Wires trenched in at both new LTAR new towers. Waiting for sensors.

ASP Tower 2 and Bau tower 2 both infrastructures installed over the course of the week. Tower positions measured by Brook Wilke, with Kevin present. Sensors will come from Jiquan Chen.

Uploaded GLBRC Scale up Lux Arbor and Marshall samples in multiple data sets due to partial contamination. Notes are as follows:

L1 and L3 samples from the Lux Arbor Scale up field (12/2/2024) had Ammonia contamination in KCL used, so all ammonia results were discounted. L2 was sampled on different date (12/16/2024) and had no contamination, results will be uploaded in a separate file.

M1, M2 and M4 samples from the Marshall Scale up field (12/2/2024) had Ammonia contamination in KCL used, so all ammonia results were discounted. M3 was sampled on different date (12/16/2024) and had no contamination, results will be uploaded in a separate file.

Dropped BAU Tower 1 to standard 2m height after corn harvest. Will take some time to move ground sensors back as 8/9 destroyed by coyotes. Tower height was very easy to drop down.

BAU Tower 79-2 ground sensors removed for harvest this morning. Found all sensors destroyed even after we covered them with corrugated cover. Coyote went right to the sensor and chewed off in all cases.

ASP3 corn sampling (same as BAU2): R1-4 all stations: 0.66mS, 0.66mW to NE corner of 1.52 x 0.66m frame, oriented with the 0.66m ends on the east and west. Perpendicular to corn rows. R5: Place the frame approximately in the center of each half of the plot (east and west halves), with the 0.66m frame ends oriented towards the north and south. - Slater, Brain, Olivia, Sarah, Emily, Ainsley

Innovations Plot 1 (IP1) Short Corn Trial sampling: Area is split into East and West halves. Each half includes the same three treatments: Short Corn, Control 1 and Control 2. Six samples will be taken from each treatment, three from the east (labeled south, center, north) and three from the west (labeled south, center, north). The IP1 field is 85m from south to north, same as ACSE plots. So, the same distances from the south end were used to match the station flag distances from the south end: south was about 12m from the south end, center about 45 m from the south end, and north about 64m from the south end. Per Brook’s instructions on the EW sampling locations, short corn was sampled near the center of the eastern half of each “rep”, otherwise when planted in 3 rows - rows 2 and 3 from the west were sampled and when planted in 6 rows - rows 5 and 6 from the west were sampled. Use 1.52 x 0.66m frame, oriented with the 0.66m ends on the east and west. Perpendicular to corn rows.- Slater, Brain, Olivia, Sarah, Emily, Ainsley, Alyssa

LTER Series 14 gas sampling - Brian, Jon, Ainsley, Slater, Emily

LTER Hydrosense - Alyssa

Filter LTAR soil extracts, dishes - Slater, Emily

Pack CN - Stacey

Weigh LTAR soil for extraction and moisture - Brian, Ainsley

LTER gas vial and tote prep - Slater

Transect data entry - Jon

Pack CN - Stacey

LTER gas chamber prep -

Collect leaf litter from traps in T5, G8, CF, DF, SF -

LTAR October soil sampling -

Thresh soybeans -

Transect data entry - Brian, Ainsley

Weigh plants - Sarah

Pack CN - Stacey

Pack CN - Stacey

Weigh plants - Brian, Jon

Plant species composition transect data entry - Emily, Slater, Ainsley, Brian

Weigh dry soil tins, dishes - Jon

Shred G3 sorghum - Alyssa, Ainsley, Ethan, Jarrod

Finish G6 ANPP - Alyssa, Ainsley, Brian, Jon

Install LTAR station flags - Slater, Emily

REX logger T7R4 (197) did not have a running program. When connecting through the loggernet network, only the status table was visible and the logger did not accept a new program sent over the network. When connecting directly to the logger over usb, it showed all of the programs intact, but none of them running. I marked the REX program as the one to run right away and on power up. I’m not sure how the logger got into this state.

Continue G6 ANPP sampling - Slater, Jon, Emily, Alyssa, Brian, Sarah, Ainsley

Plant species composition transect data entry 2025 - Olivia, Brian

Dishes - Sarah

Weigh plants - Ainsley, Alyssa

Thresh wheat

Finish BAU2 corn sampling. Start BCSE G6 species composition: Three NS transects per plot, main only. Each transect ~2m east of the station flags and numbered 1-3 based on the station number; and ANPP sampling: Use 1.52 m x 0.66 m frame. Station 1 only: 1.5mN, 0mW to SE corner of 1.52x0.66m frame, to avoid microplot. Station 2, 3: 1.5mN, 1.5mW to SE corner of 1.52x0.66m frame.

Transect data entry - Ainsley

Transect data entry - Alyssa

Measure and filter LTAR leachate - Brian, Emily

Pack CN (LTER 2020 plants) - Stacey

Collect LTAR leachate - Brian, Emily

LTAR Series 25 gas sampling - Jon, Ainsley, Slater, Alyssa (Hydrosense)

Thresh wheat - Brian, Ainsley

Sample BAU2 corn: R1-4 all stations: 0.66mS, 0.66mW to NE corner of 1.52 x 0.66m frame, oriented with the 0.66m ends on the east and west. Perpendicular to corn rows. R5: Place the frame approximately in the center of each half of the plot (east and west halves), with the 0.66m frame ends oriented towards the north and south. - Alyssa, Slater, Jon, Emily

Transect data entry - Olivia, Ainsley, Brian, Jon

LTAR gas vial and tote prep - Brian, Sarah

Filter LTER T3, T4 soil extracts - Slater, Sarah, Emily

Pack CN (LTER 2020 plants) - Stacey

Evacuate LTAR lysimeters - Slater, Alyssa

Thresh wheat - Emily, Jon, Alyssa, Ainsley

LTAR gas chamber prep, pull G2 flags and frames for soybean harvest - Brian, Sarah

Collect LTER leachate - Alyssa, Emily

Thresh LTER wheat - Slater, Emily

Weigh T3, T4 soil for extraction and moisture - Brian, Ainsley, Jon

Measure and filter LTER leachate, dishes - Alyssa, Emily, Jon

Plant species composition transect data entry - Slater, Ainsley, Brian

LTER T3, T4 soil sampling, two cores per station (one in former wheat row, one from between rows), four cores per prairie strip spread across strip (avoiding edges, foot paths, gas frames and no-burn microplot) -

Evacuate LTER lysimeters -

Thresh LTER wheat -

Sieve LTER T3, T4 soil samples -

Plant species composition transect data entry -

Collect leaf litter from traps in T5, G8, CF, DF, and SF -

Thresh LTER wheat -

Finish G3 sorghum ANPP sampling - Alyssa, Emily, Ainsley, Slater, Jon

Plant species composition transect data entry - Brian

All LTAR 2024 Soil and Lysimeter Lachat runs uploaded.

BCSE Series 20 gas sampling -

BCSE Hydrosense - Alyssa

Thresh LTER wheat -

Continue BCSE G3 ANPP sampling -

GC Sequence LTAR20250918S24BGC had vials submitted out of order. All vials run in sequential order, but these two vials were out of order as submitted: 34 and 33.

BCSE gas chamber prep - Ainsley

Thresh LTER wheat - Jon, Slater, Sarah

Finish clipping G1 ANPP. Start sampling G3 sorghum ANPP: Use 1.52 m x 0.66 m frame, All stations except G3R1S1: 1.5mN, 1.5mW to SE corner of 1.52x0.66m frame, Only G3R1S1: 1.5mN, 0mW to SE corner of 1.52x0.66m frame, to avoid microplot. Weigh wet prior to putting into the oven. - Jon, Brian, Emily Ainsley

BCSE gas vial, tote prep - Stacey

Finish sorting SF1 ANPP - Olivia, Sarah, Emily

Weigh LTAR wheat - Brian, Stacey

LTER Resource Gradient soil sampling. Four 0-25cm cores per plot, two in the row of wheat stubble and two from between rows. Spread across the plot, avoiding at least 1m around the edges. - Alyssa, Ainsley, Emily, Jon

Sieve and package LTER Resource Gradient soil to send to A&L. - Alyssa, Ainsley, Emily, Jon

Weigh LTER wheat - Slater

WPS training - Brian

Lab clean-up, dishes - Brian

Plant species composition transect data entry - Ainsley

Weigh LTER wheat - Brian

Sort SF1 ANPP samples -

Finish BAU1 soybean sampling. Start BCSE G1 corn ANPP sampling. Station 1 only: 1.5mN, 0mW to SE corner of 1.52x0.66m frame, to avoid microplot. Station 2, 3: 1.5mN, 1.5mW to SE corner of 1.52x0.66m frame. -

GC sequence LTAR202508210903S22S23BGC had a few vials submitted out of order. All samples run in numerical sequence on GC. These vials were out of order for Series 22: …112, 115, 113, 114, 116, 117…

GC sequence LTER20250819S12BGC had some vial order issues as noted from techs. All vials run in sequential order on GC. Note from techs that vials 185 and 186 were swapped and that vials 145-153 were swapped with vials 173-181, as noted on the data sheet.

Finish sorting T6 ANPP - Stacey, Alyssa, Jon

Finish GLBRC Scale-up plant species composition transects, Marshall - Alyssa, Jon, Slater, Emily

Continue sampling ASP4, BAU1 soybeans - Brian, Trajana, Ainsley, Olivia, Slater, Emily

GC Sequence LTER20250911S13BGC had a few vials submitted out of order. All vials were run in sequential order on the GC. Vials submitted out of order as follows: …201, 202, 204, 203, 205, 206… and then …238, 239, 252, 241, 242, 243, 248, 245, 246, 247, 244, 249, 250, 251, 240, 253, 254, etc.

Sort T6 ANPP samples - Trajana

Sort SF2, SF3 ANPP samples - Stacey

LTAR Series 24 gas sampling, hydrosense - Alyssa, Slater, Jon, Trajana

Finish BCSE G2 soybean sampling. Start ASP4, BAU1 soybean sampling. R1-4 all stations: 0.5mS, 0.5mW to NE corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. Perpendicular to soybean rows. R5: Place the frame approximately in the center of each half of the plot (east and west halves), with the 0.5m frame ends oriented towards the north and south. The frame should be perpendicular to soybean rows. Be sure rows are centered in the frame such that the space at each end beyond the outside rows is equal. - Emily, Ainsley, Slater, Trajana

GLBRC Scale-up plant species composition transects, Lux - Alyssa, Jon, Emily, Ainsley

LTAR gas chamber prep - Ainsley

Clip SF ANPP. 2mN, 4mW of each station flag to SE corner of 2 x 0.5m frame - Brian, Emily, Sarah, Trajana, Olivia, Jon, Slater

Clip BCSE G2 soybean ANPP samples. All stations except G2R1S1: 1.5mN, 1.5mW to SE corner of 1.52x0.66m frame. Only G2R1S1: 1.5mN, 0mW to SE corner of 1.52x0.66m frame, to avoid microplot. - Brian, Emily, Sarah, Slater

Prepared Standards, Acid washed dishes.

Lachat run LTAR N-Inhibitor soil 20250807 (No blanks were made for this set, so unable to determine potential contamination).

Weigh LTER wheat - Slater, Trajana

LTAR gas vial, tote prep - Ainsley

Filter LTER soil extracts - Jon, Ainsley

Collect LTAR leachate - Jon, Emily

Finish clipping T6 ANPP samples - Brian, Slater, Trajana, Ainsley

Weigh LTER soil for extraction and moisture - Slater, Ainsley

Measure and filter leachate, dishes - Jon, Emily

Weigh LTER wheat samples - Trajana

Sort T6 ANPP - Brian

Acid washed all Channel 2 reagent containers, dishes.

Prepared 1M KCL.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

LTER September soil sampling, used bucket augers to sample T5, 7 only -

Evacuate LTAR lysimeters -

Continue clipping T6 ANPP samples -

Sieved LTER soil samples -

Transect data entry - Trajana, Ainsley, Stacey

Sort T6 plants - Alyssa, Jon, Ainsley

BCSE Series 19 gas sampling - Alyssa, Slater, Trajana - Hydrosense

Collect T5, G8 LFLIT - Olivia, Jon

Set forest leaf litter traps - Brian, Emily, Slater, Trajana

LTER Series 13 gas sampling - Slater, Jon, Emily, Brian, Ainsley

LTER Hydrosense and finish GLBRC MLE MS plant composition transects - Alyssa, Trajana

Continue T6 ANPP sampling - Brian, Ainsley, Trajana, Alyssa

BCSE gas chamber prep - Stacey

BCSE gas vial and tote prep, sort T6R1S2,3 plants to evaluate switch from scissors to electric trimmers = small plants still in good shape, no significant difference in quality of cut samples, will use electric trimmers going forward. - Stacey

LTER gas vial and tote prep, transect data entry - Alyssa

Sort T6 plants - Stacey, Trajana

Filter LTAR soil extracts - Ainsley, Sarah

GLBRC MLE MS plant composition transects - Alyssa, Jon, Emily, Brian

LTER gas chamber prep -

T6 ANPP sampling -

Collect LTER leachate - Brian, Ainsley

Start T6 ANPP sampling, 2mS, 4mW to NE corner of 2 x 0.5m frame. - Emily, Slater, Trajana

GLBRC MLE MS plant composition transects - Alyssa, Slater, Emily, Trajana

Weigh LTAR soil for extraction and moisture - Alyssa, Jon

Filter LTER leachate, dishes - Brian, Ainsley

Sort plants - Jon

Prepared 1M KCL

Sort T7 plants - Alyssa, Ainsley, Stacey, Slater, Emily, Brian

Dishes - Jon, Trajana

Grind 2024 LTAR fields plant samples - Sarah

LTAR September soil sampling - Alyssa, Ainsley, Jon, Trajana, Olivia

Evacuate LTER lysimeters - Slater, Sarah

Replace LTAR ASP2 flags - Emily, Brian

Sort T7 plants - Stacey, Ainsley, Emily, Trajana

GLBRC MLE MC plant composition transects - Alyssa, Slater, Brian, Jon

Finish BCSE G4,5,7,9,10 ANPP sampling - Trajana, Olivia, Ainsley, Emily

Sort T7 plants - Emily, Ainsley

GLBRC MLE MN plant composition transects - Alyssa, Slater, Brian, Jon

LTAR field day, help with parking - Ainsley, Emily, Trajana

LTAR Series 23 gas sampling - Emily, Ainsley, Olivia, Trajana - Hydrosense

Finish clipping T7 ANPP with R6 S4,5 - Sarah, Trajana

MLE WN plant species composition transects - Alyssa, Slater, Brian, Jon

GC run sequence Blesh-UDF20250825S26.BGC had the following issues:

1. vials all run in sequence, but 2 vials submitted in rack out of order - vial 55 and 56 were reversed in position when received from field
2. Visual computer signal lost on run startup, but data seemed to be collecting fine. Leter found data stopped being collected at about sample/std vial 19 or so. Rebooted computer to restart run the next morning.
3. Following morning discovered the Licor box detector was shot, throwing wild erroneous peaks and valleys. Box was replaced with AnnaGC box. Restarted sequence near where it left off, with a new “B” sequence.
4. No peaks were seen on “B” sequence. Stopped and restarted. Autosampler proceeded to bend the injection needle like an accordion, before even attempting to draw sample. So, shut everything down to investigate.
5. Discovered nitrogen port on syringe had been sheared off. Replaced needle and syringe and rebooted everything - GC, Autosampler, and Computer.
6. Deleted “B” run due to null data and restarted “B” run all over again. Everything seems to be working now…

Sort T7 plants - Trajana, Sarah, Ainsley

Clip T7 ANPP R4, 5 all, R6 S1-3 - Ainsley, Emily

LTAR gas chamber prep - Stacey

MLE WC plant species composition transects - Alyssa, Slater, Brian, Jon

Collect LFLIT from T5 and G8, then trim under traps. Continue BCSE G4,5,7,9,10 ANPP clipping. - Ainsley, Emily, Olivia

Sort T7 ANPP - Ainsley, Emily, Stacey, Slater, Jon, Trajana

BCSE Series 18 gas sampling - Slater, Jon, Trajana (Hydrosense)

Continue clipping BCSE G4,5,7,9,10 ANPP - Ainsley, Emily, Slater, Jon, Trajana

Sort T7 ANPP -

BCSE gas vial, tote prep - Ainsley

Continue clipping BCSE G4,5,7,9,10 ANPP -

BCSE gas chamber prep - Ainsley

Finish BCSE G4,5,7,9,10 transects and continue clipping ANPP - Alyssa, Brian, Jon, Olivia, Emily, Ainsley, Slater, Trajana

Sort T7 ANPP - Stacey

Sort T7 ANPP - Stacey, Olivia, Sarah, Trajana

BCSE G4,5,7,9,10 species composition transects: Three NS transects per plot, main only. Each transect ~2m east of the station flags and numbered 1-3 based on the station number. Stop about every 1m to identify and measure first intercepted plant. - Alyssa, Brian, Jon, Emily, Slater

Clip T7 R1-3 ANPP: 2mS, 2mE to NW corner of 2 x 0.5m frame. Clipped with scissors, refrigerated and sorted to species in the field lab. - Olivia, Trajana, Ainsley, Sarah

Start clipping BCSE G4,5,7,9,10 ANPP: Stations 2, 3: 2mN, 2mW to SE corner of 2 x 0.5m frame, Station 1 only: 2mN, 0mW to SE corner of 2 x 0.5m frame, to avoid microplot. Clipped with electric trimmers and left as UNSRT. - Ainsley, Emily

Sort ASP5 plants -

Measure and filter LTAR leachate - Ainsley, Slater

Filter LTER soil extracts -

Collect LTAR leachate -

Replace LTER station flags -

LTAR Series 22 gas sampling - Olivia, Emily, Jon, Trajana (Hydrosense)

Evacuate LTAR lysimeters - Ainsley, Slater

Prepared 1M KCL

Weigh LTER soil for moisture and N-extraction - Sarah, Brian

Sort ASP2 plants - Alyssa, Olivia, Emily, Jon, Trajana, Ainsley, Slater, Brian

Dishes - Sarah

The T7_R4 logger has not been responding since the 19th of August. I had briefly connected to the logger over USB using device manager, just to test if I had remembered the security code. I did not interact with the logger otherwise. Today the logger activity light was on solid. I power cycled and was able to connect, but the logger seemed to be reformatted. Only the system table was there. I reloaded the program and restarted data collection, but the data between the 19th and 21st are lost.

LTER soil sampling - Alyssa, Ainsley, Sarah, Slater, Emily, Olivia

LTAR gas chamber prep - Trajana

Sticky traps - Brian, Jon

Finish clipping ASP2 - Stacey

Clip ASP5 ahead of herbicide. R1-4 all stations: 1mS, 0.5mW to NE corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. R5: Place the frame approximately in the center of each half of the plot (east and west halves). The frame should be perpendicular to crop rows. Sorted in the field lab later to Red clover, Trifolium pratense, TRFPR; Berseem clover, Trifolium alexandrinum, TRFAL; TRIAE (wheat) and remainder label UNSRT in the field lab. - Slater, Olivia, Emily, Trajana

LTAR202507170724S18S19BGC sequence had a vial order issue. There were vials submitted out of order for Series 18 such that I ran them in sequence 31, 32, 36, 33, 34, 35, 37, 39, 38, 40, 41 for Round “A,” but then forgot to make the same change for Round “B.” So Round B was entered in the sequence as running in consecutive order (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, etc.). Please refer to data sheets for clarification on this. I will change the Round “B” labels in the Excel results file to properly match Round “A.”

Sieve LTER soil - Alyssa, Ainsley, Sarah

LTAR gas vial and tote prep - Trajana

LTER Series 12 gas sampling - Alyssa, Sarah, Jon, Ainsley, Emily, Olivia

LTER Hydrosense - Trajana

Final prairie strip sticky traps, pull T-posts - Slater, Jon, Brian

Clip ASP2 forage ahead of harvest, R1-4 stations 1, 2, 3: 1.5mS, 0.5mW to NE corner of 2 x 0.5m frame, oriented with the 0.5m ends on the east and west. R5: Place the frame approximately in the center of each half of the plot (east and west halves). Oriented perpendicular to forage rows. Sorted in the field lab later: MEDSA, Medicago sativia, alfalfa; TRFPR, Trifolium pratense, red clover; CICSP, Cichorium spp, six-point chicory; DACGL, Dactylis glomerata, orchard grass; BRSNA, live volunteer canola; and remainder UNSRT. - Slater, Emily, Olivia

Filter soil extracts, dishes - Brian, Ainsley, Sarah

Sort plants - Stacey

LTAR202507310807S20S21BGC sequence had vials submitted out of order. All vials run on GC in consecutive order. Vials out order as follows: …125, 126, 131, 132, 129, 130, 127, 128, 133, 134…. Also “Dup” vials were all submitted upside down in sampling rack. Could mean they weren’t sampled?

LTER gas chamber prep - Brian, Slater, Trajana

Finish clipping T3, T4 prairie strip ANPP - Emily, Slater, Jon, Sarah

Weigh T21 incubated soil from T3, T4 stations and prairie strips - Sarah, Ainsley, Emily

Dishes - Jon, Ainsley

LTER gas vial and tote prep - Olivia

Sort plants - Alyssa, Stacey, Olivia, Trajana

Grafana seems to only have updated data through August 4th. Would be helpful to see if any sensor changes made in the last week were live or still defunct. Today - T7R4 fixed splice on sensor “k” and reconnected. It is our sensor furthest away from the box. Replaced sensor “i” with a different sensor “i” we had in inventory - the original had 2-3 cuts deep enough to break the signal. T7R5 - added a “new” sensor “8” back into the location the previous sensor 8 was removed from. Discovered sensor “9” had a disconnected green line. Changes were made in Survey123 app except for sensor “k” at T7R4 plot.

BCSE2025072507310814S15S16S17BGC GC sequence had vials submitted out of order. All vials run on GC in consecutive order. Vials that were out of order were as follows: S16 - …16, 17, 19, 18, 20, 21… and vial #75 and vial #Dup3 were switched for each other; S17 vials in order except as noted here - …60, 61, 78, 79, 80, 65, 74, 75, 76, 69, 70, 71, 72, 73, 66, 67, 68, 77, 62, 63, 64.

Collect LTER leachate - Jon, Sam

Collect T21 soil incubation bags from T3, T4 stations and prairie strips - Sarah, Emily, Olivia, Trajana

Collect T5, G8 LFLIT and trim under traps - Brian, Ainsley, Slater

Sort plants -

Filter leachate, filter LTAR soil extracts, dishes -

Weigh LTAR soil for extraction and moisture - Brian, Ainsley

Sort plants - Brian, Emily, Ainsley, Sam, Trajana, Slater, Jon