Projectlog Entries List

BCSE Series 2 gas sampling - Jon, Emily BCSE Hydrosense - Alyssa

Fix LTAR and GLBRC station flags - Olivia

Dishes - Jon, Emily

GC Sequence LTER20260401S2BGC had some vials submitted out of order in the field trays. All samples were run in sequential order on the GC. Vials submitted out of order were as follows: …148, 150, 149, 151, 152… and …241, 242 ,247, 244, 245, 250, 251, 248, 249, 246, 243, 252, 253…

LTAR Series 4 gas sampling - Olivia, Jon, Brian, Emily LTAR Hydrosense - Alyssa

Install and fix LTER and LTAR station flags - Jon, Emily

Dishes - Brian

BCSE and LTAR gas frame prep. All LTAR gas frames given colored flags that match gas sampling grouping - Sarah, Brian

Collect LTAR Lysimeters and O-rings from field (to be washed).

BCSE and LTAR gas vial and tote prep - Brian, Sarah

LTER soil and LTAR Lysimeter filtering - Brian, Emily, Jon

Prepared 1M KCL, standards.

Lachat run LTER Lysimeter 20250506. -Sample DF3-1 was missing from set. Vial DF3-3 should not exist, ran as sample DF3-1.

Evacuate LTAR Lysimeters - Jon, Brian

Forest Maintenance - Brian, Emily

Help move OTC’s in T7- Jon

LTER Soil extraction - Emily, Olivia

Dishes- Jon

LTER 1st April soil sampling and sieving - Alyssa, Jon, Emily, Brian, Sarah.

Collect LTER Lysimeters - Jon AM/PM, Emily PM

Install LTER station flags - Emily AM, Brian AM/PM, Olivia PM

GC Sequence LTAR202603250402S2S3BGC had one set of sample vials out of order, as submitted by Techs. Vials were in no discernable order for that last rack so recorded in this order: 189,190,191,172,169,186,187,176,173,182,183,180,177,178,179,184,181,174,175,188,185,170, 171,192. Could be read in the opposite order as well? Anyhow, all samples run on GC in sequential order. Will look to see if this rack simply got jumbled up in the transport from field to lab.

Weigh and filter LTER Lysimeters - Jon, Emily

Acid washed dishes.

Lachat run LTER Lysimeter 20250421. Run notes:

• Samples T1 R2-2 and T7 R2-1 should have samples, but no vial was in sampled box, skipped.
• Sample CF1-1 has no vial, should exist. Vial labeled as CF1-6 does not have enough volume listed to filter, ran as CF1-1.

LTER Series 2 gas sampling - Emily, Brian, Sarah, Jon, Alyssa/Stacey

LTER Hydrosense - Alyssa

Filter LTAR soil extracts - Jon

Prepared 1M KCL, Lachat run LTER Lysimeter 20250408.

Replaced end filter and 4th cartridge filter on Barnstead nanopure. Ran water test on Lachat, filter seems to be functioning normally again.

Weigh LTAR soil for extraction and moisture - Brian, Olivia

Prep LTER, LTAR gas vials - Emily

Prep LTER gas chambers, install T7, T7 micro and T8. T7 R1,3,4 microplot chambers were installed too far north and outside the microplot - had to be reinstalled just prior to sampling 4/1) - Emily, Jon

Prep LTAR gas chambers, reinstall ASP4, ASP5 and installed for the first time ASP2, ASP4, and ASP5 noNI subplot chambers. New chambers were measured for installation: 20m from the south side and 3m east or west from the plot edge (opposite side from the main plot chamber) - Jon, Brian

Burn all six T7 plots and former CE study - Stacey, Kevin, Sven, Joe, Ethan, Jarrod, Mark, Hsunyi, YJ, Alyssa, Emily, Brian, Jon, Elaine, Lucas, Erica, Holly

LTAR March soil sampling. No longer sampling R5 plots (may resume later this year). First time sampling no Nitrification Inhibitor (no NI) subplots in ASP2, ASP4, ASP5, these are on the opposite side of the plot from the main plot gas chambers, 15 feet wide. In the noNI subplots, six cores were taken in roughly the center of the subplot strip and in line EW with station 1,2,3, two cores per “station”, one in the row and one between. In the main plot station 1 and 2 were sampled NW of the station while station 3 was sampled east of the station to avoid the noNI subplots. - Alyssa, Brian, Jon, Emily

Uploaded GLBRC MLE MC (Lake City) 20251125 soil samples. We used the re-extracted G11 soil samples in place of the original samples due to possible incorrectly labeled vials.

All 2025 LTER, LTAR, and GLBRC Lachat soil run uploaded.

Sieve LTAR soil - Alyssa, Brian, Jon, Emily

Measure and filter LTAR leachate; dishes - Brian, Olivia

Collect LTAR leachate - Brian, Alyssa

Evacuate LTAR lysimeters - Olivia, Brian

Reflag LTAR plots with GPS - Brian

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Finished GLBRC Microplot soil 20251203 Lachat run and Re-extracted GLBRC MLE MC (Lake City) soil 20251125.

LTAR Series 2 gas sampling - Brian, Sarah, Stacey/Alyssa

LTAR Hydrosense - Alyssa

Filter LTER soil extracts - Brian, Sarah

Two heat flux plates were installed upside down on tower 2. White dot needs to face UP. I did not explain to Dave. They have probably been like this for several winter months.

Checked on towers 1 and 2 today.

Lachat run GLBRC Microplot Soil 20251203.

• unable to finish end of run, will continue on 3/26/26 (not uploaded).

LTAR gas chamber prep - Jon

Weigh LTER soil for extraction and moisture - Olivia, Brian

LTAR gas vial prep - Jon

Dishes - Brian, Jon

Prepared 1m KCL. Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

March LTER soil sampling, NW of the stations (no station flags in T1,6,8 - locations approximated) - Alyssa, Brian, Sarah, Jon, Stacey

Sieve March LTER soil - Alyssa, Sarah, Brian, Jon, Stacey

Collect LTER leachate - Jon, Brian

Measure and filter LTER leachate - Jon, Brian

Lachat run GLBRC soil Main plots 20251201.

Evacuate LTER lysimeters - Jon, Brian

Flag forest paths, fix border PVC - Jon, Brian

Prepared standards, Lachat run LTAR soil 20251208.

• No vial ASP5 R2 a, missing from box. Ran as blank.

Grind SBE plants - Olivia

Grind SBE plants - Brian

Pack LTAR 2023 plants - Jon

Dishes, lab cleaning - Sarah

Lachat run GLBRC MLE WC soil 20251119 (Hancock).

Grind SBE plants - Jon

Pack LTAR 2023 plants - Brian

Safety training - Brian, Jon, Sarah

Fall project wrap-up - Sarah

Lab cleaning - Alyssa, Stacey

GC Sequence LTAR20260312S1BGC had a few samples submitted out of order. All samples run in numerical sequence on GC. Samples Dup3 and Dup4 were swapped with vials 3 and 4 as submitted. They were swapped back in proper order on GC run. Another sample out of order towards the end as such: ….112, 116, 113, 114, 115, 117, 118….

Lachat run GLBRC MLE MC soil 20251125 (Lake City). Notes from run: -No G8R3 N c sample, spilled when filtering, ran as blank.

• G6R3 N b and G6R3 S b samples may have swapped - ran in vial order.
• Many G11 samples may have been labeled incorrectly. G11 Samples to be reextracted to cross check on correct grouping and vial order. Not uploaded for now.

Pack LTAR 2023 CN - Olivia

Dishes - Jon, Olivia

Grind SBE 2025 - Brian

Relabel 2019, 2021 BCSE G2 harvest archive jars - Jon

Inventory LTER leachate archive - Brian

LTAR Series 1 gas sampling - Olivia, Jon, Brian

Dump leftover plant samples (researcher stored samples, LTAR 2025) - Olivia, Jon

Lachat run GLBRC Soil MLE WN 2025118 (Rhinelander)

• G6R2 E a and G6R2 W a vials may have been swapped, ran in vial box order.

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Dishes, grind SBE palnts, inventory LTER leachate samples - Sarah, Jon, Brian

Collect LTAR leachate - Brian, Jon

Prepared Standards, Lachat run GLBRC MLE MN soil 20250123 (Escanaba)

Grind SBE plants - Olivia, Brian

Measure and filter LTAR leachate, save archive sample, dishes - Olivia, Jon

Evacuate LTAR lysimeters - Brian, Jon

Dump soil, clean up field sites, install G1 station flags - Sarah, Brian

Grind SWF plants - Sarah, Jon

Lachat run GLBRC MLE MS soil 20251118 (Lux Arbor).

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER 20251117.

Grind LTAR 2025 plants - Sarah, Jon

One last check on LTER T7 microplot plant archive samples where ANPP records don’t match Archive records - Jon

Pack LTAR 2023 plant CN - Olivia

Lachat run LTER soil 20251015.

Check LTAR gas chambers, pull out ASP3 no compost chambers and install them in ASP2 no compost microplots based on GPS file - Jon, Olivia

Grind LTAR 2025 plants - Sarah

Pack LTAR 2023 plant CN - Jon

Prep LTAR gas vials - Olivia, Alyssa

Grind LTAR plants - Sarah

LTER Series 1 gas sampling (last winter sampling) - Jon, Alyssa, Dave

GC sequence BCSE20260303S1BGC notes: All samples run in sequential order. One set submitted out of order in trays as follows: vial # ….44, 45, 47, 46, 48, 49….

BCSE Series 1 2026 gas sampling - Jon, Olivia

Acid washed dishes, Replaced Cadmium column.

Prep LTER gas vials, grind LTAR plants - Jon

Prep BCSE gas vials and totes - Jon

Check BCSE and LTER gas chambers - Jon

Prepared standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTER soil 20250820 (Re-extracted on 2/24/2026 due to KCL contamination)

• 1 bin of soil bags from set was in a malfunctioning walk-in cooler (90 degrees). Noted below are those bags: T4 R1-R3, R5, T5 R1-R6, T6 R1-R6, T7 R1-R6

Grind LTAR 2025 plants - Brian, Jon

Pack LTAR 2023 plant CN - Jon

Dump finished (nitrate and ammonium uploaded and checked) soil samples -

Pull G8 flags for tree harvest soon, dump soil samples - Olivia, Brian

Grind LTAR 2025 plants - Olivia

Pack LTAR 2023 plant CN - Brian

Relabel BCSE 2021 archive jars - Jon

Filter 8/20 soil extracts, dishes - Jon, Brian

Prepared 1m KCL

Lachat run LTER soil 20250806 (Re-extracted on 2/23/26 to compare to contaminated samples)

Grind LTAR 2025 plants - Jon

Reweigh LTER 8/20/2025 soil samples for extraction and moisture, initial results were good but reextracted to compare to other ammonium contaminated samples. Also, some of this soil warmed up when a walk-in stopped working, so interesting to compare initial to warmed samples. - Brian, Sarah

Relabel BCSE 2021 archive jars - Brian

Weigh dry soil tins, wash dishes - Sarah

Grind LTAR 2025 plants - Brian

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Jon

Filter 8/6 soil extracts - Jon, Brian

Lachat run LTER T4 soil 20250723 (Re-extracted on 2/18/26 due to KCL contamination)

Grind LTAR 2025 plants - Jon, Sarah

Reweigh LTER 8/6/2025 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts, results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Brian, Sarah

Relabel BCSE 2019, 2021 archive jars - Jon

Weigh dry soil tins, wash dishes - Brian

Prepared 1M KCL

Lachat run LTER T3 soil 20250723 (Re-extracted on 2/18/2026 due to KCl contamination)

Reminder, tower two has only two sets of ground sensors. Will replace all but the heat flux plates this spring.

Reinserted all heat flux plates, temp sensors, and CS650s. Tower 1 has one CS650 with a chewed cable near the sensor.

Tipping bucket on tower 2 was not fully horizontal. There was one tip during the repair.

Acid washed dishes, reconditioned cadmium column.

Lachat run LTAR 20251103.

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Brian

Prepared Standards, Replaced all reagent tubing.

Lachat run LTAR 20251008.

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Brian

Filter 7/23/2025 soil extracts - Jon, Brian

Grind LTAR 2025 plants - Sarah

Reweigh LTER 7/23/2025 T3, T4 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts; results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Jon, Brian

Lachat run LTER T4 soil 20250929.

Prepared 1M KCL.

Dumped leftover BCSE 2025 plants, then started dumping finished soil samples - Brian, Sarah

Finish grinding BCSE 2025 plants, start grinding LTAR 2025 plants - Olivia, Brian

Pack LTAR 2023 plant CN - Jon

Cleaning at field lab and trucks, start relabeling BCSE 2019 plant archive jars with labels Nick Thrower sent with vertical barcodes so scanner can read them (they were horizontal and went around the corner of the jars, so couldn’t be read by a scanner) - Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER T3 Soil 20250929.

Finish dumping LTER leftover plants - Sarah

Acid washed dishes. Lachat run LTER Soil 20250915.

Grind BCSE plants - Jon

Pack LTAR plant 2023 CN - Brian

Finish subsampling SWF plants for BAF, cleaning - Sarah

Grind BCSE plants - Brian

Finish packing LTER plant 2023 CN, start packing LTAR 2023 plants - Jon, Olivia

Pick up LTAR field samples from Josh, dump LTER leftover plants - Jon