Projectlog Entries List

Lachat run LTER Lysimeter 20260402. Notes from run:

• Three vials labeled T3 R3 1, 2, and 3 were ran as T5 R3 1, 2, and 3. Flasks for T3 R3 and T5 R3 were swapped when evacuating samples. Weights were adjusted when entering in data file to the correct location, but vials were sampled incorrectly.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER Lysimeter 20260319. Notes from run:

• Vial labeled T2 R4 1 should not exist, not enough volume listed. Ran as Vial T3 R4 1, that was not present and should exist.
• Vial T CF R2 1 missing from box, not run (no data notes)
• Vial T6 R3 3 present, should not exist. Not enough volume in vial to run.

Prepared Standards.

Lachat run LTAR Lysimeter 20260616. Notes from run:

• Vial labeled ASP2 R1 N should not exist, not enough volume. Ran as vial ASP2 R2 N, that should exist and is missing.
• Vial ASP6 R2 S was missing from box and should exist, not run.

Prepared Standards. Lachat run LTAR Lysimeter 20260602.

Reconditioned Cadmium Column.

Finished dilutions from LTAR Lysimeter 20260519 run.

Prepared 1M KCL.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTAR Lysimeter 20260519. Notes from run:

• Samples ASP1 R4 C, S, and ASP3 R3 C were not present, not analyzed.

Acid washed dishes. Lachat run LTAR Lysimeter 20260505. Notes from run:

• Samples ASP3 R1 N and C were dumped before filtering, not run.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTAR Lysimeter 20260421.

Prepared standards. Lachat run LTAR Lysimeter 20260407. Notes from run:

• Vial ASP1 R1 S missing from box, not run.

Acid washed dishes.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared 1M KCL. Replaced all Lachat reagent tubing.

Reconditioned Cadmium Column.

Lachat run LTAR Lysimeter 20260326. Notes from run:

• Sample BAU2 R2 N ran as sample BAU2 R4 N. BAU2 R4 N should have sample present, and BAU2 R2 N had volume listed as 0 ml
• Sample labeled as ASP1 R1 S was present, but volume was listed as 5ml (vial was full). No other missing samples, so ran as labeled. Volume listed incorrect?

Acid washed dishes

Finished dilutions for Lachat run LTAR Lysimeter 20260309.

Lachat run LTAR Lysimeter 20260309 notes from run:

• Vial ASP1 R1 N present, not enough volume listed to filter. Ran as vial ASP1 R1 S that should exist and was not present in sample box.
• Vials ASP2, R3 N, ASP4 R1 N, AND BAU2 R2 S all listed as not enough volume to filter. No other missing vials, volumes listed incorrect? Vial BAU2 R2 S not run, only 1/4th full, not enough to run sample.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Lysimeter 20251119

All 2025 soil and Leachate LTER, LTAR, and GLBRC Lachat samples complete

Prepared Standards

Lachat run LTER Lysimeter 20251027

Acid washed dishes.

Finished dilutions for Lachat run LTER Lysimeter 20250929.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Reconditioned Cadmium Column.

Lachat runs LTER Lysimeters 20250908, 20250929.

Prepared Standards, Lachat run LTER Lysimeter 20250814

Prepared Standards.

Lachat run LTER Lechate 20250721. Notes from run:

• Sample vials T7 R3 1, 2, and 3 were missing from set. Vials labeled T6 R3 1 and 2 do not have enough volume listed to be filtered and should not exist. Ran Vials T6 R3 1 as T7 R3 1 and T6 R3 2 as T7 R3 2. T7 R3 3 not run.

Acid washed dishes (acid washed all channel 1 reagent containers).

Reconditioned Cadmium Column.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run SWF 2024 Lysimeters Set 4 dilutions.

Prepared Standards.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run SWF 2024 Lysimeters Set 4 (20456-20498)

• Samples 20499-20514 were not run, unable to locate samples.

Acid washed dishes

Lachat run 2024 SWF Lysimeters Set 3 (20401-20455).

Acid washed dishes.

Lachat run 2024 SWF Lysimeters Set 2 (20330-20401).

Prepared Standards.

Lachat run 2024 SWF Lysimeter Set 1 (20279-20329)

Prepared Standards. Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Lachat run LTER Lysimeter 20250630. Notes from run:

• Sampled labeled DF1-6 was listed as having a volume too small to filter sample. No vials were missing from set, so volume listed may be incorrect?

Acid washed dishes, Acid washed channel 2 reagent containers. Replaced Lachat reagent tubing.

Reconditioned Cadmium Column. Lachat run LTER Lysimeter 20250617. Notes from run:

• Sample SF2-1 not run, not enough liquid in vial
• Sample T7 R3-3 Missing from box, not run

Prepared 1M KCL. Lachat run LTER Lysimeter 20250603. Notes from run:

• Volumes of flasks when weighing were not recorded correctly, tare was incorrect. Weights listed are now corrected, but this impacted samples filtered.
• Flasks T5R4-1 and T7R3-3 should have been filtered, but volume at time of weighing was listed as less than 20ml. Not run.
• Flasks T6R3-3 and T6R4-3 should not have been filtered, but volume at time of weighing was listed as more than 20ml. Vials were run and results included.

Lachat run MDARD Climate Resiliency 2025 Soil Set 2.

Lachat runs MDARD Climate Resiliency soil 2025 (dilutions) and Warm x 2025 Soil Nitrogen (Zarnetske lab).

Prepared Standards. Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O. Reconditioned Cadmium Column.

Lachat run MDARD Climate Resiliency soil 2025 Set 1. Dilutions to be finished tomorrow.

Prepared standards Lachat run LTER Lysimeter 20250520. Notes from run:

• Samples T6R4-2, T7R2-3, and T7R4-1 were missing from box. Two vials labeled T7R2-2 and T7R4-3 shouldn't exist. Unable to determine which sample the two vials were mislabeled as and results not used.

Prepared 1M KCL, standards.

Lachat run LTER Lysimeter 20250506. -Sample DF3-1 was missing from set. Vial DF3-3 should not exist, ran as sample DF3-1.

Acid washed dishes.

Lachat run LTER Lysimeter 20250421. Run notes:

• Samples T1 R2-2 and T7 R2-1 should have samples, but no vial was in sampled box, skipped.
• Sample CF1-1 has no vial, should exist. Vial labeled as CF1-6 does not have enough volume listed to filter, ran as CF1-1.

Prepared 1M KCL, Lachat run LTER Lysimeter 20250408.

Replaced end filter and 4th cartridge filter on Barnstead nanopure. Ran water test on Lachat, filter seems to be functioning normally again.

Uploaded GLBRC MLE MC (Lake City) 20251125 soil samples. We used the re-extracted G11 soil samples in place of the original samples due to possible incorrectly labeled vials.

All 2025 LTER, LTAR, and GLBRC Lachat soil run uploaded.

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Finished GLBRC Microplot soil 20251203 Lachat run and Re-extracted GLBRC MLE MC (Lake City) soil 20251125.

Lachat run GLBRC Microplot Soil 20251203.

• unable to finish end of run, will continue on 3/26/26 (not uploaded).

Lachat run GLBRC soil Main plots 20251201.

Prepared standards, Lachat run LTAR soil 20251208.

• No vial ASP5 R2 a, missing from box. Ran as blank.

Lachat run GLBRC MLE WC soil 20251119 (Hancock).

Lachat run GLBRC MLE MC soil 20251125 (Lake City). Notes from run: -No G8R3 N c sample, spilled when filtering, ran as blank.

• G6R3 N b and G6R3 S b samples may have swapped - ran in vial order.
• Many G11 samples may have been labeled incorrectly. G11 Samples to be reextracted to cross check on correct grouping and vial order. Not uploaded for now.

Lachat run GLBRC Soil MLE WN 2025118 (Rhinelander)

• G6R2 E a and G6R2 W a vials may have been swapped, ran in vial box order.

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Standards, Lachat run GLBRC MLE MN soil 20250123 (Escanaba)

Lachat run GLBRC MLE MS soil 20251118 (Lux Arbor).

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER 20251117.

Lachat run LTER soil 20251015.

Acid washed dishes, Replaced Cadmium column.

Lachat run LTER soil 20250820 (Re-extracted on 2/24/2026 due to KCL contamination)

• 1 bin of soil bags from set was in a malfunctioning walk-in cooler (90 degrees). Noted below are those bags: T4 R1-R3, R5, T5 R1-R6, T6 R1-R6, T7 R1-R6

Prepared 1m KCL

Lachat run LTER soil 20250806 (Re-extracted on 2/23/26 to compare to contaminated samples)

Lachat run LTER T4 soil 20250723 (Re-extracted on 2/18/26 due to KCL contamination)

Prepared 1M KCL

Lachat run LTER T3 soil 20250723 (Re-extracted on 2/18/2026 due to KCl contamination)

Acid washed dishes, reconditioned cadmium column.

Lachat run LTAR 20251103.

Prepared Standards, Replaced all reagent tubing.

Lachat run LTAR 20251008.

Lachat run LTER T4 soil 20250929.

Prepared 1M KCL.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER T3 Soil 20250929.

Acid washed dishes. Lachat run LTER Soil 20250915.

Lachat run LTER Soil 20250820 - KCL Contamination in Channel 1 blanks (NH4 results). Not uploaded for now.

Lachat run LTER soil 20250806.

Prepared Standards, Lachat run LTAR soil 20250908.

Acid washed dishes, reconditioned cadmium column.

Lachat run LTAR soil 20250813.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Lachat run LTER T4 T21 Soil 20250723.

• Samples T4R2S4, T4R4S1, and T4R4 strip were not processed, not included.

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER T21 Soil 20250714

• No CF1 samples were processed, not included.

Acid washed dishes, prepared Standards.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER T4 soil 20250723. Contamination in KCL blanks in NH4 results, not uploaded.

Lachat run LTER T3 Soil 20250723. KCL NH4 contamination in blanks. Not uploaded.

Lachat run LTER soil 20250714.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Soil 20250616.

Reconditioned Cadmium Column, Acid washed dishes.

Lachat run LTER T4 soil 20250527

Replacement dilutor test against hand dilutions prepared last week to test for accuracy - consistent.

Prepared Standards. Lachat run LTER Soil 20250602.

Lachat runs GLBRC soil 20250623, LTAR soil 20250709 (diluted by hand).

Reconditioned Cadmium Column

Prepared standards (hand diluted)

Lachat run GLBRC soil 20250522 (re-ran part of run from 12/22/2025 to compare samples and hand dilution standards to dilutor standards- consistent)

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run GLBRC 20250430, part of 20250522 (dilutor malfunction - refroze all samples to run at a later date)

Acid washed all Channel 1 reagent containers. Replaced all reagent tubing.

Acid washed dishes, reconditioned Cadmium column.

Lachat run LTER T3 soil 20250527.

Lachat run LTAR soil 20250609. Notes from run (also noted when uploaded):

• Vials ASP3 R4 B and ASP3 R5 B swapped in order in box. Ran as labeled but may have been labeled incorrectly due to results.
• 3 sets of Vials may have been labeled incorrectly due to results, ran as labeled:

ASP3 R2 A and ASP3 R3 A

BAU1 R4 C and BAU2 R4 C

BAU1 R5 C and BAU2 R5 C

(discounted vials when uploading due to large CV)

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Acid washed dishes.

Lachat run MDARD Soil September.

Prepared Standards, Acid washed dishes and all Channel 2 reagent containers.

Lachat run LTAR Lysimeter 20251210.

All 2025 LTAR Lysimeter Lachat samples run and uploaded

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Finished dilutions from Lachat run LTAR Lysimeter 20251124.

Reconditioned Cadmium Column

Acid washed dishes.

Prepared standards, Lachat run LTAR Lysimeter 20251124.

Finished Dilutions from Lachat run LTAR Lysimeter 20251112.

Prepared 1M KCL. Finished dilutions from Lachat run LTAR Lysimeter 20251021.

Lachat run LTAR Lysimeter 20251112. Notes from run:

-Vial BAU1 R4 C most likely mislabeled as BAU2 R4 C (vial shouldn't exist)

-Vial BAU2 R2 S most likely mislabeled as BAU2 R1 S (vial shouldn't exist)

Lachat runs LTAR Lysimeter 20251001, 20251021. Notes from runs:

-20251001 - Vial BAU2 R1 S was missing from box, not run. Unusually high NH4 results for bottle ASP1 R3 N, contaminated?

-20251021 - Vial ASP3 R4 S was missing from box, not run. Vial BAU2 R4 N potentially mislabeled as BAU2 R4 C, or volume may have been listed incorrectly on data sheet. Bottle C is listed as having pressure, but no liquid recorded, where N had no pressure. Ran as vial BAU2 R4 N. No blanks were included, used fresh RO water.

Prepared 1M KCL, standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared 1M KCL.

Lachat runs LTAR Lysimeter 20250728, 20250821, and 20250915.

Acid wished dishes. Lachat run LTAR Lysimeter 20250714: BAU1 R4 C bottle was spilled, no samples were filtered/run.

Prepared Standards, Lachat run LTAR Lysimeter 20250624.

Prepared 1M KCL. Finished dilutions from LTAR Lysimeter 20250610.

Lachat run LTAR Lysimeter 20250610.

Prepared 1M KCL, Lachat run LTAR Lysimeter 20250529.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Reran Channel 2 and dilutions of Lachat run LTAR Lysimeter 20250513.

Replaced Cadmium Column and reconditioned.

Lachat run LTAR Lysimeter 20250513. Notes from run:

• Vial ASP2 R3 C not run, vial missing from box.

Issue with calibration curve/column in channel 2 results - rerun 11/14.

Acid washed dishes, Prepared standards.

Lachat run LTAR Lysimeter 20250428. Notes from run:

• Vial ASP5 R2 N missing, used vial ASP5 R1 N that shouldn't exist (mislabeled?)
• Vial BAU1 R1 C not run, missing from box.
• Vial BAU2 R3 N has no volume listed, mislabeled or volume missed? Ran as labeled.