Projectlog Entries List

BCSE gas chamber prep - Jon, Emily

BCSE gas vial and tote prep - Glori

Sort ASP1 then ASP5 plants - Jon, Emily, Glori

ASP5 cover crop sampling (end of growing season/pre-winter). Stations 1, 2, 3: 1.5mS, 0.5mW to NE corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. Refrigerated until sorted in the field lab (SECCE, SOXSU, TRFAL, HELAN, BRSNA (live volunteer canola (discard canola stubble left behind after harvest)), remainder label UNSRT. - Jon, Emily, Glori

LTER Post-harvest Extensive Grid soil sampling after corn harvest to send to A&L. Cores (0-25cm) taken in 5 East/West transects (avoiding sampling in the station flag walkways) using station flags as approximate guides for transects. 4 cores taken from each transect, 20 total cores per plot composited in 1 bag. T1-5 and T8NT sampled (waiting for harvest in T6 and lysimeter fetch). T5 was sampled using station flag locations in adjacent plots, not the condensed set-up present there. T8NT was 10 cores/plot sampled along a diagonal transect across each plot. - Jon, Emily, Glori, Jordyn

Sieved LTER Extensive Grid soil and removed subsamples to send to A&L - Jon, Emily, Glori, Jordyn

Started sorting ASP1 plants: MEDSA, TRFPR, CICSP, DACGL, BRSNA (live volunteer canola), remainder label UNSRT. - Jon, Emily, Glori

ASP1 forage sampling (end of growing season/pre-winter). Stations 1, 2, 3: 2mS, 0.5mE to NW corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. Refrigerated until sorted in the field lab. - Jon, Emily, Glori

Finish relocating soil archive from NE corner room for construction - Jon, Emily, Glori, Jordyn

Extract and filter LTAR R1-4 soil + chloroform for microbial CN - Stacey

Replace station flags in BCSE G4,5,7,9,10 - Jon, Emily

Collect leaf litter from T5, CF, DF, and SF - Glori, Jordyn

LTAR Series 27 gas sampling - Alyssa, Jon, Emily, Glori

LTAR Hydrosense - Emily

Finish sieving and subsampling GLBRC SVII soil. - Emily, Jon, Glori

Open LTAR soil chloroform tubes to vent in the hood. - Stacey

LTAR gas chamber prep - Emily, Glori, Jon

Sampled soil from Switchgrass Variety II (SVII) experiment, five 0-25cm cores pooled per split plot, 72 samples total - Emily, Glori, Jon

Weigh LTAR plot R3,4 0-10cm soil into 50mL centrifuge tubes for microbial CN. - Jon, Stacey

Sieve GLBRC SVII soil and subsample for A&L. - Jon, Glori, Emily

Extract and filter LTAR R3,4 unfumigated soil for microbial CN. Add chloroform to fumigated soils. Dishes - Stacey

LTAR gas vial prep - Alyssa, Stacey

Weigh LTAR plot R1,2 0-10cm soil into 50mL centrifuge tubes for microbial CN. Dishes - Jordyn, Stacey

Sieve LTAR R3,4 0-10cm soil from Sprunger lab. Sieve SBE soil and subsample for A&L. - Jon, Glori, Jordyn

Extract and filter LTAR R1,2 unfumigated soil for microbial CN - Stacey

Replace LTER T2-4 station flags - Jon, Glori

Sample GLBRC SBE soil, five 0-25 cm cores pooled per plot, sampled on a diagonal across the entire plot avoiding plot edges and ignoring the previous split plots - Jon, Glori, Jordyn

Dishes - Jon

Weigh LTAR and MLE Lux Arbor dry soil tins - Alyssa

Sieve LTAR R1,2 0-10cm soil from Sprunger lab. Continue to relocate soil archive samples. - Glori, Emily, Jon

Replace LTAR station flags with REACH - Glori, Emily

Temporarily relocate soil archive from the field lab to P.B. wash bay during field lab construction - Jon, Emily, Glori, Jordyn, Alyssa, Stacey

Finish fertilizing N-deposition microplots (SF2, R3 all) - Glori, Emily

Pull BCSE gas frames, flags for harvest - Stacey, Emily

Dishes - Jon

Filter MLE Lux Arbor soil extracts - Jon, Glori

Prepared 1M KCL.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepare and begin fertilizing N-deposition microplots (R1 all, DF2, CF2) - Jon, Glori

Weigh MLE Lux Arbor soil for extraction and moisture - Emily, Glori

Lux Arbor MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Pulled ground sensors on tower 4 BAU1F1 and found one heat flux plate chewed in pieces. Added sheathing to two sets of cables, dropped the sensor to 2m and added the temperature sensor for the EC100/Iragson. Reinstalled the sensors around 230PM.

Knocked over the remaining corn stalks around the tower, but did not collect the corn.

Collected SD cards on Towers 1-4, downloaded, saved to "ready for manager" and reformatted the cards.

Tower 2, releveled the IRGASON (it was pretty far off).

Dishes - Jon, Glori

Sieve MLE Lux Arbor soil - Emily, Glori, Jordyn

Acid washed dishes.

Ran water test for BAU1F1 flume yesterday. Abraham's homemade sampler worked well, it took most of the day however to determine pump issues with the ISCO. Resolved.

We used the large tank on wheels from Brook/Josh, filled, added an adapter from the tank to a garden hose and ran water through the hose to the front edge of the flume. We also added a block to the throat of the flume so the water level would increase faster.

We made need to change the size of the throat on all flumes to the smallest (0.75) flume size. Then adjust the code to calculate discharge.

FLUMES.

Hand biomass sampled MSG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

Replace station flags with Trimble in T1,2 - Emily, Jordyn, Jon

Help Chris and Todd sample MLE Lux soil - Emily, Glori

Finish sampling T4 corn ahead of harvest - Alyssa, Jon, Emily, Glori

LTAR Series 26 gas sampling - Jon, Emily, Glori, Jordyn

LTAR Hydrosense - Emily

Filter LTAR soil extracts, dishes, weigh dry MLE Lake City soil tins - Jon, Glori, Jordyn, Emily

Hand biomass sampled MCG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

Finish sieving LTAR plot soil - Alyssa, Emily

Weigh LTAR soil for extraction and moisture - Jon, Glori

Weigh dry MLE Escanaba soil tins - Stacey

LTAR gas vial, tote prep -

LTAR gas chamber prep - Emily, Alyssa

Collect leaf litter from traps on T5, CF, DF, SF - Alyssa, Emily, Glori, Jon

SGPK: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 2 cores per split. placed in a paper bag and put out to air dry

Sieve LTAR soil, remove subsamples for A&L S1 analysis from main plot soil only (not noNI microplots) - Alyssa, Emily, Jon, Glori

Filter MLE Lake City soil extracts - Jon, Glori

LTAR soil sampling (four 0-25cm cores/station, 12 cores/plot; noNI microplots 9 cores), took extra soil for A&L and archive - Alyssa, Emily, Jon, Glori

Finish sampling T3 corn ahead of harvest, start sampling T4 corn - Emily, Jon, Jordyn, Glori

Weigh MLE Lake City soil for extraction and moisture - Jordyn, Glori

Filter MLE Escanaba soil extracts - Emily, Jon

Prepared 1M KCL.

Finish sampling T1, T2 corn, start sampling T3 corn - Alyssa, Glori, Emily, Jon

Weigh MLE Escanaba soil for extraction and moisture -

Prepared 1M KCL

LTER Series 14 gas sampling - Jon, Glori, Jordyn, Emily, Stacey

LTER Hydrosense - Emily

BCSE Series 19 gas sampling - Alyssa, Glori

BCSE Hydrosense - Emily

LTER gas chamber prep - Jon, Emily

LTER T1R5 corn sampling - Alyssa, Glori, Jon, Emily

Sieve MLE MN Escanaba (sampled 10/13/2026) and MC Lake City (sampled 10/14/2026) soil samples - Alyssa, Glori, Emily, Jon

LTER field lab clean-up - Stacey

BCSE gas chamber prep - Glori

LTER T1, T2 corn sampling - Jon, Emily

Lake City MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

BCSE gas vial, tote prep - Glori

Filter MLE Rhinelander soil extracts - Glori, Alyssa

Escanaba MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Prepared 1M KCL.

Start sampling LTER corn from T1 and T2. Used 1.52 x 0.66 m frame; 3.04m S and 1.52m E from each station flag to NW frame corner, oriented with the 0.66m ends on the east and west. Perpendicular to corn rows. - Emily (am), Glori, Jordyn (pm)

Hand biomass sampled MNG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

Weigh MLE Rhinelander soil for extraction and moisture - Alyssa, Jordyn

Filter MLE Hancock soil extracts - Jon, Emily

Weigh MLE Hancock soil for extraction and moisture , dishes - Jon, Emily, Glori

Prepared 1M KCL.

Finish LTAR ASP2, BAU1 corn sampling - Alyssa, Jon, Emily, Glori

Finish MLE Rhinelander soil sieving - Alyssa, Olivia

Dishes - Alyssa

NAS data entry - Olivia

Reweigh (partially dry) and shred G2 sorghum samples, then weigh shredded - Alyssa, Olivia

Collect leaf litter from T5, CF, DF, and SF traps - Emily, Glori

Sample LTAR corn from ASP2, BAU1 - Emily, Glori

Mark NAS plots - Olivia

Sieve Hancock (10/6/2026) and Rhinelander (10/7/2026) soil. Sampled by Chris and Todd. - Emily, Glori, Alyssa

Weigh dry soil tins, dishes - Stacey

LTAR Series 25 gas sampling - Glori, Stacey, Emily, Alyssa

LTAR Hydrosense - Alyssa

LTAR gas chamber prep - Emily, Alyssa

Finish G6 ANPP sampling - Olivia, Glori, Emily, Alyssa

Start sampling LTAR corn, 0.66mS, 0.66mE to NW corner of 1.52 x 0.66m frame. Finished ASP2 R1 - Emily, Alyssa

Rhinelander MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Hand biomass sampled WNG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

LTAR gas vial, tote prep. Filter LTER soil extracts. Dishes - Olivia, Glori

Finish G6 plant species composition transects. Three NS transects per plot, main only. Each transect ~2m west of the station flags and numbered 1-3 based on the station number. Stop about every 1m to identify and measure first intercepted plant. G6 miscanthus (MISGI) ANPP plant sampling. Used 1.52 m x 0.66 m frame. All stations: 1.5mS, 1.5mE to NW corner of frame. - Alyssa, Emily, Jordyn, Glori, Olivia

Weigh LTER soil for extraction and moisture - Glori, Olivia

Hand biomass sampled WCG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with hedge clippers, and placed in burlap bag to be dried then weighed.

Hand biomass sampled MCSG11, One square meter, (0.5m X 2m), at 2 stations at locations in plot indicated on protocol map. Biomass was cut 6" above ground level with grass blade on brush cutter, and placed in burlap bag to be dried then weighed.

Hancock MLE: soil sampled all Nitrogen split plots with 3/4" push probe, 9" deep, 6 cores per split. placed in a plastic bag and put in cooler with ice until taken to KBS to be processed.

Prepared 1M KCL

Sieve LTER October soil. Remove 200g subsamples for Yang Lin from T1,2,4 R1,2,4,5. - Alyssa, Emily, Glori, Olivia

LTER October soil sampling. Plus, T1R1-3 and DF1-3 7cm depth, 20 cores/plot for Alison King. - Alyssa, Emily, Glori, Olivia

Collect LTAR leachate - Emily, Olivia

G6 plant species composition transects - Alyssa, Glori

Measure and filter LTAR leachate - Olivia, Emily

Pack LTAR 2024 plant CN - Jordyn, Olivia

Filter LTER T3, T4 soil extracts, dishes - Alyssa, Emily, Glori, Jordyn

Evacuate LTAR lysimeters - Emily, Alyssa

Weigh T3, T4 station and prairie strip soil for extraction and moisture - Olivia, Glori, Alyssa

Finish sorting ASP1 forage - Emily, Glori

Pack LTAR 2024 plant CN - Stacey, Alyssa

Prepared 1M KCL

Lowered tower 1 ASP3F1 back to 2m (6'7") and reinstalled ground sensors. Planted wheat this morning with blue donkey implement, leaves greater area around tower unplanted. Josh sowed seeds around tower this morning.

added IRGASON temp sensor to tower three at 9:30AM. ASP1F1 forage. After last cutting.

Sieve LTER T3, T4 station and prairie strip soil. Sort ASP1 forage - Alyssa, Olivia, Emily, Glori

Retrained Dawn GC gerstel sampler to injector, and tray. Agilent engineer changed entire front board, display and light now functioning.

Finished sampling G2 sorghum ANPP. Sampled T3, T4 station (2 cores/station) and prairie strip (4 cores/strip) soil. - Alyssa, Olivia, Emily, Glori

Acid washed dishes

BCSE G2 ANPP sorghum sampling, all stations: 1.5mS, 1.5mE to NW corner of 1.52x0.66m frame - Glori, Emily, Olivia (am), Jordyn, Alyssa (pm)

Raised tower 2 on BAU2F1 soybean around 1130 and removed ground sensors. Found two HFP cut and not really repairable.

Reinstalled ground sensors and lowered tower to 2m at 345PM.

Weigh G2 sorghum ANPP samples before putting into the oven - Glori, Emily, Jordyn, Olivia, Alyssa

Lab clean-up, update ChemInventory - Alyssa, Stacey

Weigh dry LTAR soil tins - Stacey

DawnGC run notes: LTAR Series 23, sampled 9/11/2026 - submitted from field with these notes - "vials 50 & 59 swapped, vial 129 broken." Samples all run on GC sequentially, and a dummy vial placed in the stead of vial 129.

Tower 1 over ASP3F1 raised to 12.5' at 11:30ish. Ground sensors removed also for soybean harvest. KK

Safety training - Alyssa

ChemInventory updating - Alyssa, Stacey

Dishes - Olivia

DawnGC run notes: NAS Series14, sampled 07/21/2026 - one set of four was submitted out of order as follows, Vial 17, 20, 19, 18. Vials all run sequentially on the GC, so these may need to be resorted, depending on field note references.

DawnGC run notes: BCSE Series 16 - vial 12 flipped upside down in sample tray. Guessing this means there was no sample taken, but vial flipped upright and analyzed to maintain continuity throughout the runs.

DawnGC run notes: LTER Series 12 - duplicate 3 sample vial was empty and vial 84 used a spare vial in its stead. All samples run sequentially on the GC with the spare vial replacing vial 84 and duplicate 3 kept in line as is.

Finished GLBRC Scale-up plant species composition transects - Alyssa, Olivia

BCSE G1 ANPP corn sampling, all stations: 1.5mS, 1.5mE to NW corner of 1.52x0.66m frame - Glori, Emily

Sort ASP1 forage - Olivia

LTER Series 13 gas sampling - Alyssa, Jon, Emily, Jordyn, Glori

LTER Hydrosense - Olivia

GLBRC Scale-up plant species composition transects - Jon, Alyssa

LTAR Series 24 gas sampling - Alyssa, Olivia, Jon, Glori

LTAR Hydrosense - Emily

Collect T5, CF, DF, and SF leaf litter from traps. Trim under T5 screens. - Alyssa, Glori, Olivia, Emily

Sort ASP1 forage - Olivia

Filter LTAR extracts, dishes - Jon, Glori

LTAR, LTER gas vial and tote prep - Stacey