Sieve MLE WC - Hancock soil, sampled 11/19/2025 - Jon

Filter LTER leachate - Alyssa

Filter WN soil extracts - Olivia, Brian

Prepared Standards, Lachat run LTAR Lysimeter 20250624.

Collect leaf litter from traps in T5, G8, CF, DF, SF. Pull traps from G8 as trees are bare. - Jon, Brian, Olivia

Pull T6 station flags - Jon

Sieve MLE WN - Rhinelander soil, sampled 11/18/2025. Weigh dry soil tins - Stacey

Weigh WN soil for extraction and moisture - Stacey, Alyssa

Adjusted the charge parameters on tower 1 to the default parameters for Sealed Lead Acid batteries. I added a battery to power the access point prior to programming the charge controller. While that allowed me to finish programing the controller, it still required a power cycle to restore power to the load.

Prepared 1M KCL. Finished dilutions from LTAR Lysimeter 20250610.

BCSE Series 22 gas sampling - Jon, Brian, Alyssa (Hydrosense)

Collect LTER leachate - Brian, Jon

Evacuate LTER lysimeters - Jon, Olivia

BCSE gas chamber prep - Brian

ASP tower 1 rain gauge tipping mechanism was not working. Spider web was preventing it from tipping. Repaired. Missed yesterdays light rain. Added camera to tower 2. made final adjustment of height (2m) and direction (N-S) on each tower KK DW

BCSE gas vial, tote prep - Brian

Filter LTER soil extracts - Jon, Brian

Lachat run LTAR Lysimeter 20250610.

Prepared 1M KCL, Lachat run LTAR Lysimeter 20250529.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Weigh LTER soil for moisture and nitrogen extraction - Brian, Jon

Pack CN - Brian

Dishes - Jon

LTER soil sampling - Alyssa, Brian, Olivia, Jon

GC Sequence LTAR20251113S28BGC had a few vials submitted out of order. All samples run sequentially on GC. Vials submitted out of order as follows: …62, 63, 68, 65, 66, 67, 64, 69, 70… Also, note on tape says vials 41 and 44 were swapped, though they were submitted in sequential order in racks.

Sieve LTER soil - Alyssa, Brian, Olivia, Jon

Reran Channel 2 and dilutions of Lachat run LTAR Lysimeter 20250513.

Sieved BCSE soil and set out to air-dry - Sarah

Sieved Sorghum Nitrogen Rate soil and subsampled to send to A&L for S1 analysis. - Slater, Jon

Filter LTAR leachate, dishes -

Replace BCSE then LTAR station flags - Olivia, Brian

Sample Sorghum Nitrogen Rate Experiment soil. Location 2: one core per plot, pooled by block, 4 total. Location 1: split into northern and southern halves, one core per plot, pooled by block, 8 total. - Slater, Jon

Sample BCSE soil for laboratory respiration incubation. G1,3,5,8,10, R1-3. Four cores/plot, one or two per station, alternating within and between crop row for G1, G3 and G8. Three cores/plot, one per station pooled for G5 and G10. - Sarah

Measure LTAR leachate - Slater, Brian

LTAR Series 28 gas sampling - Slater, Brian, Jon, Alyssa (Hydrosense)

Collect LTAR leachate - Slater, Brian

Replaced Cadmium Column and reconditioned.

Lachat run LTAR Lysimeter 20250513. Notes from run:

• Vial ASP2 R3 C not run, vial missing from box.

Issue with calibration curve/column in channel 2 results - rerun 11/14.

LTAR gas chamber prep - Olivia, Sarah

Evacuate LTAR lysimeters; replace BCSE station flags - Slater, Jon

LTAR gas vial, tote prep - Olivia, Sarah

Weigh dry tins, dishes - Sarah

Acid washed dishes, Prepared standards.

Lachat run LTAR Lysimeter 20250428. Notes from run:

• Vial ASP5 R2 N missing, used vial ASP5 R1 N that shouldn’t exist (mislabeled?)
• Vial BAU1 R1 C not run, missing from box.
• Vial BAU2 R3 N has no volume listed, mislabeled or volume missed? Ran as labeled.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTAR Lysimeter 20250415.

Put away clean, dry dishes, weigh remaining BCSE grain, grind SWF 2024 harvest plant samples - Jon

LTER Series 15 gas sampling - Alyssa, Slater, Jon, Olivia, Sarah

LTER Hydrosense pm - Alyssa

Finish sorting ASP2 plants - Sarah

Attempted to use the MSView application to increase the Target voltage on tower 1 from 14.2 to 14.6 and the float voltage from 13.2 to 13.7. I had lowered these values for the LI-Ion battery, but now that we have an AGM battery they should probably be set back. Unfortunately the Sunsaver cuts power when writing to the coils, so the network goes down after the first coil is written too. I rebooted the Sunsaver and the Target voltage was set but the float voltage was still at the old value.

I the future, I will need to provide an alternate power source for the tower router before attempting to set the parameters through the MSView application.

Finished dilutions from Lachat run LTAR Lysimeter 20250401.

Collect leaf litter from traps in T5, G8, CF, DF, SF; reinstall LTER gas chambers in T1-4, plus prairie strips - Brian, Slater, Jon, Olivia

Shell LTAR Inhibitor Study corn with Josh Dykstra - Sarah

Finished dilutions from Lachat run LTAR Lysimeter 20250318.

Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTAR Lysimeter 20250401. Notes from run:

• Vials BAU1 R2 S and BAU1 R4 S missing from box, not run.
• All bottles were collected after 48 hours instead of 24 due to storms.

Sort ASP2 plants, put LTAR 11/3/2025 soil out to air-dry - Slater, Brian, Jon

Sort ASP2 plants - Slater, Brian, Olivia

Finish BAF subsampling for 2024 BCSE peak plant CN - Sarah

Dishes - Jon

Filter LTAR extracts, dishes - Slater, Brian, Jon

Sample LTAR Inhibitor Study corn - Kevin, Dave, Sarah, Josh

Finished dilutions from Lachat run LTAR Lysimeter 20250303.

Lachat run LTAR Lysimeter 20250318. Notes from run:

• Vial ASP6R3 S missing, used Vial labeled ASP6R2 S (vial shouldn’t exist, weight under 20).
• Samples ASP3 R4 C,S not filtered, bottles were left in fridge and skipped. -Samples ASP1 R4 N,C,S not filtered, bottles left out on counter and skipped.

Reconditioned Column.

BAU towers raised for tillage at 12:30 today. No sensors yet on tower 4. Tower 2 CS650 sensor 4 chewed in half again.

Prepared 1M KCL.

Prep LTER gas chambers. After chambers were prepped, heard from Joe that gas sampling could not happen tomorrow due to lime applications planned for T1-4. - Slater, Brian

Pulled all T1-4, plus prairie strip, gas chambers for lime. Finished clipping ASP2 plants. - Alyssa, Slater, Brian, Jon

Weigh LTAR soil for extraction and moisture - Alyssa, Jon

Prep LTER gas vials, totes - Alyssa, Jon, Brian

Sort ASP2 plants - Alyssa, Slater, Brian, Jon

Prepared Standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTAR Lysimeter 20250303. Notes from run:

• Vial ASP5R1C not run, vial was nearly empty and not enough to run.
• Vials ASP2R4 N,C,S and ASP6R4 N,S were evacuated on 3/4/2025 due to lid frozen shut.

Sieve and subsample LTAR soil for A&L - Jon, Brian, Olivia, Sarah

Weigh dry soil tins, dishes - Slater

LTAR November soil sampling, 4 cores/station, 12 cores total to ensure enough soil for both A&L analysis and air-dried archive besides extraction and moisture - Slater, Jon, Brian, Olivia, Sarah

Pull ASP6 flags and chambers for harvest

Start ASP2 plant sampling; Stations 1, 2, 3: 2mS, 0.5mW to NE corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. R5: Split the plot into quarters. Place the frame approximately in the center of the western most quarter (label west) and the eastern most quarter (label east). The frame should be perpendicular to forage rows, if visible. Sorted later in the field lab to MEDSA, TRFPR, CICSP, DACGL, BRSNA (live volunteer canola), and remainder UNSRT. - Jon, Brian, Olivia, Sarah

BCSE20251023S21BGC sequence notes: All samples run in sequential vial number order. Samples submitted out of order in trays as follows: …59, 60, 61, 78, 79, 64, 65, 74, 75, 68, 69, 70, 71, 72, 73, 66, 67, 76, 77, 62, 63, 80…

Lachat run LTAR Soil 20250512.

Sieve SWF soil and put a portion in A&L soil bags to send for agronomic analysis. - Slater, Jon, Brian, Emily

Sort ASP1 plants - Ainsley, Olivia

Switchgrass Nitrogen Rate Experiment (SWF) soil sampling to send to A&L. Six cores/plot taken from the yield strip harvested area down the center of each plot, along a diagonal from NE to SW plot corners. - Brian, Slater, Jon, Emily

Lowered tower 1 to 2 m above the soil. Cleaned/unplugged rain gauge at 11:20ish. Reinstalled all ground sensors.

Weigh plants - Brian

Grind plants - Slater

Sort ASP1 plants - Alyssa, Ainsley, Brian, Emily, Jon, Slater

Fix on LTAR lysimeter bottles not yielding/not holding vacuum. Site BAU2, Rep 2 bottles checked - lines between bottle and prenart appear to be intact, line connection loose at bottle attachment fixed/sealed, o-rings reversed to proper positioning. Fixed O-rings and one pinched tube on ASP5, Rep 2. Lines appear to be intact between bottles and prenarts.

LTAR 27 gas sampling - Emily, Ainsley, Jon, Alyssa (Hydrosense)

ASP1 plant sampling ahead of mowing tomorrow, Stations 1, 2, 3: 1mS, 0.5mW to NE corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. R5: Place the frame approximately in the center of each half of the plot (east and west halves). Sorted in the field lab later… MEDSA, TRFPR, CICSP, DACGL, BRSNA (live volunteer canola), remainder UNSRT - Alyssa, Slater, Brian, Jon, Emily, Ainsley

Lachat run MDARD August 2025 Soil.

Sensors back in BAU1. Replaced soil moisture sensors and 3 heat flux plates. We need more 107s or 616s for soil temp.

LTAR gas vial, tote prep - Ainsley, Slater

Weigh plants - Brian

Filter MLE MN soil extracts - Ainsley, Jon

LTAR gas chamber prep - Ainsley, Slater

T7 post frost plant sampling, 2mS, 4mW of each station flag to the NE corner of 2x0.5m frame - Olivia, Sarah, Emily, Jon, Slater, Brian

Weigh MLE MN soil for extraction and moisture - Ainsley, Brian

Measure and filter LTER leachate - Alyssa, Emily

Pack CN - Ainsley

BAF subsampling for BCSE 2024 - Jon

Grind plants - Slater

Weigh plants - Brian

updated flux tower 2 program to reflect the new heat flux plate calibrations. The calibration coefficients for heat flux plate 1 and 3 needed to be reversed to match the way the probes were hooked up.

Collect LTER leachate - Alyssa, Slater

Help Levi Dreiling with barley sampling near the Rainout Building, field D2 - Emily, Jon

Evacuate LTER lysimeters - Jon, Emily

Pull G4, 5, 7, 9, 10 flags and frames for harvest - Olivia, Sarah

Help Mark move OTCs - Emily, Jon

Acid washed dishes, Lachat run LTAR Soil 20250414.

Dishes, weigh dry soil tins - Slater, Ainsley

Weigh plants - Sarah

Grind plants - Jon

Sieve MLE MN soil (sampled 10/23/2025) - Ainsley, Slater, Emily

Removed tower 1 ground sensors for harvest at 10:15AM. Looks like the rain gauge is completely full of water but too high (14.5’) to really tell..

Prepared Standards, Lachat run LTAR Soil 2025031.

Collect leaf litter from T5, G8, CF, DF, SF - Slater, Emily, Jon

Weigh plants - Jon, Ainsley

Pack CN - Ainsley, Slater

Replaced all Lachat reagent tubing.

BCSE 21 gas sampling - Emily, Jon, Alyssa (Hydrosense)

Weigh plants - Slater

Pack CN - Ainsley, Jon

Filter MLE MS soil extracts - Emily, Ainsley

LTAR20251016S26BGC GC sequence run had sample vials submitted out of order. All sample vials run on GC in numerical order. Submitted vials out of order as follows: …96, 97, 118, 119, 120, 101, 122, 123-144, 117, 98, 99, 100, 121, 102, 103-116.

Prepared 1M KCL

Collect LTAR leachate - Slater, Jon

BCSE gas chamber prep - Sarah

LTER20251010S14BGC samples submitted out of sequence. All sample vials run in numerical order, but they were submitted in this order: …161, 162, 167, 164, 165, 166, 163, 168, 169, 170… then again, …219, 220, 222, 221, 223, 224, 225…

Measure and filter LTAR leachate - Slater, Jon

BCSE gas vial, tote prep - Sarah

Weigh MLE MS soil for extraction and moisture - Ainsley, Emily

Weigh plants - Olivia, Ainsley

Pack CN - Slater

Data entry -

Evacuate LTAR lysimeters - Ainsley, Jon

Shell and shred G1 corn with Jarrod/Ethan operating the shredder - Slater, Emily

Weigh plants - Alyssa, Ainsley

Pack CN - Sarah, Alyssa

Sieve MLE MS soil (sampled 10/21 am by Todd and Chris) - Slater, Emily, Jon

Pack CN - Olivia, Jon

Weigh plants - Slater, Ainsley

Dishes - Slater

ChemInventory spreadsheet set up - Alyssa

Shell and shred LTAR corn with Josh Dykstra operating the shredder - Jon, Ainsley

Shell IP1 corn, no shredding/stover discarded per Brook’s instructions - Emily, Sarah

Dump soil - Sarah, Emily

Finish checking transect data - Slater

Weigh plants - Emily, Alyssa

Pack CN - Alyssa, Brian

Filter LTER soil extracts - Ainsley, Emily

Finish N-deposition fertilization, SF2, DF3, and CF3 - Brian, Ainsley

Wires trenched in at both new LTAR new towers. Waiting for sensors.

LTAR Series 26 gas sampling. When ASP3 (including no compost microplot) and BAU2 chambers were installed on 10/15/2025, the corn stover was removed for installation and not replaced. This was noted during sampling but stover wasn’t replaced inside those chambers until after sampling today. - Jon, Slater, Emily, Alyssa (Hydrosense)

Fertilize N-deposition microplots, finished CF1, CF2, DF1, DF2, SF1, and SF3 - Brian, Emily, Olivia

Weigh LTER soil for extraction and moisture - Olivia, Ainsley

Weigh plants - Brian

Transect data entry/checking - Ainsley, Alyssa

Pack CN - Slater, Stacey

Uploaded GLBRC Scale up Lux Arbor and Marshall samples in multiple data sets due to partial contamination. Notes are as follows:

L1 and L3 samples from the Lux Arbor Scale up field (12/2/2024) had Ammonia contamination in KCL used, so all ammonia results were discounted. L2 was sampled on different date (12/16/2024) and had no contamination, results will be uploaded in a separate file.

M1, M2 and M4 samples from the Marshall Scale up field (12/2/2024) had Ammonia contamination in KCL used, so all ammonia results were discounted. M3 was sampled on different date (12/16/2024) and had no contamination, results will be uploaded in a separate file.

Prepared 1M KCL

ASP Tower 2 and Bau tower 2 both infrastructures installed over the course of the week. Tower positions measured by Brook Wilke, with Kevin present. Sensors will come from Jiquan Chen.

Dropped BAU Tower 1 to standard 2m height after corn harvest. Will take some time to move ground sensors back as 8/9 destroyed by coyotes. Tower height was very easy to drop down.

LTER October soil sampling - Alyssa, Slater, Olivia, Brian, Ainsley, Jon

LTAR gas chamber prep - Emily, Sarah

LTAR gas vial, tote prep - Emily, Sarah

Weigh plants - Ainsley

Transect data entry/checking - Emily, Sarah, Alyssa, Olivia

Pack CN - Sarah, Stacey

Weigh dry soil tins, dishes - Brian

Sieve LTER soil - Slater, Emily, Jon

Transect data entry/checking - Brian, Ainsley, Emily, Slater

Weigh G1 corn - Alyssa

Dishes - Brian

Pack CN - Stacey

Thresh soybeans, canola - Emily, Slater, Alyssa, Ainsley

Pull chambers, flags from ASP3 and BAU2 for corn harvest - Alyssa

ASP3 corn sampling (same as BAU2): R1-4 all stations: 0.66mS, 0.66mW to NE corner of 1.52 x 0.66m frame, oriented with the 0.66m ends on the east and west. Perpendicular to corn rows. R5: Place the frame approximately in the center of each half of the plot (east and west halves), with the 0.66m frame ends oriented towards the north and south. - Slater, Brain, Olivia, Sarah, Emily, Ainsley

Innovations Plot 1 (IP1) Short Corn Trial sampling: Area is split into East and West halves. Each half includes the same three treatments: Short Corn, Control 1 and Control 2. Six samples will be taken from each treatment, three from the east (labeled south, center, north) and three from the west (labeled south, center, north). The IP1 field is 85m from south to north, same as ACSE plots. So, the same distances from the south end were used to match the station flag distances from the south end: south was about 12m from the south end, center about 45 m from the south end, and north about 64m from the south end. Per Brook’s instructions on the EW sampling locations, short corn was sampled near the center of the eastern half of each “rep”, otherwise when planted in 3 rows - rows 2 and 3 from the west were sampled and when planted in 6 rows - rows 5 and 6 from the west were sampled. Use 1.52 x 0.66m frame, oriented with the 0.66m ends on the east and west. Perpendicular to corn rows.- Slater, Brain, Olivia, Sarah, Emily, Ainsley, Alyssa

BAU Tower 79-2 ground sensors removed for harvest this morning. Found all sensors destroyed even after we covered them with corrugated cover. Coyote went right to the sensor and chewed off in all cases.

Filter LTAR soil extracts, dishes - Slater, Emily

Pack CN - Stacey

LTER Series 14 gas sampling - Brian, Jon, Ainsley, Slater, Emily

LTER Hydrosense - Alyssa

Weigh LTAR soil for extraction and moisture - Brian, Ainsley

LTER gas vial and tote prep - Slater

Transect data entry - Jon

Pack CN - Stacey

LTER gas chamber prep -

Collect leaf litter from traps in T5, G8, CF, DF, SF -

LTAR October soil sampling -

Thresh soybeans -

Transect data entry - Brian, Ainsley

Weigh plants - Sarah

Pack CN - Stacey

Pack CN - Stacey

Weigh plants - Brian, Jon

Plant species composition transect data entry - Emily, Slater, Ainsley, Brian

Weigh dry soil tins, dishes - Jon