Sodium salicylate is discolored and when in solution with nanopure turns dark brown. Not suitable for assay. Called VWR and also spoke with a rep from Alfa Aesar. Ordered 2 replacement bottles-Cathy Acid wash dishes, cleaning bottles for shipment with Alpkem-Cathy

Two Lachat runs: first was 2014 LTER lysimeters, 20140402 dilutions at end of run on 3/18/15. (did not have time to finish run on Wednesday). OM_3-23-2015_08-40-46AM.OMN. Second run: 2014 LTER lysimeters 20140430, OM_3-23-2015_09-47-06AM.OMN. Regenerated column prior to running.-Cathy Prepared Color Reagent (1 L) 100 ml phosphoric acid, Lot #71420 40 gm sulfanilamide, Lot #MKBR8668V 1.0 gm N-1-naphthylethylenediamine dihydrochloride, Lot #MKBR4581V Dilute to 1L with Nanopure H2O, store. -Cathy Acid wash dishes.-Cathy Took pics of Alpkem and made list of components with model and serial numbers. Stored \\LTERNT5\data entry\Winflow_Alpkem\Pics -Cathy

Acid wash dishes, review standard data with Kevin, ORCBS-Cathy

Alpkem run, lach.rst. Standard comparisons with Lachat. Recharged coil prior to run. Cathy Prepared Ammonium Chloride Reagent (1L): 80 gms Ammonium Chloride (Ampresco) Lot # 1324c135 0.1 gms EDTA Lot # 10186054 Approximately 7 mL NaOH, pH to 8.48-Cathy Sodium Nitroferricyanide: 0.5g lot # H47599. Nanopure water to 1L, filter. -Cathy Prepared Citrate reagent (1L): 31.5 grams sodium citrate #57457 JT Baker 1 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 1 liter, filter-Cathy Acid wash dishes-Cathy

Found \”missing\” samples LTER 6/16/2014. Had been analyzed already on 7/2/14 but had been exported as 20130616. Changed sample names in text file and also file name. Will not upload, Sven notified. Also OM11-12-2014-01-34-55PM.OMN will not upload.-Cathy

Alpkem run, 20140722.rst, LTER 2014 soil. Recharged coil at end of run.-Cathy Prepared Citrate reagent (1L): 31.5 grams sodium citrate #57457 JT Baker 1 gram sodium hydroxide pellets, Macron Brand, lot #

52566. Nanopure water to 1 liter, filter-Cathy Acid wash dishes-Cathy

Prepared Ammonium Chloride Reagent (2L): 170 gms Ammonium Chloride, BDH brand 0.2 gms EDTA Lot # 083759A Approximately 15mL Sodium Hydroxide Lot # K31507, pH to 8.54, filter-Cathy Acid wash dishes-Cathy

Alpkem run, 2013 samples for Victoria Akroyd (VA2013.rst). Emailed results to Victoria.-Cathy

Alpkem run, T21 soil LTER 2014, 20140714.rst-Cathy

Alpkem run, LTER 2014 soil, 20140709.rst. Acid wash dishes-Cathy

Prepare table for tomorrow\’s run, acid wash dishes-Cathy

Cleaned Alpkem, 1 M HCl through channel #1, 1 M NaOH through channel #2. Acid wash dishes. Prepared fresh 1 M HCl for cleaning. Organize data to compare Alpkem runs and Lachat runs.-Cathy

Alpkem run, 20140512.rst, LTER 2014 soil. Prepared fresh standards except CCV and NO2 eff. which were prepared yesterday for Lachat run. Recharged coil yesterday (#12037D)-Cathy

Cleaned NH4 channel flow cell-Cathy

Alpkem run, 2nd 1/2 of samples from yesterday, LTER soil 2014 0414. (2014414b.rst). Paused run after first 12 samples as orange crud was building up in NH4 channel. Cleaned channel, restarted, looked good. Acid wash dishes-Cathy

Alpkem run, 20140414.rst, LTER 2014 soil. Only had time for 1/2 run, will run remaining samples tomorrow.-Cathy Acid wash dishes-Cathy Prepare fresh Sodium Nitroferricyanide-Cathy

Alpkem run, 20140414 LTER soil samples-Cathy

Alpkem run, Sarah Roley\’s samples that were affected by bad NH4 baseline last week (orange crud build up) Lachat recommends Fisher sodium hypochlorite to prevent discoloration (ordered today). Suspect the grocery store bleach used recently is causing extra buildup. Emailed Sarah\’s results. Cleaned NH4 channel with 1 N HCl. Acid wash dishes.-Cathy

Alpkem run, 2014 LTER soil, 20140429. Same samples run on Lachat yesterday to compare results. Baseline was rising on NH4 channel and debubbler looked a little dirty. Also NO3 baseline was not great, all low results dipping way below baseline. Will rerun all tomorrow, not reporting anything from this run. Cleaned NH4 channel with HCl. -Cathy Prepared Ammonium Chloride Reagent (4L): 340 gms Ammonium Chloride, BDH brand 0.4 gms EDTA Lot # 083759A Approximately 30mL Sodium Hydroxide Lot # K31507, pH to 8.48, filter-Cathy Prepared Citrate reagent (2L): 63 grams sodium citrate #57457 JT Baker 2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 2 liters, filter-Cathy Acid wash dishes-Cathy

Ran LTER 2014 soil samples, 20140429, on Lachat. First full run on new instrument, went well. Will run samples on Alpkem tomorrow to compare.-Cathy Upload GLBRC incubation data (2013)-Cathy Spoke to \”Pat\” at ORCBS. He said there is no need to change our waste code for Ammonium waste. Also the new chemicals that will be added to the waste will be no problem. He also said we can combine the nitrate and ammonium waste in one container if we prefer to.-Cathy Prepared Color Reagent (1 L) 100 ml phosphoric acid, Lot #60732 40 gm sulfanilamide, Lot #SLBD9423V 1.0 gm N-1-naphthylethylenediamine dihydrochloride, Lot #12120MC (borrowed from Lichtman lab) Dilute to 1L with Nanopure H2O, store. -Cathy Acid wash dishes-Cathy

Acid wash dishes-Cathy

Set up Alpkem for temporary use with new Lachet. Upload LTER 2013 N-data. Review new Lachet methods and order chemicals that we do not have in stock. Talked to Lachet tech support regarding new methods vs Alpkem methods.-Cathy

Upload LTER N data-Cathy

Alpkem run, LTER soil 20131112.rst. Ran out of alkaline phenol during run. Prepared new and restarted but first two CCVs were too low—suspect I didn\’t let the new alkaline phenol run through for long enough since it corrected itself by the end of the run. Will reinject affected samples again (run for NH4 only, running brij/H2O through NO3 channel-Cathy

Alpkem run, LTER 2013 soil, 20131014.rst. Peak shape for NO3 still not great, suspect coil is worn out. Using new coil, 12037X, charged and first used today. Peak shape excellent. Cathy Prepared fresh Nitroferricyanide Reagent: Sodium Nitroferricyanide: 0.5g lot # H47599. Nanopure water to 1L, filter. -Cathy Prepared Ammonium Chloride Reagent (4L): 340 gms Ammonium Chloride NEW LOT# 156297, BDH brand 0.4 gms EDTA Lot # 083759A Approximately 30mL Sodium Hydroxide Lot # K31507, pH to 8.55, filter-Cathy Acid wash dishes-Cathy

Cleaned both channels; NO3 with NaOH and NH4 with HCl. HCl was left in lines overnight to clean. Acid wash dishes-Cathy

Alpkem run, LTER 2013 soil, 20130916.rst. Kevin\’s samples added to this run also. Acid wash dishes-Cathy

Filter KCl, prepare fresh standards-Cathy

Prepared Ammonium Chloride Reagent (2L): 170 gms Ammonium Chloride NEW LOT# 156297, BDH brand 0.2 gms EDTA Lot # 083759A Approximately 20mL Sodium Hydroxide Lot # K31507, pH to 8.55, filter-Cathy Alpkem run, second part of yesterday\’s run, 2013722B.rst-Cathy

Alpkem run, 20130722.rst. LTER soil. Had to stop run after NO3 peak shape looked bad. Replaced color reagent and both reagent pump tubes. Restarted, shape was improved but then NH4 peaks began to look bad. Noticed orange crud had built up in block. Ran 1 M HCl through and left in lines overnight. Will finish run tomorrow, was only able to complete 36 samples. Acid wash dishes.-Cathy

Acid wash dishes, prepare table for tomorrow\’s run, will be adding 41 samples for Lau lab. Made fresh Citrate reagent–last week the response values were lower than average and will check tomorrow if reagent prepared 7-15-2014 was the issue-Cathy Prepared Citrate reagent (2L): 63 grams sodium citrate #4960 JT Baker 2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 2 liters, filter-Cathy

Alpkem run, LTER 2013 soil (20130701.rst). Acid wash dishes-Cathy Check data-Erica

Alpkem run, 20130617.rst, LTER soil. Some samples for Tomomi (Lau lab) were added at the end of this run-Cathy Prepared Citrate reagent (2L): 63 grams sodium citrate #4960 JT Baker 2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 2 liters, filter-Cathy

Prepare fresh standards and table for tomorrow\’s run, acid wash dishes-Cathy

Alpkem run, LTER soil samples 6/6/2013-Cathy Acid wash dishes-Cathy

Attempted to run Chinchilla but flow cell holder was not sent with the flow cell and instrument in not useable-Cathy Acid wash dishes-Cathy

Alpkem run, 2013522B.rst, 2nd half of yesterday\’s run-Cathy Acid wash dishes-Cathy

Alpkem run, LTER soil 5/22/2013. Because of problems last week with NO3 channel: filtered Ammonium Chloride solution a 2nd time. Seems to be working well now. NH4 sync peak was way too small. Made fresh sodium nitroferricyanide solution-no change. Made fresh alkaline phenol solution-no change. Remade hypochlorite solution with different bleach and peak looked fine. The \”splashless\” Clorox bleach was apparently the problem. Had time to run only half, will run remainder tomorrow -Cathy Prepared fresh Nitroferricyanide Reagent, two batches of 1 L each: Sodium Nitroferricyanide: 0.5g lot # H47599. Nanopure water to 1L, filter. -Cathy Acid wash dishes-Cathy

Prepare fresh standards, acid wash dishes-Cathy

Acid wash dishes. Solution not moving well through NO3 channel, seems blocked. Removed NO3 block from Alpkem and flushed with water. Ran Brij/H2O then NaOH then brij/H2O. Seems to be pumping through block better after cleaning.-Cathy Ran standards on Chinchilla with new (older model) flow cell-Cathy

Alpkem run, 20130507. Running some of these samples on Chinchilla today as well (1-1 through 7-3).-Cathy Acid wash dishes-Cathy

Alpkem run, 2013424B.rst. These are the samples from 20130424 that had failing CCVs last week. Started with 3-3a–samples run prior to this this were bracketed by passing CCVs. Run went well, all passed. Added more samples at end of run from 20130424 that had barely passing CCVs to see if they could be improved, which they were but sample results were similar. Only results 1-1 through 5-2 will be reported from 20130424.rst. The remaining results will be reported from 2013424b.rst-Cathy Acid wash dishes-Cathy

Changed all pump tubing, cleaned both flow cells, acid washed brij/H2O container-Cathy

Alpkem run, 20130424.rst. CCVs failing in middle of run and needed to abort run, will investigate furthur on Friday. Acid wash dishes-Cathy Test run of 0.2ppm stds and blanks on Chinchilla. Downloaded software to enable printing .slz files on lab computer-Cathy

Chinchilla runs. Running LTER samples from 4/24/2013.-Cathy

Alpkem run, LTER 2013 4/10/2013 (20130410.rst)-Cathy Replaced NO3 sample pump tube.

Received new brij and started run using this but peak shape for NO3 was not good. Looks like some kind of contamination. Prepared fresh Ammonium Chloride reagent, will retry tomorrow Replaced tubing from probe to \”T\” and from \”T\” to NO3 sample pump tube.-Cathy Prepared Citrate reagent (2L): 63 grams sodium citrate #4960 JT Baker 2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 2 liters, filter-Cathy Acid wash dishes-Cathy Prepared Ammonium Chloride Reagent (2L): 170 gms Ammonium Chloride LOT# 1702C514 BDH 0.2 gms EDTA Lot # 083759A Approximately 15mL Sodium Hydroxide Lot # 51748, pH to 8.53, filter-Cathy

Attempted Alpkem run..CCV values are still failing on both channels; all standards, ICV and first CCV are good then first CCV drops dramatically and third CCV fails. Called Tim Smith at OI Analytical–he is convinced that the brij (TX-10) is bad and is sending a bottle of brij tomorrow.-Cathy

Attempted to run Alpkem but second CCVs failing on both channels and peak height on NO3 sync peak was low and carryover for NO3 was 1.0. (standards, ICV and first CCV were fine) Cleaned out KCl container, there was a little black moldy stuff in some of the lines. Cleaned tubing piece at top of probe, also a little dirty. Prepared fresh Ammonium Chloride solution and Color Reagent. Cleaned NO3 channel with NaOH and NH4 channel with HCl. -Cathy Prepared Ammonium Chloride Reagent (2L): 170 gms Ammonium Chloride LOT# 1702C514 BDH 0.2 gms EDTA Lot # 083759A Approximately 15mL Sodium Hydroxide Lot # 51748, pH to 8.48, filter-Cathy Prepared Color Reagent (1L): 100 mL Phosphoric Acid lot # 31737 40 grams Sulfanilimide, lot # SLBD9423V-NEW LOT 2 grams N-1-Naphthylethylenediamine dihydrochloride, lot # MKBJ8972V Nanopure to 1 L, filter.-Cathy

Prepare standards and table for tomorrow\’s Alpkem run, replace NO3 sample and wash tower waste pump tubes, cleaned out sampler probe chamber, acid wash dishes.-Cathy

Alpkem run, rerunning last 36 samples from yesterday, CIMMYT series 45. Recharged coil prior to run. Acid wash dishes.-Cathy

Alpkem run, CIMMYT series 45. Failing CCVs toward end of run. Will rerun last 36 samples tomorrow and also recharge column before starting run.-Cathy

Alpkem run, CIMMYT series 44-Cathy

Prepared fresh Nitroferricyanide Reagent (1L): Sodium Nitroferricyanide: 0.5g lot # H47599. Nanopure water to 1L, filter.-Cathy Acid wash dishes, lab cleanup-Cathy

Alpkem run, CIMMYT series 42 and 43 (CIMM4243.rst)-Cathy

Alpkem run, CIMMYT samples, series 40 and 41 (CIMM4041.rst). Acid wash dishes, replaced NO3 pump tube.-Cathy

Acid wash dishes, organize CIMMYT samples, set up table for tomorrow\’s run.-Cathy

Alpkem run, CIMMYT39.rst-Cathy Prepared Citrate reagent (2L): 63 grams sodium citrate #4960 JT Baker 2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 2 liters, filter-Cathy

Alpkem run, CIMMYT38.rst. Acid wash dishes. Prepared Ammonium Chloride Reagent (4L): 340 gms Ammonium Chloride LOT# 1702C514 BDH 0.4 gms EDTA Lot # 083759A Approximately 35mL Sodium Hydroxide Lot # 51748, pH to 8.50, filter-Cathy

Alpkem run, CIMMYT series 37. Prepare fresh standards.-Cathy

Alpkem run, CIMMYT36.rst, acid wash dishes, replaced several pump tubes, recharged coil prior to running-Cathy Prepared fresh color reagent: 100 mL Phosphoric Acid lot # 31737 40 grams Sulfanilimide, lot # SLBD9423V-NEW LOT 2 grams N-1-Naphthylethylenediamine dihydrochloride, lot # MKBJ8972V Nanopure to 1 L, filter.-Cathy

GC run, CIMMYT35.rst.

Alpkem run, CIMMYT34.rst. NO2 was high which made efficiency too low; reprepped NO2 standard, restarted and coil efficiency passed but ICV was low for NH4. Remade ICV, started again, all okay this time. Acid wash dishes.-Cathy

Acid wash dishes, prepare fresh standards, organize samples and prepare table for tomorrow\’s run.-Cathy Prepared Ammonium Chloride Reagent (2L): 170 gms Ammonium Chloride LOT# 1702C514 BDH 0.2 gms EDTA Lot # 083759A Approximately 15mL Sodium Hydroxide Lot # 51748, pH to 8.49, filter-Cathy

Alpkem run, CIMMYT series 33. Acid wash dishes.-Cathy

Alpkem run, CIMMYT series 32. Acid wash dishes..-Cathy

Upload 2013 GLBRC N data.-Cathy

Alpkem run, GLBRC 2013 Marshall 11/11/2013.

Alpkem run, GLBRC Lux Arbor, 11-11-2013. Check and upload data, acid wash dishes, cleaned pump rollers with Brasso, changed color reagent and NH4 sample pump tubes, prepared 1 L Sodium Nitroferricyanide.-Cathy

Prepare fresh standards, acid wash dishes, filter KCl. Data checking and uploading.-Cathy

Alpkem run, Organic Transitions 11/20/2013, 2nd box, NH4 only. Used citrate buffer prepared yesterday although NH4 lines look much cleaner after leaving in 1 N HCl overnight and this was most likely the main problem yesterday. Made Excel sheet indicating which boxes correspond to each result file name, stored in OrgTrans 2013 file, emailed Organic Transition people and let them know their samples are finished-Cathy Acid wash dishes, prepared fresh 1 M KCl (25L)-Cathy

Alpkem run, Organic Transitions 11/20/2013. Peak shape on NH4 channel not good. Noticed that Citrate buffer was not decreasing at normal rate and found some debris? in the pump tube. Paused run, changed pump tube. In the process NH4 sample pump tube platen came loose and drew some air into system affecting some NO3 samples as well. CCVs are still too low for NH4. This is the first run that citrate buffer prepared 3/12 was used..contaminated? Prepared 1 L of fresh citrate buffer. Re injecting affected NO3 samples and will rerun all for NH4 tomorrow. Ran 1 N HCl through NH4 lines and left overnight. -Cathy

Alpkem run, OrgTrans 11/20/2013 2nd box. Could not get coil efficiency above 90% even after making new NO2 standard. After three starts stopped run–there are 192 samples and there was not enough time to finish. Made all new standards and recharged coil. Will run samples Monday. Acid wash dishes.-Cathy Prepared Citrate reagent (2L): 63 grams sodium citrate #4960 JT Baker 2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. Nanopure water to 2 liters, filter-Cathy

Alpkem run, OrgTrans, 11/20/2013-Cathy

Prepared fresh standards, filter KCl, acid wash dishes-Cathy

Prepared fresh Nitroferricyanide Reagent (1L):

Sodium Nitroferricyanide: 0.5g lot # H47599.

Nanopure water to 1L, filter.-Cathy

2-28-2014 Alpkem

Alpkem run, OrgTrans samples 10/8/2014, T0 box #2 (13108t0b.rst).  Acid wash dishes.-Cathy

2-19-2014 Alpkem

Alpkem run, Org Trans samples, 1372228B.rst, 7/22/2013 T28 incubation, box 2.-Cathy

Replaced lamp in Channel #1, acid wash dishes.

Prepared Citrate reagent (2L):

63 grams sodium citrate  #4960 JT Baker

2 grams sodium hydroxide pellets, Macron Brand, lot # 52566.

Nanopure water to 2 liters, filter-Cathy

2-17-2014 Alpkem

Alpkem run, OrgTrans 7/22/13 T28 incubations (13722t28.rst).  Cleaned Ch #2 lines with 1 N NaOH-Cathy

2-4-2014 Alpkem

Alpkem run, OrgTrans samples, 5/6/13 T28 incubations box 2.  Left 1 N HCl in channel #1 overnight and serial communication values are improved–back to 50% @ 8 SAM and 72% @ 4 REF.  Spoke to Tim Smith yesterday who suggested I replace the lamp anyway since it has been a while and we use this instrument so frequently.  Acid wash dishes, replaced wash supply pump tube-Cathy

2-11-2014 Alpkem

Alpkem run, OrgTrans samples  7/22/2013 T0 Incubations, 2nd box (137220ib.rst).  The % light for the sample on channel 1 has been reading just above 50% and the gain has been 8, ref gain has been 4 so ratio 2 to1.  Today the % light went up to 98 % and the gain went up to 16.  So the sam to ref ratio is now 4 to 1.  Ordered a new lamp for replacement.-Cathy

Acid wash dishes-Cathy

2-10-2014 Alpkem

Prepare fresh 1 M KCl, acid wash dishes, ordering reagents, clean hood, tag and request ORCBS waste pickup.-Cathy

Prepared Ammonium Chloride Reagent (4L):

340 gms Ammonium Chloride LOT# 1702C514 BDH  Note:  this particular brand seems much dirtier than others.  Had to change filters when filtering because it was getting so dirty.

0.4 gms EDTA  Lot #  083759A

Approximately 35mL Sodium Hydroxide Lot # K31507, pH to 8.51, filter-Cathy

2-5-2013 Alpkem

Alpkem run, OrgTrans samples, 7/22/2013 T0 incubations-Cathy

Acid wash dishes-Cathy

Prepared fresh Nitroferricyanide Reagent (1 L):

Sodium Nitroferricyanide: 0.5g lot # H47599.

Nanopure water to 1 L, filter.

2-3-2014 Alpkem

Alpkem run, OrgTrans samples, 5/6/2013, T28 incubations (1356T28i.rst)-Cathy

1-29-2014 Alpkem

Alpkem run, OrgTrans samples, T0 incubations 5/6/2013, 2nd box. (1356t0iB.rst).  Acid wash dishes-Cathy

Prepared fresh color reagent:

100 mL Phosphoric Acid lot # 31737

40 grams Sulfanilimide,  lot # SLBC0284V

2 grams N-1-Naphthylethylenediamine dihydrochloride, lot # MKBJ8972V

Nanopure to 1 L, filter.-Cathy

1-28-2014 Alpkem

Prepare fresh standards, prepare table for tomorrow\’s run, acid wash dishes-Cathy

1-27-2014 Alpkem

Prepared Citrate reagent (2L):

63 grams sodium citrate  #4960 JT Baker

2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. 

Nanopure water to 2 liters, filter-Cathy

Acid wash dishes-Cathy

1-20-2014 Alpkem

Alpkem run, Org Trans samples, 5/6/2013 incubation T:0, (1356T0i.rst).  Replaced both debubbler pump tubes prior to starting run.-Cathy

1-15-2014 Alpkem

Alpkem run, Org Trans samples, 4/22/2013 second box.  (2013422B.rst).  Acid wash dishes.-Cathy

1-14-2014 Alpkem

Alpkem run, Organic Transition samples, 4-22-2013, (20130422.rst).  Changed alkaline phenol pump tube.  Acid wash dishes.-Cathy

1-13-2014 Alpkem

Prepared Ammonium Chloride Reagent (4L):

340 gms Ammonium Chloride [[[[[[[LOT]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}# 1702C514) (BDH brand-new lot)

0.4 gms [[[[[[[[[[[[EDTA]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}  Lot #  083759A

Approximately 35mL Sodium Hydroxide Lot # K31507, pH to 8.53, filter-Cathy

1-8-2014 Alpkem

Prepared fresh Nitroferricyanide Reagent (1 L):

Sodium Nitroferricyanide: 0.5g lot # H47599.

Nanopure water to 1 L, filter.  -Cathy

Uploading Alpkem data.  Discovered there is a 20 character limit for sample names in the Alpkem software.  Even though the sample table will accept more, the name will get cut off in the result file. 20130708G1R1mp-10-a is the tentative replacement for microplot samples (see HipChat 12-18-2013 approx. 1:50 pm.).

12-18-2013 Alpkem

Prepared fresh Nitroferricyanide Reagent (1 L):

Sodium Nitroferricyanide: 0.5g lot # H47599.

Nanopure water to 1 L, filter.  -Cathy

Acid wash dishes-Cathy

12-18-2013 Alpkem

Alpkem run, 2013 GLBRC soil, Lux Arbor, 5/15/2013 (13515LUX.rst). Acid wash dishes-Cathy

12-17-2013 Alpkem

Alpkem run, GLBRC 2013 soil samples, Marshall plots, 15-May-2013.(13515mar.rst)-Cathy

12-16-2013 Alpkem

Alpkem run, GLBRC 2013 soil, microplot incubations, 10/7/2013 (13107MI.rst)  Noticed some orange build-up in the NH4 channel lines.  Disconnected from flow cell, turned off heater and ran 3 M HCl through lines.  Also removed debubbler and soaked in 1 M HCl.  Reassembled and rinsed thoroughly with brij/H2O solution-Cathy

Acid wash dishes-Cathy

12-11-2013 Alpkem

Alpkem run, GLBRC 2013 soil samples, 10/7/2013 microplots (2013107M.rst)-Cathy

Acid wash dishes-Cathy

12-10-2013 Alpkem

Alpkem run, 2013 GLBRC soil samples, mainsite incubations (2013107I.rst).  Prepare table for tomorrow\’s run.-Cathy

Prepared Citrate reagent (2L):

63 grams sodium citrate  #4960 JT Baker

2 grams sodium hydroxide pellets, Macron Brand, lot # 52566. 

Nanopure water to 2 liters, filter-Cathy

Acid wash dishes-Cathy

12-9-2013 Alpkem

Alpkem run, GLBRC 2013 soil samples, mainsite.  (20131007.rst).  Acid wash dishes.

Prepared fresh color reagent:

100 mL Phosphoric Acid lot # 31737

40 grams Sulfanilimide,  lot # SLBC0284V

2 grams N-1-Naphthylethylenediamine dihydrochloride, lot # MKBJ8972V

Nanopure to 1 liter, filter. -Cathy

12-3-2013 Alpkem

Alpkem run, 2nd half of 2012 resource gradient samples.  Changed color reagent pump tube.  Prepared fresh standards, acid wash dishes, built table for tomorrow\’s run.-Cathy

12-2-2013 Alpkem

Build table for tomorrow\’s run (2012 resource gradient)

Prepared Ammonium Chloride Reagent (4L):

340 gms Ammonium Chloride [[[[[[[LOT]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}# slbg9946v) (Sigma brand)

0.4 gms [[[[[[[[[[[[EDTA]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}]{.caps}  Lot #  083759A

Approximately 35-40mL Sodium Hydroxide Lot # K31507, pH to 8.52, filter-Cathy

11-25-2013 Alpkem