Sodium salicylate is discolored and when in solution with nanopure turns dark brown. Not suitable for assay. Called VWR and also spoke with a rep from Alfa Aesar. Ordered 2 replacement bottles-Cathy Acid wash dishes, cleaning bottles for shipment with Alpkem-Cathy

Two Lachat runs: first was 2014 LTER lysimeters, 20140402 dilutions at end of run on 3/18/15. (did not have time to finish run on Wednesday). OM_3-23-2015_08-40-46AM.OMN. Second run: 2014 LTER lysimeters 20140430, OM_3-23-2015_09-47-06AM.OMN. Regenerated column prior to running.-Cathy Prepared Color Reagent (1 L) 100 ml phosphoric acid, Lot #71420 40 gm sulfanilamide, Lot #MKBR8668V 1.0 gm N-1-naphthylethylenediamine dihydrochloride, Lot #MKBR4581V Dilute to 1L with Nanopure H2O, store. -Cathy Acid wash dishes.-Cathy Took pics of Alpkem and made list of components with model and serial numbers. Stored \\LTERNT5\data entry\Winflow_Alpkem\Pics -Cathy

Acid wash dishes, review standard data with Kevin, ORCBS-Cathy

Alpkem run, lach.rst. Standard comparisons with Lachat. Recharged coil prior to run. Cathy Prepared Ammonium Chloride Reagent (1L): 80 gms Ammonium Chloride (Ampresco) Lot # 1324c135 0.1 gms EDTA Lot # 10186054 Approximately 7 mL NaOH, pH to 8.48-Cathy Sodium Nitroferricyanide: 0.5g lot # H47599. Nanopure water to 1L, filter. -Cathy Prepared Citrate reagent (1L): 31.5 grams sodium citrate #57457 JT Baker 1 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 1 liter, filter-Cathy Acid wash dishes-Cathy

Found \”missing\” samples LTER 6/16/2014. Had been analyzed already on 7/2/14 but had been exported as 20130616. Changed sample names in text file and also file name. Will not upload, Sven notified. Also OM11-12-2014-01-34-55PM.OMN will not upload.-Cathy

Alpkem run, 20140722.rst, LTER 2014 soil. Recharged coil at end of run.-Cathy Prepared Citrate reagent (1L): 31.5 grams sodium citrate #57457 JT Baker 1 gram sodium hydroxide pellets, Macron Brand, lot #

52566. Nanopure water to 1 liter, filter-Cathy Acid wash dishes-Cathy

Prepared Ammonium Chloride Reagent (2L): 170 gms Ammonium Chloride, BDH brand 0.2 gms EDTA Lot # 083759A Approximately 15mL Sodium Hydroxide Lot # K31507, pH to 8.54, filter-Cathy Acid wash dishes-Cathy

Alpkem run, 2013 samples for Victoria Akroyd (VA2013.rst). Emailed results to Victoria.-Cathy

Alpkem run, T21 soil LTER 2014, 20140714.rst-Cathy

Alpkem run, LTER 2014 soil, 20140709.rst. Acid wash dishes-Cathy

Prepare table for tomorrow\’s run, acid wash dishes-Cathy

Cleaned Alpkem, 1 M HCl through channel #1, 1 M NaOH through channel #2. Acid wash dishes. Prepared fresh 1 M HCl for cleaning. Organize data to compare Alpkem runs and Lachat runs.-Cathy

Alpkem run, 20140512.rst, LTER 2014 soil. Prepared fresh standards except CCV and NO2 eff. which were prepared yesterday for Lachat run. Recharged coil yesterday (#12037D)-Cathy

Cleaned NH4 channel flow cell-Cathy

Alpkem run, 2nd 1/2 of samples from yesterday, LTER soil 2014 0414. (2014414b.rst). Paused run after first 12 samples as orange crud was building up in NH4 channel. Cleaned channel, restarted, looked good. Acid wash dishes-Cathy

Alpkem run, 20140414.rst, LTER 2014 soil. Only had time for 1/2 run, will run remaining samples tomorrow.-Cathy Acid wash dishes-Cathy Prepare fresh Sodium Nitroferricyanide-Cathy

Alpkem run, 20140414 LTER soil samples-Cathy

Alpkem run, Sarah Roley\’s samples that were affected by bad NH4 baseline last week (orange crud build up) Lachat recommends Fisher sodium hypochlorite to prevent discoloration (ordered today). Suspect the grocery store bleach used recently is causing extra buildup. Emailed Sarah\’s results. Cleaned NH4 channel with 1 N HCl. Acid wash dishes.-Cathy

Alpkem run, 2014 LTER soil, 20140429. Same samples run on Lachat yesterday to compare results. Baseline was rising on NH4 channel and debubbler looked a little dirty. Also NO3 baseline was not great, all low results dipping way below baseline. Will rerun all tomorrow, not reporting anything from this run. Cleaned NH4 channel with HCl. -Cathy Prepared Ammonium Chloride Reagent (4L): 340 gms Ammonium Chloride, BDH brand 0.4 gms EDTA Lot # 083759A Approximately 30mL Sodium Hydroxide Lot # K31507, pH to 8.48, filter-Cathy Prepared Citrate reagent (2L): 63 grams sodium citrate #57457 JT Baker 2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 2 liters, filter-Cathy Acid wash dishes-Cathy

Ran LTER 2014 soil samples, 20140429, on Lachat. First full run on new instrument, went well. Will run samples on Alpkem tomorrow to compare.-Cathy Upload GLBRC incubation data (2013)-Cathy Spoke to \”Pat\” at ORCBS. He said there is no need to change our waste code for Ammonium waste. Also the new chemicals that will be added to the waste will be no problem. He also said we can combine the nitrate and ammonium waste in one container if we prefer to.-Cathy Prepared Color Reagent (1 L) 100 ml phosphoric acid, Lot #60732 40 gm sulfanilamide, Lot #SLBD9423V 1.0 gm N-1-naphthylethylenediamine dihydrochloride, Lot #12120MC (borrowed from Lichtman lab) Dilute to 1L with Nanopure H2O, store. -Cathy Acid wash dishes-Cathy

Acid wash dishes-Cathy

Set up Alpkem for temporary use with new Lachet. Upload LTER 2013 N-data. Review new Lachet methods and order chemicals that we do not have in stock. Talked to Lachet tech support regarding new methods vs Alpkem methods.-Cathy

Upload LTER N data-Cathy

Alpkem run, LTER soil 20131112.rst. Ran out of alkaline phenol during run. Prepared new and restarted but first two CCVs were too low—suspect I didn\’t let the new alkaline phenol run through for long enough since it corrected itself by the end of the run. Will reinject affected samples again (run for NH4 only, running brij/H2O through NO3 channel-Cathy

Alpkem run, LTER 2013 soil, 20131014.rst. Peak shape for NO3 still not great, suspect coil is worn out. Using new coil, 12037X, charged and first used today. Peak shape excellent. Cathy Prepared fresh Nitroferricyanide Reagent: Sodium Nitroferricyanide: 0.5g lot # H47599. Nanopure water to 1L, filter. -Cathy Prepared Ammonium Chloride Reagent (4L): 340 gms Ammonium Chloride NEW LOT# 156297, BDH brand 0.4 gms EDTA Lot # 083759A Approximately 30mL Sodium Hydroxide Lot # K31507, pH to 8.55, filter-Cathy Acid wash dishes-Cathy