Lachat run LTER T4 Soil 10/22/2024.

Prepared 1M KCL, Reconditioned Cadmium Column.

Lachat run LTER T3 soil 10/21/2024.

Lachat run LTAR soil 9/30/2024.

Acid washed dishes.

Lachat run GLBRC MLE WN soil (Rhinelander) 10/16/2024.

Prepared standards, prepared 1M KCL.

Lachat run CLE inorganic N (Hammond) June 2024 soil.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Lachat run LTAR soil 8/28/2024.

Reconditioned Cadmium Column.

Lachat run LTER Soil 9/17/2024.

Acid washed dishes.

Lachat run LTAR soil 7/23/2024

Prepared Standards, Acid washed dishes.

Lachat runs LTAR soil 5/28/2024 and 6/24/2024.

Lachat run LTER soil 8/14/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Reconditioned Cadmium Column, Acid washed all Channel 2 reagent containers.

Lachat run LTER soil T4 T21 7/8/2024.

Acid washed dishes, Lachat run LTER soil T3 T21 7/8/2024.

Prepared Standards, Lachat run LTER Soil 7/17/2024

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTAR Lysimeter 07/10/2024.

Vial ASP5 R1 S incorrectly labeled (listed as not enough liquid to be filtered). Ran as sample ASP5 R2 S as sample was missing from box. All vials from ASP1 R1 (N, C, S) missing from box, ran as blanks.

Acid washed dishes.

Lachat run LTER Lysimeter 06/05/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Lysimeter 05/21/2024.

Replaced Lachat reagent lines.

Replaced Cadmium column. Lachat run LTER Lysimeter 04/15/2024.

Lachat run LTER Lysimeter 04/01/2024

Prepared Standards, Reconditioned Cadmium Column.

Lachat run LTER Lysimeters 03/14/2024.

Acid washed dishes. Lachat run LTER T3 soil 7/08/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes, Lachat run LTER T4 soil 7/08/2024.

Reconditioned Cadmium Column. Lachat run LTER T-21 soil 7/01/2024.

Prepared standards, Lachat run LTER soil 6/19/2024.

Lachat runs GLBRC Soil 5/7/24, 5/30/24, 6/21/24 (not uploaded)

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Reconditioned Cadmium Column. Lachat runs LTAR soil 3/25/2024 and 4/29/2024.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Lachat run LTER T4 soil 6/3/2024

Prepared standards, Lachat run LTER T3 Soil 6/3/2024.

Lachat run LTER soil 6/5/2024.

Defrosted Shared freezer -20-2. Reconditioned Cadmium Column. Lachat run LTER soil 05/20/2024.

Acid washed dishes. Lachat run LTER Soil 5/8/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTER Soil 4/22/2024.

Prepared 1M KCL, standards. Acid washed dishes. Lachat run LTER soil 4/8/2024.

Lachat run LTER soil 3/12/2024

• Blanks registered as slightly more negative than usual. Made fresh KCL and standards to remove possible ammonia contamination. Blanks returned to normal range on next run.

Prepared standards

Finished dilutions from Lachat run LTAR Lysimeters 6/25/2024.

Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTAR lysimeters 6/25/2024

Lachat run LTER Lysimeters 11/14/2023 - finished with all 2023 samples.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Replaced Lachat reagent lines.

Lachat run LTER Lysimeter 10/18/2023.

Reconditioned Cadmium Column.

Lachat runs LTER Lysimeter 8/22/2023 and 9/26/2023.

Prepared standards, acid washed dishes.

Lachat run LTER Lysimeters 7/27/2023.

Lachat run LTER Lysimeters 7/13/2023

Lachat run LTER Lysimeters 6/28/2023.

Prepared Standards.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTER Lysimeters 6/13/2023

Prepared 1M KCL, Acid washed dishes.

Lachat run SWF soil 11/23/2024 (Ashim).

Prepared Standards, Replaced Cadmium Column.

Lachat run GLBRC Scale up Marshal Soil 12/7/2023 (Possible KCL contamination, not uploaded) -All 2023 soil sample Lachat runs completed

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run GLBRC Microplots 12/06/2023

Acid washed dishes

Prepared 1M KCL, prepared standards. Reconditioned Cadmium Column.

Lachat run GLBRC MLE MS (Lux Arbor) soi 11/29/2023 (G5R1-R4 shelter soil was sampled on 12/8/2023).

Lachat run LTER soil 11/06/2023

Lachat run GLBRC main soil 11/27/2023.

Prepared 1M KCL and Standards.

Lachat run GLBRC MLE MC soil (Lake City) 10/11/2023. Soil was Reextracted on 2/23/2024 due to contaminated KCL.

Acid washed dishes, Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run GLBRC Scale up Lux Arbor 12/07/2023.

Lachat run GLBRC MLE MN (Escanaba) soil 10/09/2023 - original sample had contaminated KCL, soil was reextracted on 2/21/2024.

Reconditioned Cadmium Column.

Acid washed dishes, Lachat run SWF 8/15/2024 soil (Ashim).

Prepared 1M KCL. Prepared Standards. Lachat run GLBRC MLE WN (Rhinlander) 10/04/2023 soil.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Replaced all reagent lines.

Prepared 1M KCL, Lachat run GLBRC MLE WN Rhinelander soil 10/4/2023. The dilutor malfunctioned during the run, only partially completed. The Dilutor was sent for repairs 8/6/2024, returned in September.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Acid washed dishes, Lachat run GLBRC MLE soil WC Hancock 10/3/2023.

Reconditioned Cadmium Column.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER T4 soil 09/18/2023

Prepared 1M KCL. Prepared standards. Lachat run LTER T3 soil 09/18/2023.

Lachat run LTER soil 08/08/2024. Acid washed dishes.

Lachat run LTER soil T4 (T-21) 7/17/23

Prepared standards, Lachat run LTER soil T3 (T-21) 7/17/24

Acid washed dishes, Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Replaced reagent lines, Reconditioned Cadmium column.

Lachat run SWF Lysimeters 2024 (Dave and Lydia).

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Acid washed dishes.

Finished dilutions from LTER T-21 soil Lachat run. Lachat run LTAR Lysimeters 6/10/2024.

Prepared Standards, prepared 1M KCL.

Lachat run LTER T-21 soil 7/5/2023.

Lachat run SWF soil 5/16/2024 and 6/3/2024 (Ashim and Elias).

Prepared 1M KCL.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Acid washed dishes.

Prepared Standards, Lachat run LTER soil 7/19/23.

Lachat run LTAR Lysimeter 5/28/2024.

Lachat run LTAR Lysimeter 5/9/2024. Reconditioned Cadmium column.

Lachat run LTER Lysimeter 5/11/2023

Acid washed dishes, prepared standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTER Lysimeter 04202023

Lachat run LTER Lysimeters 04/03/2023.

Prepared standards.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Acid washed dishes and reagent containers from Channel 1.

Lachat run LTER Lysimeters 03/21/23

Prepared Standards

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Acid washed dishes and Channel 2 Lachat reagent containers.

Lachat run LTER T4 soil 07/17/2023

Acid washed dishes. Lachat run LTER T3 soil 07/17/2023.

Replaced reagent tubing on Lachat.

Lachat run 9-25 and 9-28 2023 T0 post shelter REX (Grant).

Lachat run of dilutions from 8/28 and 8/30 2023 T0 Post drought REX (Grant).

Prepared standards, Lachat run 8/28 and 8/30 2023 T0 Post drought REX (Grant)

Prepared standards, reconditioned cadmium column, Acid washed dishes.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Replaced Cadmium Column, acid washed dishes.

Lachat run 8-14-2023 and 8-16-2023 T0 Peak drought REX soil (Grant).

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run 6-21-2023 and 6-23-2023 T0 Pre shelter REX soil (Grant)