Projectlog Entries List

GC Sequence LTAR202603250402S2S3BGC had one set of sample vials out of order, as submitted by Techs. Vials were in no discernable order for that last rack so recorded in this order: 189,190,191,172,169,186,187,176,173,182,183,180,177,178,179,184,181,174,175,188,185,170, 171,192. Could be read in the opposite order as well? Anyhow, all samples run on GC in sequential order. Will look to see if this rack simply got jumbled up in the transport from field to lab.

Filter LTAR soil extracts - Jon

LTER Series 2 gas sampling - Emily, Brian, Sarah, Jon, Alyssa/Stacey

LTER Hydrosense - Alyssa

Weigh LTAR soil for extraction and moisture - Brian, Olivia

Prep LTER, LTAR gas vials - Emily

Prep LTER gas chambers, install T7, T7 micro and T8. T7 R1,3,4 microplot chambers were installed too far north and outside the microplot - had to be reinstalled just prior to sampling 4/1) - Emily, Jon

Prep LTAR gas chambers, reinstall ASP4, ASP5 and installed for the first time ASP2, ASP4, and ASP5 noNI subplot chambers. New chambers were measured for installation: 20m from the south side and 3m east or west from the plot edge (opposite side from the main plot chamber) - Jon, Brian

Sieve LTAR soil - Alyssa, Brian, Jon, Emily

Uploaded GLBRC MLE MC (Lake City) 20251125 soil samples. We used the re-extracted G11 soil samples in place of the original samples due to possible incorrectly labeled vials.

All 2025 LTER, LTAR, and GLBRC Lachat soil run uploaded.

Burn all six T7 plots and former CE study - Stacey, Kevin, Sven, Joe, Ethan, Jarrod, Mark, Hsunyi, YJ, Alyssa, Emily, Brian, Jon, Elaine, Lucas, Erica, Holly

LTAR March soil sampling. No longer sampling R5 plots (may resume later this year). First time sampling no Nitrification Inhibitor (no NI) subplots in ASP2, ASP4, ASP5, these are on the opposite side of the plot from the main plot gas chambers, 15 feet wide. In the noNI subplots, six cores were taken in roughly the center of the subplot strip and in line EW with station 1,2,3, two cores per “station”, one in the row and one between. In the main plot station 1 and 2 were sampled NW of the station while station 3 was sampled east of the station to avoid the noNI subplots. - Alyssa, Brian, Jon, Emily

Collect LTAR leachate - Brian, Alyssa

Measure and filter LTAR leachate; dishes - Brian, Olivia

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Finished GLBRC Microplot soil 20251203 Lachat run and Re-extracted GLBRC MLE MC (Lake City) soil 20251125.

Evacuate LTAR lysimeters - Olivia, Brian

Reflag LTAR plots with GPS - Brian

LTAR Series 2 gas sampling - Brian, Sarah, Stacey/Alyssa

LTAR Hydrosense - Alyssa

Filter LTER soil extracts - Brian, Sarah

Weigh LTER soil for extraction and moisture - Olivia, Brian

LTAR gas vial prep - Jon

Dishes - Brian, Jon

Lachat run GLBRC Microplot Soil 20251203.

• unable to finish end of run, will continue on 3/26/26 (not uploaded).

LTAR gas chamber prep - Jon

Two heat flux plates were installed upside down on tower 2. White dot needs to face UP. I did not explain to Dave. They have probably been like this for several winter months.

Checked on towers 1 and 2 today.

Prepared 1m KCL. Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

March LTER soil sampling, NW of the stations (no station flags in T1,6,8 - locations approximated) - Alyssa, Brian, Sarah, Jon, Stacey

Sieve March LTER soil - Alyssa, Sarah, Brian, Jon, Stacey

Measure and filter LTER leachate - Jon, Brian

Lachat run GLBRC soil Main plots 20251201.

Collect LTER leachate - Jon, Brian

Prepared standards, Lachat run LTAR soil 20251208.

• No vial ASP5 R2 a, missing from box. Ran as blank.

Grind SBE plants - Olivia

Evacuate LTER lysimeters - Jon, Brian

Flag forest paths, fix border PVC - Jon, Brian

Grind SBE plants - Brian

Pack LTAR 2023 plants - Jon

Dishes, lab cleaning - Sarah

Lachat run GLBRC MLE WC soil 20251119 (Hancock).

Grind SBE plants - Jon

Pack LTAR 2023 plants - Brian

GC Sequence LTAR20260312S1BGC had a few samples submitted out of order. All samples run in numerical sequence on GC. Samples Dup3 and Dup4 were swapped with vials 3 and 4 as submitted. They were swapped back in proper order on GC run. Another sample out of order towards the end as such: ….112, 116, 113, 114, 115, 117, 118….

Safety training - Brian, Jon, Sarah

Fall project wrap-up - Sarah

Lab cleaning - Alyssa, Stacey

Lachat run GLBRC MLE MC soil 20251125 (Lake City). Notes from run: -No G8R3 N c sample, spilled when filtering, ran as blank.

• G6R3 N b and G6R3 S b samples may have swapped - ran in vial order.
• Many G11 samples may have been labeled incorrectly. G11 Samples to be reextracted to cross check on correct grouping and vial order. Not uploaded for now.

Pack LTAR 2023 CN - Olivia

Dishes - Jon, Olivia

Grind SBE 2025 - Brian

Relabel 2019, 2021 BCSE G2 harvest archive jars - Jon

LTAR Series 1 gas sampling - Olivia, Jon, Brian

Dump leftover plant samples (researcher stored samples, LTAR 2025) - Olivia, Jon

Inventory LTER leachate archive - Brian

Lachat run GLBRC Soil MLE WN 2025118 (Rhinelander)

• G6R2 E a and G6R2 W a vials may have been swapped, ran in vial box order.

Dishes, grind SBE palnts, inventory LTER leachate samples - Sarah, Jon, Brian

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Collect LTAR leachate - Brian, Jon

Grind SBE plants - Olivia, Brian

Measure and filter LTAR leachate, save archive sample, dishes - Olivia, Jon

Prepared Standards, Lachat run GLBRC MLE MN soil 20250123 (Escanaba)

Evacuate LTAR lysimeters - Brian, Jon

Dump soil, clean up field sites, install G1 station flags - Sarah, Brian

Lachat run GLBRC MLE MS soil 20251118 (Lux Arbor).

Grind SWF plants - Sarah, Jon

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER 20251117.

Grind LTAR 2025 plants - Sarah, Jon

One last check on LTER T7 microplot plant archive samples where ANPP records don’t match Archive records - Jon

Pack LTAR 2023 plant CN - Olivia

Grind LTAR 2025 plants - Sarah

Pack LTAR 2023 plant CN - Jon

Prep LTAR gas vials - Olivia, Alyssa

Lachat run LTER soil 20251015.

Check LTAR gas chambers, pull out ASP3 no compost chambers and install them in ASP2 no compost microplots based on GPS file - Jon, Olivia

LTER Series 1 gas sampling (last winter sampling) - Jon, Alyssa, Dave

Grind LTAR plants - Sarah

GC sequence BCSE20260303S1BGC notes: All samples run in sequential order. One set submitted out of order in trays as follows: vial # ….44, 45, 47, 46, 48, 49….

Prep LTER gas vials, grind LTAR plants - Jon

BCSE Series 1 2026 gas sampling - Jon, Olivia

Acid washed dishes, Replaced Cadmium column.

Prepared standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prep BCSE gas vials and totes - Jon

Lachat run LTER soil 20250820 (Re-extracted on 2/24/2026 due to KCL contamination)

• 1 bin of soil bags from set was in a malfunctioning walk-in cooler (90 degrees). Noted below are those bags: T4 R1-R3, R5, T5 R1-R6, T6 R1-R6, T7 R1-R6

Check BCSE and LTER gas chambers - Jon

Dump finished (nitrate and ammonium uploaded and checked) soil samples -

Grind LTAR 2025 plants - Brian, Jon

Pack LTAR 2023 plant CN - Jon

Pull G8 flags for tree harvest soon, dump soil samples - Olivia, Brian

Grind LTAR 2025 plants - Olivia

Pack LTAR 2023 plant CN - Brian

Relabel BCSE 2021 archive jars - Jon

Filter 8/20 soil extracts, dishes - Jon, Brian

Grind LTAR 2025 plants - Jon

Reweigh LTER 8/20/2025 soil samples for extraction and moisture, initial results were good but reextracted to compare to other ammonium contaminated samples. Also, some of this soil warmed up when a walk-in stopped working, so interesting to compare initial to warmed samples. - Brian, Sarah

Relabel BCSE 2021 archive jars - Brian

Weigh dry soil tins, wash dishes - Sarah

Prepared 1m KCL

Lachat run LTER soil 20250806 (Re-extracted on 2/23/26 to compare to contaminated samples)

Lachat run LTER T4 soil 20250723 (Re-extracted on 2/18/26 due to KCL contamination)

Grind LTAR 2025 plants - Brian

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Jon

Filter 8/6 soil extracts - Jon, Brian

Grind LTAR 2025 plants - Jon, Sarah

Reweigh LTER 8/6/2025 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts, results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Brian, Sarah

Relabel BCSE 2019, 2021 archive jars - Jon

Weigh dry soil tins, wash dishes - Brian

Prepared 1M KCL

Lachat run LTER T3 soil 20250723 (Re-extracted on 2/18/2026 due to KCl contamination)

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Brian

Acid washed dishes, reconditioned cadmium column.

Lachat run LTAR 20251103.

Reminder, tower two has only two sets of ground sensors. Will replace all but the heat flux plates this spring.

Reinserted all heat flux plates, temp sensors, and CS650s. Tower 1 has one CS650 with a chewed cable near the sensor.

Tipping bucket on tower 2 was not fully horizontal. There was one tip during the repair.

Prepared Standards, Replaced all reagent tubing.

Lachat run LTAR 20251008.

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Brian

Filter 7/23/2025 soil extracts - Jon, Brian

Dumped leftover BCSE 2025 plants, then started dumping finished soil samples - Brian, Sarah

Grind LTAR 2025 plants - Sarah

Reweigh LTER 7/23/2025 T3, T4 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts; results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Jon, Brian

Lachat run LTER T4 soil 20250929.

Prepared 1M KCL.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER T3 Soil 20250929.

Finish grinding BCSE 2025 plants, start grinding LTAR 2025 plants - Olivia, Brian

Pack LTAR 2023 plant CN - Jon

Cleaning at field lab and trucks, start relabeling BCSE 2019 plant archive jars with labels Nick Thrower sent with vertical barcodes so scanner can read them (they were horizontal and went around the corner of the jars, so couldn’t be read by a scanner) - Brian

Grind BCSE plants - Jon

Pack LTAR plant 2023 CN - Brian

Finish subsampling SWF plants for BAF, cleaning - Sarah

Acid washed dishes. Lachat run LTER Soil 20250915.

Finish dumping LTER leftover plants - Sarah

Grind BCSE plants - Brian

Finish packing LTER plant 2023 CN, start packing LTAR 2023 plants - Jon, Olivia

Lachat run LTER Soil 20250820 - KCL Contamination in Channel 1 blanks (NH4 results). Not uploaded for now.

Pick up LTAR field samples from Josh, dump LTER leftover plants - Jon

GC Sequence BCSE20260205S25BGC vials submitted out of sequence by techs. All vials run in numerical sequence on GC. Vials submitted out of sequence as follows: …31,32,34,33,35,36… …50,51,56,53,54,55,52,57,58….

Lachat run LTER soil 20250806.

GC Sequence LTER20260206S18BGC notes. Vials 13-16 were not sampled (techs couldn’t find chamber in the snow), but run on GC as empty vials to keep run in order. Tech notes that vials 73-112 contained swapped sets (i.e. vial 93-96 were used for sheet slots 73-76). These were corrected on the field note sheets and entered at the field note data entry to properly line up the vials with the sites

Grind BCSE plants - Jon

Pack LTER plant 2023 CN - Brian

Measured the dimensions of the various towers LxWxH KK, DW Set 1 (Vizza Roley) 14 x 16 Single riser 64”. New wheels ground to top of riser is approx. 80” Qty 4 This is the only set with a solid riser.

Set 2 (Vizza/Roley) 14x16’ 2’ +3’ riser ground to top approx same as above if we change the wheels to new type. Qty 4

Set 3 12’ x 12’ joint to top of riser is approx 36-40” (snow). Riser ground to top is 57ish” Qty 4

Set 4 old 14x16’ ground to riser top is 52” If set 3 and 4 had same wheel type height would be equiv. Both use 3’ riser.

Set 5 ALL REX Shelters 14x18’

LTAR tower 1 had irgason errors. Checked around 2:30PM and found bird droppings on the lower lens. Cleaned with a KIM Wipe. Now reading fine.

Checked other sensors. all look good.

Prepared Standards, Lachat run LTAR soil 20250908.

Grind BCSE plants - Brian

Pack LTER plant 2023 CN - Jon

Grind ASP6 POACEAE (combined grasses) on campus, deliver BCSE grain harvest samples to Chris - Jon, Brian

Grind BCSE plants - Olivia

Acid washed dishes, reconditioned cadmium column.

Lachat run LTAR soil 20250813.

Grind BCSE plants - Sarah

Pack CN - Brian

Sort ASP6 samples to grind on campus 2/10/2026 - Jon

LTAR Series 32 gas sampling - Brian, Jon, Sarah

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Lachat run LTER T4 T21 Soil 20250723.

• Samples T4R2S4, T4R4S1, and T4R4 strip were not processed, not included.

LTER Series 18 gas sampling - Alyssa (am), Jon, Brian, Olivia (pm)

Check LTAR gas chambers ahead of 2/9/2026 sampling -