Noticed an issue with inconsistent check standards on LTAR202504100417S4S5BGC run. Some checks made before, some after, a nitrogen tank change-out. (N2 used in making up standards.) High standards, with no N2 addition, appear to be fairly consistent across the last 3 sample runs. A separate standard/check std run was completed after the LTAR run to test for this issue. This standard run would likely be better to use with the LTAR run. Standard run was STD_TEST20250424BGC.

Prepared 1M KCL.

LTAR202504100417S4S5BGC sample run on GC had vials submitted out of order on trays. All samples were run on GC in numerical order. Samples submitted out of order as follows: …4, 5, 9, 7, 8, 6, 10, 11, 12… and …135, 136, 140, 137, 138, 139, 141, 142….

BCSE202503310416S2S3BGC sample run on GC had a small set of samples submitted out of order on the trays. All vials were run on the GC in numerical order. Vials out of order on trays as follows: ….56, 57, 59, 58, 60, 61, 62, 63…..

Prepared Standards, Replaced Cadmium Column.

Lachat run LTER Lysimeters 20240617.

Reconditioned Cadmium Column. Lachat run LTER soil 20211112.

Acid washed dishes.

Dishes, grind SBE plants, pulverize BCSE deep core soil -

LTAR 5 gas sampling -

LTAR Hydrosense - Alyssa

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Replaced Reagent tubing, finished Lachat run GLBRC Microplot soil 20241111.

Collect LTAR leachate - Andie, Trajana

BCSE 3 gas sampling - Slater, Brian

BCSE Hydrosense - Alyssa

Measure, filter and archive LTAR leachate; one spring sampling and one fall sampling will be archived each year, 20mL sample with conical seal cap (same as LTER) -

Filter LTAR soil extracts -

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run GLBRC Microplot soil 20241111.

Prepared Standards, Lachat run GLBRC Main soil 20241104.

Evacuate LTAR lysimeters, prep BCSE and LTAR gas chambers - Olivia, Brian

Weigh LTAR soil for extraction and moisture - Slater, Trajana

Grind SBE plants and pulverize BCSE deep core soil - Olivia, Brian

Prep BCSE and LTAR gas vials and totes - Slater, Trajana

LTAR April soil sampling, first sampling to include new R5 plots - Alyssa, Slater, Brian, Trajana

Sieve LTAR April soil - Slater, Brian, Trajana, Andie

Grind plants, pulverize BCSE deep core soil, weigh dry soil tins, dishes - Slater, Brian, Trajana, Andie

GC run LTAR202503260403S2S3BGC had several vials mixed up in one series 3 tray received from the techs. All vials run in sequential order on the GC. Here is the order presented in the trays for series 3: …23, 24, 25, 46, 47, 48, 29, 42, 43, 44, 33, 38, 39, 40, 37, 34, 35, 36, 41, 30, 31, 32, 45, 26, 27, 28, 49, 50, 51, 52, etc….

LTER Gas Series 3 - All + hydrosense (Alyssa, Andie) (Forest set DF2, CF2, SF3 split and sampled by two people due to issue with golf cart)

Dishes - Brian

Added CS650 sensors to T7r3,4,2,6 (did T7 r1,5 2 weeks ago). Added positions to Survey 123 and hard copy map.

All chambers and bases are removed from BCSE G1 and 5. Compressor shut down in December for last measurements. Keeping both analyzers running to keep stable. Waiting for electric at LTAR R5 to move system.

Finish LTER Lysimeter filtering - Alyssa

Prep LTER gas totes, vials - Brian

BAF subsampling - Slater, Finish grinding GLBRC plants, mix air drying soil - Trajana

LTAR Gas Series 4 - Trajana, Slater, Brian + Hydrosense (Alyssa)

Finish LTER gas frame prep - Alyssa

Acid washed dishes.

Collect LTER Lysimeters - Slater, Andie

Prep LTAR, LTER gas frames - Brian, Trajana

Recondition Cadmium Column. Lachat run GLBRC MLE MS soil (Lux Arbor) 20241106.

Filter LTER soil extracts - Brian, Slater

Measure and filter LTER lysimeter leachate - Andie, Trajana

Prepared 1M KCL Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER soil 20241023.

Evacuate LTER Lysimeters - Brian, Trajana

Finish sieving LTER soil - Slater

Weigh LTER soil for extraction and moisture - Alyssa, Olivia, Slater

LTAR vial, tote prep - Olivia. BAF subsampling - Slater

LTER Soil sampling - Alyssa, Brian, Slater, Trajana

Dishes, Grinding Plants - Slater, Olivia

Prepared Standards, Lachat run GLBRC MLE WC soil (Hancock) 20241017

LTAR Series 3 gas sampling - Slater, Andie, Alyssa (+Hydrosense), Olivia

Collect LTAR leachate from 4/1 evacuation - Slater, Andie, Alyssa, Olivia

Collection of LTAR leachate had to be delayed until 4/3 due to all day heavy rain and storms.

Grind plants and finish SWF deep core archiving and subsampling - Slater, Andie

LTAR gas vial and tote prep - Stacey

Grind plants, pulverize BCSE deep core soil, SWF deep core archiving and subsampling, field lab clean-up - Olivia, Slater, Andie

Evacuate LTAR lysimeters; LTAR gas chamber prep - Slater, Andie

BCSE Series 2 gas sampling - Slater, Andie (Hydrosense too)

SWF deep core archiving and subsampling - Slater, Andie

Grind plants; pulverize BCSE DC soil; weigh, archive and subsample SWF DC soil - Slater, Trajana, Andie, Olivia

Dishes, BCSE gas vial prep, weigh dry soil tins - Brian

BCSE gas chamber prep -

LTER 2 gas sampling (first full set for 2025), prairie strips had to be sampled in the afternoon - Slater, Brian, Trajana, Olivia

LTER Hydrosense; Pull LTAR ASP1, ASP5, ASP6 frames for fertilizer and mowing - Alyssa

Filter LTER soil extracts, prep LTER gas vials/totes, dishes - Slater, Brian, Trajana

Sieve LTAR R5 soil, remove subsample to send to A&L for S1 package, set the rest out to air dry for archive - Stacey

LTAR 2 gas sampling - Slater, Brian, Trajana

LTAR Hydrosense - Alyssa

Sampled soil from new LTAR R5 plots, twelve 0-25cm cores per plot pooled, six from the north half and six from the south half, approx. evenly space across the plot EW; when a row crop was present alternated taking a core in the row and between rows - Stacey

Lachat run LTER T4 Soil 10/22/2024.

Prepared 1M KCL, Reconditioned Cadmium Column.

Lachat run LTER T3 soil 10/21/2024.

Finish sieving LTER soil - Slater, Trajana

Weigh LTER soil for extraction and moisture - Brian, Olivia

Prep LTAR gas vials/totes, dishes - Brian, Olivia

Prep LTAR and LTER gas chambers, install T7 and T7 microplot chambers - Slater, Trajana

First 2025 LTER soil sampling, two 0-25cm cores NE of each station, did not sample T6 since flags had been pulled and switchgrass is still not harvested - Alyssa, Slater, Brian, Trajana, Andie

Sieved LTER soil - Alyssa, Slater, Brian, Trajana, Andie

Weigh and grind plants, pulverize BCSE deep core soil - Slater, Trajana, Brian, Olivia

Lachat run LTAR soil 9/30/2024.

Replace T7 station flags, fix all other LTER, BCSE, LTAR station flags that were leaning or fallen -

Acid washed dishes.

Finish filtering LTAR leachate, dishes - Brian

Weigh and grind plants, archive air-dried soil, pulverize BCSE deep core soil - Slater, Trajana, Brian, Olivia

Lachat run GLBRC MLE WN soil (Rhinelander) 10/16/2024.

Collect LTAR leachate - Brian, Trajana

GC run BCSE20250307S1BGC had one series of vials submitted out of numerical order. All vials run on the GC in numerical order, but submitted series were …60, 62, 61, 63, 64, 65, etc… (so just 61 and 62 submitted out of order, but subsequently run in numerical order).

Filter LTAR soil extracts - Brian, Trajana

Weigh LTAR soil for moisture and N-extraction -

Weigh and grind plants, pulverize BCSE deep core soil, subsample/archive SWF deep core soil - Slater, Trajana, Brian, Olivia

Prepared standards, prepared 1M KCL.

Lachat run CLE inorganic N (Hammond) June 2024 soil.

Evacuate LTAR lysimeters -

GC run LTER20250306S1BGC: samples 241 and 242 were switched out of order in the trays submitted by the techs (likely just an oversight). Vials all run in numerical order on GC.

First LTAR soil sampling for 2025, two 0-25cm cores per station (NE of station) - Alyssa, Slater, Trajana, Brian

Weigh ASP6 biomass sampled on 3/12/2025 - Stacey

Sieve LTAR soil - Alyssa, Slater, Trajana, Brian

Weigh and grind plants, pulverize 2022 BCSE deep core soil - Slater, Trajana, Brian

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Weigh and grind plants, pulverize 2022 BCSE deep core soil (Alyssa trained), subsample and archive SWF deep cores soil - Slater, Brian, Andie, Trajana, Olivia

LTAR Series 1 gas sampling - Slater, Brian, Olivia

LTAR Hydrosense - Alyssa

Weigh and grind plants - Brian, Olivia

Subsample and archive SWF DC soil - Slater

Lachat run LTAR soil 8/28/2024.

Reconditioned Cadmium Column.

Lachat run LTER Soil 9/17/2024.

Finish filtering LTER leachate, prep LTAR gas vials and totes - Alyssa

Weigh and grind plants - Andie, Brian

Subsample and archive SWF DC soil - Trajana, Slater

LTAR gas chamber prep, pull ASP4 microplot chambers and install in ASP3 microplots, sample ASP6 biomass to approximate what could be harvested… from each station, 1.5mN, 0mW to SE corner of 2 x 0.5m frame, plants cut at 6 inches above the ground with electric trimmers, bagged as UNSRT, if plants were lodged or lying down - they were not stood up for cutting. - Slater, Brian, Andie, Trajana

Measure and filter LTER leachate - Brian, Trajana, Olivia

Weigh SWF deep core moisture samples - Slater

Collect LTER leachate - Slater, Brian

Pull T7 flags and gas frames for burning - Olivia, Trajana

Burn all T7 plots - Kevin, Sven, Dave, Stacey, Alyssa, Olivia, Trajana, Brian, Slater, plus several others (about 16 total)

Evacuate LTER lysimeters (first for 2025) - Slater, Trajana, Brian, Andie

Weigh and grind plants - Trajana, Slater

Weigh SWF deep core moisture samples - Andie, Brian

Acid washed dishes.

Lachat run LTAR soil 7/23/2024

Finish processing SWF deep cores - Slater, Olivia

Weigh and grind plants - Trajana, Brian

BCSE Series 1 gas sampling - Brian, Slater

Hydrosense in LTER and BCSE - Kevin trained Alyssa (MCSE and BCSE) and Stacey (DF)

Evacuated LTER lysimeters that were installed in fall 2024 since conditions were very dry many did not yield leachate in November or December 2024. These lysimeters were evacuated on 3/7/2025 and left over the weekend. The lids were completely off from DF3-1 and DF3-6. On 3/11/2025 they were checked for pressure, the volume of leachate approximated and then the leachate dumped before re-evacuating with the regular sampling. - Stacey

Lysimeter, pressure, volume from 3/11/2025; CF1-1, N, 0; CF1-3, N, 0; CF1-6, N, 0; CF2-1, N, 20; CF2-3, Y, 300; CF2-6, Y, 200; SF3-1, Y, 600; SF3-3, Y, 450; SF3-6, Y, 200; DF3-1, Y, 350; DF3-3, Y, not recorded; DF3-6, N, not recorded; CF3-1, Y, 50; CF3-3, N, 50; CF3-6, Y, 200

Prepared Standards, Acid washed dishes.

Lachat runs LTAR soil 5/28/2024 and 6/24/2024.

Process SWF deep cores - Brian

LTER Series 1 gas sampling (last winter sampling) - Slater, Olivia, Trajana

Weigh and grind plants - Trajana, Slater, Brian, Andie

Process SWF deep cores - Andie, Olivia, Slater

Lachat run LTER soil 8/14/2024.

Collect LTAR leachate - Slater, Olivia, Brian, Trajana

Measure and filter LTAR leachate, dishes - Slater, Olivia, Trajana

Process SWF deep cores - Brian

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Evacuate LTAR lysimeters (first for 2025), ASP6R4/ASP2R4 box was frozen shut and couldn’t be opened, so those lysimeters were evacuated on 3/4/2025 and collected 3/5/2025. - Slater, Brian, Andie, Trajana

Weigh and grind plants - Slater, Brian

Process SWF deep cores - Trajana

Grind BCSE plants on campus - Andie, Trajana

Dump 2024 LTER leftover plants and 2023 soil samples - Brian, Slater

Reconditioned Cadmium Column, Acid washed all Channel 2 reagent containers.

Lachat run LTER soil T4 T21 7/8/2024.

Weigh and grind plants - Slater, Trajana

Process SWF deep soil cores - Brian

Replaced weather station battery at Hancock. Tagged instrumetations and some sensors.

Almost no snow.

Acid washed dishes, Lachat run LTER soil T3 T21 7/8/2024.

Replaced the weather station batteris at Escanaba and Rhinelander. Tagged instruments and some sensors with barcodes.

Minimal snow at either location.

Weigh and grind plants - Trajana, Brian

Process SWF deep soil cores - Andie, Slater