Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Lachat run LTER Lysimeter 20250310.

-Vial SF1-3 had no ‘analysis’ sample, used ‘archive’ sample. Vial CF1-3 has volume listed as below 20 ml, run as missing vial CF2-3.

Prepared Standards, reconditioned cadmium column.

Lachat run LTAR lysimeter 20241209

• Samples BAU2 R2 N and ASP2 R3 C had vials filtered, but had volume listed as 15ml, ran samples as vials had enough sample for 3 samples and most likely had a volume at or above 20ml.
• Sample ASP2 R4 S had no volume listed, ran as missing vial ASP2 R4 C.

Finished all 2024 Lysimeter samples - LTAR files not uploaded.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTAR Lysimeter 20241125.

Replaced all reagent tubing on Lachat.

Lachat runs LTAR Lysimeter 20241031 and 20241114.

Lachat run LTAR Lysimeter 20240812. -Vial ASP1 R4 N has volume listed as not enough to filter, ran as missing vial BAU2 R4 S.

Reconditioned cadmium column.

Finished dilutions from Lachat run LTAR Lysimeter 20240725.

Prepared Standards, Acid washed dishes.

Lachat run LTAR Lysimeter 20240725 - Too many dilutions for today, will run next week. Sample labeled ‘ASP3 R3 N’ should not exist, vial ASP4 R3 N missing so ran as missing vial, labeled incorrectly?

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Lysimeters 20241107 and 20241216.

Reconditioned Cadmium Column.

Lachat run LTER Lysimeter 20240822 (Samples SF R2-3 and SF R2-6 may have been processed incorrectly. Vials from SF R2-3 have consistently had higher levels of NO3 than all other forest samples since installation, but a higher level was present in SF R2-6 for 8/22/2025.)

Lachat run LTER Lysimeter 20240801.

Prepared standards, prepared 1M KCL.

Acid washed dishes.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTAR Soil 20241120, 20241218.

All 2024 LTAR, LTER, and GLBRC soil samples processed - LTAR samples not uploaded.

Lachat run LTAR soil 20241028. Reconditioned Cadmium Column.

Finished Lachat run GLBRC Scale up Marshall 20241202, 20241216 - Blanks from 20241202 showed NH4 contamination in KCL (M1, M2, M4), not uploaded.

Lachat run GLBRC Scale up Marshall 20241202, 20241216 - half of run completed due to peek tubing malfunction. Replaced with new tubing, will finish rest of run tomorrow.

Prepared standards, prepared 1M KCL.

Lachat run GLBRC Scale up Lux Arbor 20241202, 20241216 - Blanks from 20241202 showed contamination in NH4 (L1 and L3 samples), not uploaded.

Reconditioned Cadmium Column.

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared 1M KCL.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared .1M HCL (1L) for column conditioning: 8.3 ml of 37% HCL in Nanopure, dilute to 1L.

Reconditioned Cadmium Column

Lachat run GLBRC MLE MC Soil (Lake City) 20241113.

Prepared Standards, Acid washed dishes.

Lachat run GLBRC MLE MN Soil (Escanaba) 20241112.

Lachat run LTER Lysimeter 20240715. -Vial labeled T4R3-2 does not have enough volume listed to filter, run as missing vial T4R2-3.

• Vial labeled T2R2-2 does not have enough volume listed to filter, run as missing vial T1R2-2.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Lysimeter 20240702.

Prepared Standards, Replaced Cadmium Column.

Lachat run LTER Lysimeters 20240617.

Reconditioned Cadmium Column. Lachat run LTER soil 20211112.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Replaced Reagent tubing, finished Lachat run GLBRC Microplot soil 20241111.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run GLBRC Microplot soil 20241111.

Prepared Standards, Lachat run GLBRC Main soil 20241104.

Acid washed dishes.

Recondition Cadmium Column. Lachat run GLBRC MLE MS soil (Lux Arbor) 20241106.

Prepared 1M KCL Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER soil 20241023.

Prepared Standards, Lachat run GLBRC MLE WC soil (Hancock) 20241017

Lachat run LTER T4 Soil 10/22/2024.

Prepared 1M KCL, Reconditioned Cadmium Column.

Lachat run LTER T3 soil 10/21/2024.

Lachat run LTAR soil 9/30/2024.

Acid washed dishes.

Lachat run GLBRC MLE WN soil (Rhinelander) 10/16/2024.

Prepared standards, prepared 1M KCL.

Lachat run CLE inorganic N (Hammond) June 2024 soil.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Lachat run LTAR soil 8/28/2024.

Reconditioned Cadmium Column.

Lachat run LTER Soil 9/17/2024.

Acid washed dishes.

Lachat run LTAR soil 7/23/2024

Prepared Standards, Acid washed dishes.

Lachat runs LTAR soil 5/28/2024 and 6/24/2024.

Lachat run LTER soil 8/14/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Reconditioned Cadmium Column, Acid washed all Channel 2 reagent containers.

Lachat run LTER soil T4 T21 7/8/2024.

Acid washed dishes, Lachat run LTER soil T3 T21 7/8/2024.

Prepared Standards, Lachat run LTER Soil 7/17/2024

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTAR Lysimeter 07/10/2024.

Vial ASP5 R1 S incorrectly labeled (listed as not enough liquid to be filtered). Ran as sample ASP5 R2 S as sample was missing from box. All vials from ASP1 R1 (N, C, S) missing from box, ran as blanks.

Acid washed dishes.

Lachat run LTER Lysimeter 06/05/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Lysimeter 05/21/2024.

Replaced Lachat reagent lines.

Replaced Cadmium column. Lachat run LTER Lysimeter 04/15/2024.

Lachat run LTER Lysimeter 04/01/2024

Prepared Standards, Reconditioned Cadmium Column.

Lachat run LTER Lysimeters 03/14/2024.

Acid washed dishes. Lachat run LTER T3 soil 7/08/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes, Lachat run LTER T4 soil 7/08/2024.

Reconditioned Cadmium Column. Lachat run LTER T-21 soil 7/01/2024.

Prepared standards, Lachat run LTER soil 6/19/2024.

Lachat runs GLBRC Soil 5/7/24, 5/30/24, 6/21/24 (not uploaded)

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Reconditioned Cadmium Column. Lachat runs LTAR soil 3/25/2024 and 4/29/2024.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Lachat run LTER T4 soil 6/3/2024

Prepared standards, Lachat run LTER T3 Soil 6/3/2024.

Lachat run LTER soil 6/5/2024.

Defrosted Shared freezer -20-2. Reconditioned Cadmium Column. Lachat run LTER soil 05/20/2024.

Acid washed dishes. Lachat run LTER Soil 5/8/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTER Soil 4/22/2024.

Prepared 1M KCL, standards. Acid washed dishes. Lachat run LTER soil 4/8/2024.

Lachat run LTER soil 3/12/2024

• Blanks registered as slightly more negative than usual. Made fresh KCL and standards to remove possible ammonia contamination. Blanks returned to normal range on next run.

Prepared standards

Finished dilutions from Lachat run LTAR Lysimeters 6/25/2024.

Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTAR lysimeters 6/25/2024

• Vials labeled BAU1 R3 N,C,S were listed as having volumes of 10,10, and 0 ml, run as missing vials ASP3 R1 N,C,S.

Lachat run LTER Lysimeters 11/14/2023 - finished with all 2023 samples.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Replaced Lachat reagent lines.

Lachat run LTER Lysimeter 10/18/2023.

Reconditioned Cadmium Column.

Lachat runs LTER Lysimeter 8/22/2023 and 9/26/2023.

Prepared standards, acid washed dishes.

Lachat run LTER Lysimeters 7/27/2023.

Lachat run LTER Lysimeters 7/13/2023

Lachat run LTER Lysimeters 6/28/2023.

Prepared Standards.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTER Lysimeters 6/13/2023

Prepared 1M KCL, Acid washed dishes.

Lachat run SWF soil 11/23/2024 (Ashim).

Prepared Standards, Replaced Cadmium Column.

Lachat run GLBRC Scale up Marshal Soil 12/7/2023 (Possible KCL contamination, not uploaded) -All 2023 soil sample Lachat runs completed

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run GLBRC Microplots 12/06/2023

Acid washed dishes

Prepared 1M KCL, prepared standards. Reconditioned Cadmium Column.

Lachat run GLBRC MLE MS (Lux Arbor) soi 11/29/2023 (G5R1-R4 shelter soil was sampled on 12/8/2023).

Lachat run LTER soil 11/06/2023

Lachat run GLBRC main soil 11/27/2023.

Prepared 1M KCL and Standards.

Lachat run GLBRC MLE MC soil (Lake City) 10/11/2023. Soil was Reextracted on 2/23/2024 due to contaminated KCL.

Acid washed dishes, Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run GLBRC Scale up Lux Arbor 12/07/2023.

Lachat run GLBRC MLE MN (Escanaba) soil 10/09/2023 - original sample had contaminated KCL, soil was reextracted on 2/21/2024.

Reconditioned Cadmium Column.

Acid washed dishes, Lachat run SWF 8/15/2024 soil (Ashim).

Prepared 1M KCL. Prepared Standards. Lachat run GLBRC MLE WN (Rhinlander) 10/04/2023 soil.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Replaced all reagent lines.

Prepared 1M KCL, Lachat run GLBRC MLE WN Rhinelander soil 10/4/2023. The dilutor malfunctioned during the run, only partially completed. The Dilutor was sent for repairs 8/6/2024, returned in September.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Acid washed dishes, Lachat run GLBRC MLE soil WC Hancock 10/3/2023.

Reconditioned Cadmium Column.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER T4 soil 09/18/2023

Prepared 1M KCL. Prepared standards. Lachat run LTER T3 soil 09/18/2023.

Lachat run LTER soil 08/08/2024. Acid washed dishes.