Grind LTER plants - Olivia, Brian

LTER gas vial and tote prep - Brian

T7 microplot plant archive data entry - Jon

Grind BCSE plants on campus - Jon, Brian

Grind LTER plants - Sarah

Grind BCSE plants on campus - Alyssa, Jon

Grind LTER plants - Brian, Olivia

BAF subsampling - Slater

Pack CN - Slater, Brian

Weigh SBE plants, grind LTER plants - Slater, Brian

T7 microplot plant archive data entry - Jon

Pack CN - Olivia

Lachat run LTER soil 20250714.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Soil 20250616.

Weigh SBE plants, grind LTER plants - Sarah, Brian, Slater

T7 microplot plant archive - Jon

Pack CN - Slater, Brian

Changed the weather station battery and adjusted solar panel angles at all logger boxes in Lake City. Also checked snow depths at all MLEs during this trip.

Changed MLE weather station batteries and solar panel angles at Rhinelander and Escanaba. Checked for data connections at each logger box.

Weigh SBE plants - Slater, Jon

Grind LTER plants - Jon, Slater

Pack CN - Olivia

Reconditioned Cadmium Column, Acid washed dishes.

Lachat run LTER T4 soil 20250527

Weigh SBE plants - Brian

Grind LTER plants - Slater, Sarah

T7 microplot plant archive - Jon

Data entry, BAF subsampling - Sarah, Slater

Installed new battery at the Hancock weather station, changed all solar panel angles and ensured connections between loggers were active.

Replacement dilutor test against hand dilutions prepared last week to test for accuracy - consistent.

Prepared Standards. Lachat run LTER Soil 20250602.

Grind LTER and weigh SBE plants - Brian

Lachat runs GLBRC soil 20250623, LTAR soil 20250709 (diluted by hand).

Reconditioned Cadmium Column

Weigh plants - Brian

Grind plants - Olivia, Slater

Continue checking T7 microplot plant archive - Jon

Pack CN - Slater

Grind and weigh plants - Slater, Sarah, Brian

Check LTER T7 microplot plant archive jars going back to the start of the project against a spreadsheet created from ANPP data to establish a T7 microplot plant archive data set similar to what we already have for the LTER main plots. - Jon

Pack CN - Slater, Sarah

Prepared standards (hand diluted)

Lachat run GLBRC soil 20250522 (re-ran part of run from 12/22/2025 to compare samples and hand dilution standards to dilutor standards- consistent)

Grind plants - Slater, Jon

Weigh plants - Brian

Data entry - Olivia

Pack CN - Jon, Slater

Grind plants - Brian, Slater

Finish SWF BAF subsampling - Brian

Weigh plants - Jon

Data entry - Olivia

Pack CN - Alyssa

Main lab and field lab clean-up -

LTAR Series 31 gas sampling - Alyssa, Slater, Jon

Weigh plants - Alyssa, Brian

Grind plants - Jon, Alyssa

LTAR gas vial, tote prep - Olivia

BAF subsampling SWF soil - Brian, Slater

Data entry - Slater, Jon

Replaced the weather station logger with a CR1000x. I updated the OS to version 8.04. The station was back in operation around 16:00.

Added external antenna on lux arbor switchgrass tower

Grind plants - Alyssa, Slater

Pulverize SWF deep core soil - Slater, Alyssa

Start subsampling SWF soil for BAF - Alyssa

Data entry - Brian

Weigh plants - Sarah

GC run LTER20250112S17BGC had a vial set shift on one sheet. This was recorded on the sheet and at data entry. All vials run in sequential order on the GC. This is just for reference in case you want to verify that results line up with sites (but again, this should have been fixed on tech data entry of sampling forms): vial set 5,6,7,8 shifted to slots 9,10,11,12; vial set 9,10,11,12 shifted to slots 17,18,19,20; and, vial set 17,18,19,20 shifted to slots 5,6,7,8.

The CR1000 at the weather station is no longer responding even when directly (serial port) plugged into a computer. The power seems ok and the radio blinks normally. I will need to replace the logger.

BCSE Series 24 gas sampling - Brian, Sarah

Grind plants - Brian, Slater

Pulverize SWF deep core soil - Slater, Alyssa

Data entry - Alyssa, Brian

Pack CN - Olivia

removed radios from T7R3 and T7R5.

installed morningstar controller at Lux weather station. I hooked up the cell modem to the load and left the rest of the system connected to the CH150, The battery is connected to both the Morningstart controller and the CH150. The morningstar controller is hooked up to the solar panel, so it acts as the charge controller. the CH150 only acts as a regulator.

Data entry - Slater, Brian, Sarah

LTER Series 17 gas sampling - Slater, Brian, Sarah

mcse loggernet network was in a jabber state. I shut down the T7 R6 and R4 radios and it cleared up. I removed the radios from R2, R6 and R4, since the sensors were out.

Data is flowing again

Measure trees in CF3. Done measuring trees for this year. - Slater, Brian, Sarah, Ethan, Jarrod

Check all gas chambers (LTER, GLBRC, LTAR) -

Measure trees in DF2, CF2 and DF3 - Slater, Sarah, Brian, Ethan, Jarrod

Weigh and grind plants - Alyssa, Olivia, Brian

Measure trees in DF1 and DF2 - Alyssa, Sarah, Brian, Ethan, Jarrod, Olivia

Weigh plants - Olivia, Alyssa

Finish BAF subsampling, blow out Torit filters, grind plants - Slater

Pack CN - Brian

BAF BCSE plants subsampling - Olivia

Weigh plants - Slater

Data entry, WPS training - Alyssa

Measure trees in DF1 - Alyssa, Olivia, Brian, Ethan, Jarrod

Measure trees in CF1 and DF1 - Alyssa, Slater, Olivia, Sarah, Brian

Weigh and grind plants - Sarah, Slater

Pack CN - Olivia

Weigh and grind plants - Brian, Olivia

Finish weigh roots, BAF subsampling - Jon

Pack CN - Slater, Jon

Acid washed dishes.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run GLBRC 20250430, part of 20250522 (dilutor malfunction - refroze all samples to run at a later date)

Weigh and grind plants - Sarah, Jon

Pulverize SWF soil -

Weigh roots, pack CN - Slater, Olivia

Final forest leaf litter collection, put away screens, bungees and clips, flip traps over -

Acid washed all Channel 1 reagent containers. Replaced all reagent tubing.

Weigh plants - Brian

Grind plants, pulverize SWF 2024 deep core soil - Slater, Olivia

Weigh 2024 Switchgrass Nitrogen Rate Experiment deep core roots - Jon

Weigh plants - Brian, Slater

Grind plants - Jon, Olivia

BAF subsampling - Olivia

Weigh 2023 Switchgrass Biodiversity Experiment deep core roots - Brian, Jon

Pack CN - Slater

Acid washed dishes, reconditioned Cadmium column.

Lachat run LTER T3 soil 20250527.

Lachat run LTAR soil 20250609. Notes from run (also noted when uploaded):

• Vials ASP3 R4 B and ASP3 R5 B swapped in order in box. Ran as labeled but may have been labeled incorrectly due to results.
• 3 sets of Vials may have been labeled incorrectly due to results, ran as labeled:

ASP3 R2 A and ASP3 R3 A

BAU1 R4 C and BAU2 R4 C

BAU1 R5 C and BAU2 R5 C

(discounted vials when uploading due to large CV)

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Acid washed dishes.

LTAR 30 gas sampling - Slater, Sarah, Jon

Pack CN - Stacey, Olivia, Brian

Weigh plants - Brian, Olivia

Grind plants - Slater

BAF subsampling (BCSE harvest 2021) - Sarah

Weigh 2023 Switchgrass Biodiversity Experiment deep core roots - Jon

Lachat run MDARD Soil September.

Check LTAR gas chambers - Slater

BCSE 23 gas sampling - Olivia, Jon

Pack CN - Stacey, Slater

Weigh plants - Jon

Grind plants - Olivia

LTAR gas vial, tote prep - Slater

BAF subsampling (BCSE harvest 2020) - Brian

Check BCSE gas chambers - Jon

Pack CN - Brian, Olivia

Weigh plants - Olivia, Brian

Grind plants - Sarah, Slater

BAF subsampling (BCSE harvest 2019) - Slater, Jon

BCSE gas vial, tote prep - Jon

Dishes -

Prepared Standards, Acid washed dishes and all Channel 2 reagent containers.

Pack CN - Slater, Brian

Weigh plants - Brian, Olivia

Grind plants - Slater

Lab clean-up, dump soil buckets, weigh dry tins - Sarah

LTAR gas data entry - Jon, Olivia

Archive LTAR air-dried soil -

Lachat run LTAR Lysimeter 20251210.

All 2025 LTAR Lysimeter Lachat samples run and uploaded

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Finished dilutions from Lachat run LTAR Lysimeter 20251124.

Reconditioned Cadmium Column

Acid washed dishes.

Filter LTAR leachate; collection included many more samples than 11/24-25, so those archive samples were discarded and this collection was archived in it’s place. Also, subsamples from ASP3 and BAU2 leachate were delivered to Younsuk Dong and Subhasis Giri on campus by Dave Weed for phosphorus analysis at Brook’s request. - Slater, Sarah

Collect LTAR leachate - Brian, Jon

Measure poplar trees - Slater, Sarah, Jon, Brian

Filter LTAR soil extracts - Slater, Sarah

Prepared standards, Lachat run LTAR Lysimeter 20251124.

Evacuate LTAR lysimeters, boxes needed to be dug out from under the snow - Slater, Brian, Jon, Sarah

Measure poplar trees - Jon, Brian

LTER 16 gas sampling, first winter - Brian, Slater, Sarah, Alyssa, Jon

Finish sieving LTAR soil - Jon, Alyssa

Weigh LTAR soil for extraction and moisture - Jon, Alyssa, Brian, Sarah

LTAR soil sampling; approximated station flag locations in these plots since flags hadn’t been installed: ASP1-3, ASP2-3, ASP2-4, ASP5-4, ASP3-4, BAU1-4, BAU2-3, BAU2-4 - Alyssa, Slater, Brian, Jon, Sarah

LTER gas vial/tote prep, weigh dry soil tins, dishes - Slater, Brian, Jon, Sarah

Start sieving LTAR soil - Alyssa, Slater, Brian, Jon, Sarah

Removed snow from solar panels at Lux Arbor and adjusted panels vertical for the winter. I moved T7R2 to use the RF407 network, and disconnected the CS65X power and ground from T7R4, to let the battery recover I think there is a short in T7R4 since it draws down the battery too quickly.

Checked the battery at T7R5 and it was at 14V (in the sun) I did not see any obvious issues with the wiring. The ground and power from the solar panel were pretty close but I don’t think they were touching. I’ll keep checking to see if this resolves the negative battery V readings.

Dishes, LTAR gas data - Slater, Brian, Olivia, Jon

Filter BCSE subplot soil extracts - Alyssa, Brian, Olivia

T7R5 datalogger is reporting negative voltage. There were calibration errors reported in the station status. I recompiled and restarted the program, to see if it was a temporary glitch due to low battery or something else.

Finished Dilutions from Lachat run LTAR Lysimeter 20251112.

Collect leaf litter from CF, DF, SF; collect last leaf litter from T5 then pulled/put away traps - Slater, Jon, Brian, Olivia

Weigh BCSE subplot soil for extraction and moisture, plus subsample G1-3 main and subplot to send to A&L - Slater, Jon

LTAR gas data - Brian, Jon

Dishes - Brian, Slater

Prepared 1M KCL. Finished dilutions from Lachat run LTAR Lysimeter 20251021.

Lachat run LTAR Lysimeter 20251112. Notes from run:

-Vial BAU1 R4 C most likely mislabeled as BAU2 R4 C (vial shouldn’t exist)

-Vial BAU2 R2 S most likely mislabeled as BAU2 R1 S (vial shouldn’t exist)

Sieve BCSE subplot soil -

Filter BCSE main soil extracts, dishes -

BCSE subplot soil sampling; G1-3 nine cores/subplot, G4-10 six cores/subplot. This includes G1 non-stover removal area; G2, 3, 4, 5, 6, 7, 9 unfertilized area; G8 historically unfertilized area (south end, between 1st and 2nd rows); and G10 fertilized area. - Alyssa, Slater, Olivia, Brian, Sarah Jon

Adjusted REX and BCSE Solar panels to winter position.

Lachat runs LTAR Lysimeter 20251001, 20251021. Notes from runs:

-20251001 - Vial BAU2 R1 S was missing from box, not run. Unusually high NH4 results for bottle ASP1 R3 N, contaminated?

-20251021 - Vial ASP3 R4 S was missing from box, not run. Vial BAU2 R4 N potentially mislabeled as BAU2 R4 C, or volume may have been listed incorrectly on data sheet. Bottle C is listed as having pressure, but no liquid recorded, where N had no pressure. Ran as vial BAU2 R4 N. No blanks were included, used fresh RO water.

Heavy snow storm over the weekend caused the following errors on both IRGASON instruments: The irgason diagnostic code on both towers is “847873”  CO2 and H2O signals too low and exceeding the limits set.  Bits 0, 12, 13, 14, 15,, 18, 19 are all set. The low reading were due to snow/ice on the CO2 sensor and the sonic. Delicately blew off and removed the snow. Also removed the lower wickings on the sonic anemometers. Need new wicking materials.

Prepared 1M KCL, standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Finish sieving BCSE main soil - Jon, Brian

Weigh BCSE main soil for extraction and moisture - Jon, Brian, Slater

Filter MLE MC soil extracts - Jon, Slater, Brian

GC sequence LTAR20251126S29BGC had two issues with vial order submitted in trays. Vials 100 and 101 were switched in trays, and vials 135 and 136 were switched in trays. All GC vials were run sequentially on instrument.

Install G1 gas frames - Brian

BCSE main soil sampling; G1-3 three cores per station to ensure enough soil to send to A&L, G4-10 two cores per station. - Alyssa, Sarah, Olivia, Slater, Jon, Brian

Weigh MLE MC soil (sampled 11/25/2025) for extraction and moisture - Brian, Jon

Sieve BCSE main soil - Alyssa, Sarah, Olivia, Slater, Jon, Brian

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared 1M KCL.

Sequence BCSE20251120S22BGC had a couple vial submission issues.
Duplicate 1 was swapped with Sample 1 in field tray. Vials 5 and 6 were swapped (out of order) in tray. Samples run in this order: Duplicate 1, 2, 3, 4, 5, 6,… …., 79, 80, Air1, Air2, Air3, Air4, 1, Dup2, Dup3, Dup4.

LTAR Series 29 gas sampling - Slater, Jon, Brian

LTAR Hydrosense - Alyssa

Sieve MLE MC Lake City soil, sampled 11/25/2025; dishes, weigh dry soil tins - Brian, Jon, Slater, Sarah

Sample BCSE soil respiration jars - Sarah

Lachat runs LTAR Lysimeter 20250728, 20250821, and 20250915.

LTAR gas vial, tote prep -

Filter MLE WC Hancock soil extracts, dishes -

Measure, and filter LTAR leachate, dishes -

LTAR gas chamber prep - Slater

Collect LTAR leachate - Brian, Jon

Sample MLE MC Lake City soil - Todd, Chris

Acid wished dishes. Lachat run LTAR Lysimeter 20250714: BAU1 R4 C bottle was spilled, no samples were filtered/run.

Weigh MLE WC Hancock soil for extraction and moisture - Brian, Sarah

Dishes - Brian, Sarah

Sample BCSE soil respiration jars - Sarah