Grind plants; pulverize BCSE DC soil; weigh, archive and subsample SWF DC soil - Slater, Trajana, Andie, Olivia

Dishes, BCSE gas vial prep, weigh dry soil tins - Brian

BCSE gas chamber prep -

LTER 2 gas sampling (first full set for 2025), prairie strips had to be sampled in the afternoon - Slater, Brian, Trajana, Olivia

LTER Hydrosense; Pull LTAR ASP1, ASP5, ASP6 frames for fertilizer and mowing - Alyssa

LTAR 2 gas sampling - Slater, Brian, Trajana

LTAR Hydrosense - Alyssa

Sampled soil from new LTAR R5 plots, twelve 0-25cm cores per plot pooled, six from the north half and six from the south half, approx. evenly space across the plot EW; when a row crop was present alternated taking a core in the row and between rows - Stacey

Lachat run LTER T4 Soil 10/22/2024.

Filter LTER soil extracts, prep LTER gas vials/totes, dishes - Slater, Brian, Trajana

Sieve LTAR R5 soil, remove subsample to send to A&L for S1 package, set the rest out to air dry for archive - Stacey

Finish sieving LTER soil - Slater, Trajana

Weigh LTER soil for extraction and moisture - Brian, Olivia

Prep LTAR gas vials/totes, dishes - Brian, Olivia

Prep LTAR and LTER gas chambers, install T7 and T7 microplot chambers - Slater, Trajana

Prepared 1M KCL, Reconditioned Cadmium Column.

Lachat run LTER T3 soil 10/21/2024.

Sieved LTER soil - Alyssa, Slater, Brian, Trajana, Andie

First 2025 LTER soil sampling, two 0-25cm cores NE of each station, did not sample T6 since flags had been pulled and switchgrass is still not harvested - Alyssa, Slater, Brian, Trajana, Andie

Replace T7 station flags, fix all other LTER, BCSE, LTAR station flags that were leaning or fallen -

Weigh and grind plants, pulverize BCSE deep core soil - Slater, Trajana, Brian, Olivia

Lachat run LTAR soil 9/30/2024.

Finish filtering LTAR leachate, dishes - Brian

Weigh and grind plants, archive air-dried soil, pulverize BCSE deep core soil - Slater, Trajana, Brian, Olivia

Acid washed dishes.

Lachat run GLBRC MLE WN soil (Rhinelander) 10/16/2024.

Filter LTAR soil extracts - Brian, Trajana

GC run BCSE20250307S1BGC had one series of vials submitted out of numerical order. All vials run on the GC in numerical order, but submitted series were …60, 62, 61, 63, 64, 65, etc… (so just 61 and 62 submitted out of order, but subsequently run in numerical order).

Collect LTAR leachate - Brian, Trajana

Weigh LTAR soil for moisture and N-extraction -

Weigh and grind plants, pulverize BCSE deep core soil, subsample/archive SWF deep core soil - Slater, Trajana, Brian, Olivia

Prepared standards, prepared 1M KCL.

Lachat run CLE inorganic N (Hammond) June 2024 soil.

Evacuate LTAR lysimeters -

GC run LTER20250306S1BGC: samples 241 and 242 were switched out of order in the trays submitted by the techs (likely just an oversight). Vials all run in numerical order on GC.

First LTAR soil sampling for 2025, two 0-25cm cores per station (NE of station) - Alyssa, Slater, Trajana, Brian

Weigh ASP6 biomass sampled on 3/12/2025 - Stacey

Sieve LTAR soil - Alyssa, Slater, Trajana, Brian

Weigh and grind plants, pulverize 2022 BCSE deep core soil - Slater, Trajana, Brian

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed dishes.

Weigh and grind plants, pulverize 2022 BCSE deep core soil (Alyssa trained), subsample and archive SWF deep cores soil - Slater, Brian, Andie, Trajana, Olivia

Weigh and grind plants - Brian, Olivia

Subsample and archive SWF DC soil - Slater

LTAR Series 1 gas sampling - Slater, Brian, Olivia

LTAR Hydrosense - Alyssa

Lachat run LTAR soil 8/28/2024.

Reconditioned Cadmium Column.

LTAR gas chamber prep, pull ASP4 microplot chambers and install in ASP3 microplots, sample ASP6 biomass to approximate what could be harvested… from each station, 1.5mN, 0mW to SE corner of 2 x 0.5m frame, plants cut at 6 inches above the ground with electric trimmers, bagged as UNSRT, if plants were lodged or lying down - they were not stood up for cutting. - Slater, Brian, Andie, Trajana

Finish filtering LTER leachate, prep LTAR gas vials and totes - Alyssa

Weigh and grind plants - Andie, Brian

Subsample and archive SWF DC soil - Trajana, Slater

Lachat run LTER Soil 9/17/2024.

Measure and filter LTER leachate - Brian, Trajana, Olivia

Weigh SWF deep core moisture samples - Slater

Collect LTER leachate - Slater, Brian

Pull T7 flags and gas frames for burning - Olivia, Trajana

Burn all T7 plots - Kevin, Sven, Dave, Stacey, Alyssa, Olivia, Trajana, Brian, Slater, plus several others (about 16 total)

Weigh and grind plants - Trajana, Slater

Weigh SWF deep core moisture samples - Andie, Brian

Evacuate LTER lysimeters (first for 2025) - Slater, Trajana, Brian, Andie

Acid washed dishes.

Lachat run LTAR soil 7/23/2024

Finish processing SWF deep cores - Slater, Olivia

Weigh and grind plants - Trajana, Brian

BCSE Series 1 gas sampling - Brian, Slater

Hydrosense in LTER and BCSE - Kevin trained Alyssa (MCSE and BCSE) and Stacey (DF)

Evacuated LTER lysimeters that were installed in fall 2024 since conditions were very dry many did not yield leachate in November or December 2024. These lysimeters were evacuated on 3/7/2025 and left over the weekend. The lids were completely off from DF3-1 and DF3-6. On 3/11/2025 they were checked for pressure, the volume of leachate approximated and then the leachate dumped before re-evacuating with the regular sampling. - Stacey

Lysimeter, pressure, volume from 3/11/2025; CF1-1, N, 0; CF1-3, N, 0; CF1-6, N, 0; CF2-1, N, 20; CF2-3, Y, 300; CF2-6, Y, 200; SF3-1, Y, 600; SF3-3, Y, 450; SF3-6, Y, 200; DF3-1, Y, 350; DF3-3, Y, not recorded; DF3-6, N, not recorded; CF3-1, Y, 50; CF3-3, N, 50; CF3-6, Y, 200

Prepared Standards, Acid washed dishes.

Lachat runs LTAR soil 5/28/2024 and 6/24/2024.

Process SWF deep cores - Brian

LTER Series 1 gas sampling (last winter sampling) - Slater, Olivia, Trajana

Weigh and grind plants - Trajana, Slater, Brian, Andie

Process SWF deep cores - Andie, Olivia, Slater

Lachat run LTER soil 8/14/2024.

Measure and filter LTAR leachate, dishes - Slater, Olivia, Trajana

Process SWF deep cores - Brian

Collect LTAR leachate - Slater, Olivia, Brian, Trajana

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Evacuate LTAR lysimeters (first for 2025), ASP6R4/ASP2R4 box was frozen shut and couldn’t be opened, so those lysimeters were evacuated on 3/4/2025 and collected 3/5/2025. - Slater, Brian, Andie, Trajana

Weigh and grind plants - Slater, Brian

Process SWF deep cores - Trajana

Grind BCSE plants on campus - Andie, Trajana

Dump 2024 LTER leftover plants and 2023 soil samples - Brian, Slater

Reconditioned Cadmium Column, Acid washed all Channel 2 reagent containers.

Lachat run LTER soil T4 T21 7/8/2024.

Weigh and grind plants - Slater, Trajana

Process SWF deep soil cores - Brian

Replaced weather station battery at Hancock. Tagged instrumetations and some sensors.

Almost no snow.

Acid washed dishes, Lachat run LTER soil T3 T21 7/8/2024.

Replaced the weather station batteris at Escanaba and Rhinelander. Tagged instruments and some sensors with barcodes.

Minimal snow at either location.

Weigh and grind plants - Trajana, Brian

Process SWF deep soil cores - Andie, Slater

Finish measuring trees - Slater, Trajana, Andie

Prepared Standards, Lachat run LTER Soil 7/17/2024

Replaced the Battery at the Lake City MLE weather station and logger 125. Labeled sensors with qrcodes.

Snow depth was 15 inches at the snow depth sensor.

Measure CF3 trees - Slater, Trajana

Grind BCSE plants on campus - Alyssa, Brian

Grind BCSE plants on campus - Alyssa, Andie

Weigh and grind plants - Trajana, Brian

Weigh and grind plants - Brian, Trajana

Process SWF deep soil cores - Slater, Andie, Olivia

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Acid washed all reagent containers from Channel 1.

Weigh and grind plants - Slater, Trajana

Process SWF deep soil cores - Brian,

Lachat run LTAR Lysimeter 07/10/2024.

Vial ASP5 R1 S incorrectly labeled (listed as not enough liquid to be filtered). Ran as sample ASP5 R2 S as sample was missing from box. All vials from ASP1 R1 (N, C, S) missing from box, ran as blanks.

Weigh and grind plants - Andie, Brian, Slater

Process SWF deep soil cores - Slater, Trajana, Andie

Acid washed dishes.

Lachat run LTER Lysimeter 06/05/2024.

Weigh and grind plants -

Process SWF deep soil cores -

Weigh and grind plants -

Process SWF deep soil cores -

CPR/First aid training - Alyssa, Slater, Brian, Trajana, Andie

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Lysimeter 05/21/2024.

Weigh and grind plants -

Process SWF deep soil cores -

GC run LTAR20250211S30BGC vial order issue. All vials run in numerical order. Vials submitted in trays out of order as follows: …103, 104, 108, 105, 106, 107, 109… and …111, 112, 114, 113, 115, 116, 117…

Prepared Standards. Lachat run LTER Lysimeter 05/06/2024.

Replaced Lachat reagent lines.

Weigh and grind plants - Brian, Trajana, Olivia

Process SWF deep soil cores -

Replaced Cadmium column. Lachat run LTER Lysimeter 04/15/2024.

Weigh and grind plants - Brian, Trajana

Process SWF deep soil cores - Brian

LTAR Series 30 gas sampling - Brian, Trajana, Olivia

Lachat run LTER Lysimeter 04/01/2024

Weigh and grind plants - Brian, Trajana

Check LTAR gas chambers ahead of 2/11 sampling -

Prepared Standards, Reconditioned Cadmium Column.

Lachat run LTER Lysimeters 03/14/2024.

Weigh and grind plants - Trajana, Brian, Andie

Process SWF deep soil cores - Brian, Trajana

Weigh and grind plants - Trajana, Slater, Brian

Process SWF deep soil cores - Brian, Andie, Slater

Acid washed dishes. Lachat run LTER T3 soil 7/08/2024.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Measure trees (CF, DF) - Slater, Brian, Trajana

BCSE Series 20 gas sampling - Brian, Slater

Weigh and grind plants - Andie, Slater, Trajana

Process SWF deep soil cores - Brian, Trajana

Acid washed dishes, Lachat run LTER T4 soil 7/08/2024.

Reconditioned Cadmium Column. Lachat run LTER T-21 soil 7/01/2024.

LTER Series 16 gas sampling - Trajana, Slater, Brian, Olivia

Weigh and grind plants -

Process SWF deep soil cores -

Measure trees (CF, DF) - Alyssa, Slater, Trajana, Andie

Weigh and grind plants - Andie, Slater, Trajana

Process SWF deep soil cores - Brian, Trajana, Andie, Olivia