safety training - Alyssa

ChemInventory updating - Alyssa, Stacey

Dishes - Olivia

Finished GLBRC Scale-up plant species composition transects - Alyssa, Olivia

BCSE G1 ANPP corn sampling, all stations: 1.5mS, 1.5mE to NW corner of 1.52x0.66m frame - Glori, Emily

Tower 1 over ASP3F1 raised to 12.5' at 11:30ish. Ground sensors removed also for soybean harvest. KK

DawnGC run notes: BCSE Series 16 - vial 12 flipped upside down in sample tray. Guessing this means there was no sample taken, but vial flipped upright and analyzed to maintain continuity throughout the runs.

DawnGC run notes: LTAR Series 23, sampled 9/11/2026 - submitted from field with these notes - "vials 50 & 59 swapped, vial 129 broken." Samples all run on GC sequentially, and a dummy vial placed in the stead of vial 129.

DawnGC run notes: NAS Series14, sampled 07/21/2026 - one set of four was submitted out of order as follows, Vial 17, 20, 19, 18. Vials all run sequentially on the GC, so these may need to be resorted, depending on field note references.

Sort ASP1 forage - Olivia

LTER Series 13 gas sampling - Alyssa, Jon, Emily, Jordyn, Glori

LTER Hydrosense - Olivia

GLBRC Scale-up plant species composition transects - Jon, Alyssa

LTAR Series 24 gas sampling - Alyssa, Olivia, Jon, Glori

LTAR Hydrosense - Emily

Collect T5, CF, DF, and SF leaf litter from traps. Trim under T5 screens. - Alyssa, Glori, Olivia, Emily

Sort ASP1 forage - Olivia

Filter LTAR extracts, dishes - Jon, Glori

LTAR, LTER gas vial and tote prep - Stacey

BCSE Series 18 gas sampling - Emily, Glori

BCSE Hydrosense - Alyssa

LTAR and LTER gas chamber prep - Jon, Olivia, Alyssa

GLBRC Scale-up plant species composition transects - Alyssa, Emily

raised tower 3 over ASP forage to 10'. Then added cable protection to all ground sensors. Need to add one 107 Temp yet. At 330 lowered tower back to 2m and then reinstalled all ground sensors with flaging.

Prepared 1M KCL

Weigh LTAR soil for extraction and moisture - Jon, Emily

Filter LTER leachate, dishes - Olivia, Glori

BCSE gas chamber prep, collect LTER leachate - Glori, Jordyn

GLBRC Scale-up plant species composition transects - Alyssa, Olivia

Sample ASP1 forage ahead of cutting. Stations 1, 2, 3: 1.5mS, 0.5mE to NW corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. Refrigerated to sort later to MEDSA, TRFPR, CICSP, DACGL, BRSNA (live volunteer canola, if any), remainder UNSRT. - Alyssa, Emily, Jordyn, Jon

Collected two water samples via end of flume jar method today. ASP3F2 (100mls) and BAU2F1 (500mls). The other two flumes did not have bottles in place. Something moved them a meter or so away.

Checked autosamplers, none sampled.

We installed the last two flumes on field 51 (east end) and field 27-2-4 (north end).

Evacuate LTER lysimeters - Glori, Emily, Jon

LTAR soil sampling - Alyssa, Jon, Emily, Olivia, Glori

Sieve LTAR soil - Alyssa, Olivia

Weigh dry LTER soil moisture tins, start checking GLBRC transect data (Sven scanned) - Stacey

Replace ASP4, ASP5 station flags using REACH - Stacey

LTAR soybean sampling: BAU2 R1-4, 30" row spacing, all stations: 0.66mS, 0.66mE to NW corner of 1.52 x 0.66m frame, oriented with the 0.66m ends on the east and west. 1.52m side perpendicular to soybean rows. ASP3 R1-4, 5" row spacing, all stations: 0.5mS, 0.5mE to NW corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. Perpendicular to soybean rows. - Olivia, Jordyn

ANPP Sampling Lux Arbor MLE site

Personnel: Chris Robbins and Todd Martin

Finish MLE MN - Escanaba plant species composition transects - Alyssa, Jon, Emily, Glori

Filter LTER soil extracts, dishes - Jordyn

MLE WN - Rhinelander and part of MN - Escanaba plant species composition transects - Alyssa, Jon, Emily, Glori

DF1-3 leaf litter trap barrel was cracked where one of the four bolt legs connected, so unstable. Disposed of broken barrel, replaced with new barrel and wooden cradle. - Stacey

Weigh LTER September soil for extraction and moisture - Stacey

MLE WC - Hancock plant species composition transects - Alyssa, Jon, Emily, Glori

LTAR Series 23 gas sampling - Stacey, Emily, Olivia, Jon

BCSE Series 17 gas sampling - Glori, Jordyn

LTAR and BCSE Hydrosense - Emily

Collect leaf litter (T5, CF, DF, SF) - Olivia, Jordyn, Jon, Glori

Sieve LTER soil - Stacey

LTER September soil sampling in T1-7, bucket augers used in T2, T4 and T5 with one sample taken from S1-4 alternating within and between the corn poplar rows. - Alyssa, Jon, Emily, Glori, Jordyn

Sieve LTER soil - Emily, Glori, Olivia

MLE Lake City plant species composition transects - Alyssa, Jon, Emily, Glori

Sample additional SWF soil for Darcy McRose. Sampled ten cores per treatment (SWF1 and SWF8) spread across all replicates to 4-inch depth with 0.75" push corer.  The soil were kept unsieved, in plastic bags and shipped via UPS overnight on ice. - Olivia, Jordyn

LTER forest and T8 soil sampling - Olivia, Jordyn

LTAR and BCSE gas chamber prep - Olivia, Jordyn

Cleaned flumes out today. No water collected. Some animal feces/urine found in ASP1F1. BAU1F1 and ASP1F1 corn showed obvious flow. Evidence being sediment rills in the flume itself.

Samples were collected on 8/4/26 by Brook and Stacey from BAU1F1, BAU2F1, ASP1F1.

DW brought three samples to campus today (above). About 200-300mls each.

We need an SOP for this.

LTAR, BCSE gas vial and tote pre - Olivia, Jordyn, Stacey