Projectlog Entries List

GC Sequence LTAR20260312S1BGC had a few samples submitted out of order. All samples run in numerical sequence on GC. Samples Dup3 and Dup4 were swapped with vials 3 and 4 as submitted. They were swapped back in proper order on GC run. Another sample out of order towards the end as such: ….112, 116, 113, 114, 115, 117, 118….

Pack LTAR 2023 CN - Olivia

Dishes - Jon, Olivia

Grind SBE 2025 - Brian

Relabel 2019, 2021 BCSE G2 harvest archive jars - Jon

Lachat run GLBRC Soil MLE WN 2025118 (Rhinelander)

• G6R2 E a and G6R2 W a vials may have been swapped, ran in vial box order.

Inventory LTER leachate archive - Brian

LTAR Series 1 gas sampling - Olivia, Jon, Brian

Dump leftover plant samples (researcher stored samples, LTAR 2025) - Olivia, Jon

Dishes, grind SBE palnts, inventory LTER leachate samples - Sarah, Jon, Brian

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Collect LTAR leachate - Brian, Jon

Prepared Standards, Lachat run GLBRC MLE MN soil 20250123 (Escanaba)

Grind SBE plants - Olivia, Brian

Measure and filter LTAR leachate, save archive sample, dishes - Olivia, Jon

Lachat run GLBRC MLE MS soil 20251118 (Lux Arbor).

Grind SWF plants - Sarah, Jon

Evacuate LTAR lysimeters - Brian, Jon

Dump soil, clean up field sites, install G1 station flags - Sarah, Brian

Grind LTAR 2025 plants - Sarah, Jon

One last check on LTER T7 microplot plant archive samples where ANPP records don’t match Archive records - Jon

Pack LTAR 2023 plant CN - Olivia

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER 20251117.

Check LTAR gas chambers, pull out ASP3 no compost chambers and install them in ASP2 no compost microplots based on GPS file - Jon, Olivia

Grind LTAR 2025 plants - Sarah

Pack LTAR 2023 plant CN - Jon

Prep LTAR gas vials - Olivia, Alyssa

Lachat run LTER soil 20251015.

GC sequence BCSE20260303S1BGC notes: All samples run in sequential order. One set submitted out of order in trays as follows: vial # ….44, 45, 47, 46, 48, 49….

LTER Series 1 gas sampling (last winter sampling) - Jon, Alyssa, Dave

Grind LTAR plants - Sarah

Acid washed dishes, Replaced Cadmium column.

BCSE Series 1 2026 gas sampling - Jon, Olivia

Prep LTER gas vials, grind LTAR plants - Jon

Prep BCSE gas vials and totes - Jon

Check BCSE and LTER gas chambers - Jon

Prepared standards.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store

Lachat run LTER soil 20250820 (Re-extracted on 2/24/2026 due to KCL contamination)

• 1 bin of soil bags from set was in a malfunctioning walk-in cooler (90 degrees). Noted below are those bags: T4 R1-R3, R5, T5 R1-R6, T6 R1-R6, T7 R1-R6

Grind LTAR 2025 plants - Brian, Jon

Pack LTAR 2023 plant CN - Jon

Dump finished (nitrate and ammonium uploaded and checked) soil samples -

Pull G8 flags for tree harvest soon, dump soil samples - Olivia, Brian

Grind LTAR 2025 plants - Olivia

Pack LTAR 2023 plant CN - Brian

Relabel BCSE 2021 archive jars - Jon

Filter 8/20 soil extracts, dishes - Jon, Brian

Grind LTAR 2025 plants - Jon

Reweigh LTER 8/20/2025 soil samples for extraction and moisture, initial results were good but reextracted to compare to other ammonium contaminated samples. Also, some of this soil warmed up when a walk-in stopped working, so interesting to compare initial to warmed samples. - Brian, Sarah

Relabel BCSE 2021 archive jars - Brian

Weigh dry soil tins, wash dishes - Sarah

Prepared 1m KCL

Lachat run LTER soil 20250806 (Re-extracted on 2/23/26 to compare to contaminated samples)

Grind LTAR 2025 plants - Brian

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Jon

Filter 8/6 soil extracts - Jon, Brian

Lachat run LTER T4 soil 20250723 (Re-extracted on 2/18/26 due to KCL contamination)

Prepared 1M KCL

Lachat run LTER T3 soil 20250723 (Re-extracted on 2/18/2026 due to KCl contamination)

Grind LTAR 2025 plants - Jon, Sarah

Reweigh LTER 8/6/2025 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts, results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Brian, Sarah

Relabel BCSE 2019, 2021 archive jars - Jon

Weigh dry soil tins, wash dishes - Brian

Acid washed dishes, reconditioned cadmium column.

Lachat run LTAR 20251103.

Reminder, tower two has only two sets of ground sensors. Will replace all but the heat flux plates this spring.

Reinserted all heat flux plates, temp sensors, and CS650s. Tower 1 has one CS650 with a chewed cable near the sensor.

Tipping bucket on tower 2 was not fully horizontal. There was one tip during the repair.

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Brian

Prepared Standards, Replaced all reagent tubing.

Lachat run LTAR 20251008.

Grind LTAR 2025 plants - Jon

Pack LTAR 2023 plant CN - Olivia

Relabel BCSE 2019, 2021 archive jars - Brian

Filter 7/23/2025 soil extracts - Jon, Brian

Lachat run LTER T4 soil 20250929.

Prepared 1M KCL.

Grind LTAR 2025 plants - Sarah

Reweigh LTER 7/23/2025 T3, T4 soil samples for extraction and moisture, due to ammonium contamination detected in the original extracts; results will be compared to original for both nitrate and ammonium, and determined if new ammonium data should be kept with a note. - Jon, Brian

Dumped leftover BCSE 2025 plants, then started dumping finished soil samples - Brian, Sarah

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER T3 Soil 20250929.

Finish grinding BCSE 2025 plants, start grinding LTAR 2025 plants - Olivia, Brian

Pack LTAR 2023 plant CN - Jon

Cleaning at field lab and trucks, start relabeling BCSE 2019 plant archive jars with labels Nick Thrower sent with vertical barcodes so scanner can read them (they were horizontal and went around the corner of the jars, so couldn’t be read by a scanner) - Brian

Finish dumping LTER leftover plants - Sarah

Grind BCSE plants - Jon

Pack LTAR plant 2023 CN - Brian

Finish subsampling SWF plants for BAF, cleaning - Sarah

Acid washed dishes. Lachat run LTER Soil 20250915.

Lachat run LTER Soil 20250820 - KCL Contamination in Channel 1 blanks (NH4 results). Not uploaded for now.

Grind BCSE plants - Brian

Finish packing LTER plant 2023 CN, start packing LTAR 2023 plants - Jon, Olivia

Pick up LTAR field samples from Josh, dump LTER leftover plants - Jon

LTAR tower 1 had irgason errors. Checked around 2:30PM and found bird droppings on the lower lens. Cleaned with a KIM Wipe. Now reading fine.

Checked other sensors. all look good.

Lachat run LTER soil 20250806.

Measured the dimensions of the various towers LxWxH KK, DW Set 1 (Vizza Roley) 14 x 16 Single riser 64”. New wheels ground to top of riser is approx. 80” Qty 4 This is the only set with a solid riser.

Set 2 (Vizza/Roley) 14x16’ 2’ +3’ riser ground to top approx same as above if we change the wheels to new type. Qty 4

Set 3 12’ x 12’ joint to top of riser is approx 36-40” (snow). Riser ground to top is 57ish” Qty 4

Set 4 old 14x16’ ground to riser top is 52” If set 3 and 4 had same wheel type height would be equiv. Both use 3’ riser.

Set 5 ALL REX Shelters 14x18’

GC Sequence LTER20260206S18BGC notes. Vials 13-16 were not sampled (techs couldn’t find chamber in the snow), but run on GC as empty vials to keep run in order. Tech notes that vials 73-112 contained swapped sets (i.e. vial 93-96 were used for sheet slots 73-76). These were corrected on the field note sheets and entered at the field note data entry to properly line up the vials with the sites

Grind BCSE plants - Jon

Pack LTER plant 2023 CN - Brian

GC Sequence BCSE20260205S25BGC vials submitted out of sequence by techs. All vials run in numerical sequence on GC. Vials submitted out of sequence as follows: …31,32,34,33,35,36… …50,51,56,53,54,55,52,57,58….

Grind BCSE plants - Brian

Pack LTER plant 2023 CN - Jon

Prepared Standards, Lachat run LTAR soil 20250908.

Grind ASP6 POACEAE (combined grasses) on campus, deliver BCSE grain harvest samples to Chris - Jon, Brian

Grind BCSE plants - Olivia

Acid washed dishes, reconditioned cadmium column.

Lachat run LTAR soil 20250813.

Grind BCSE plants - Sarah

Pack CN - Brian

Sort ASP6 samples to grind on campus 2/10/2026 - Jon

Prepared Ammonium Chloride Reagent (4L): 320 gms Ammonium Chloride 4.0 gms EDTA pH to 8.45 with NaOH, store.

Lachat run LTER T4 T21 Soil 20250723.

• Samples T4R2S4, T4R4S1, and T4R4 strip were not processed, not included.

LTAR Series 32 gas sampling - Brian, Jon, Sarah

Acid washed dishes.

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

LTER Series 18 gas sampling - Alyssa (am), Jon, Brian, Olivia (pm)

Check LTAR gas chambers ahead of 2/9/2026 sampling -

BCSE Series 25 gas sampling - Jon, Brian

Check LTER gas chambers ahead of 2/6/2026 sampling - Olivia

Finish grinding LTER plants -

Prepare LTAR gas vials and totes -

Lachat run LTER T3 T21 soil 20250723

• No T3R1S1 samples were processed, not included.

Check BCSE gas chambers ahead of 2/5/2026 sampling - Jon

Grind BCSE plants on campus - Brian, Sarah

Prepare BCSE gas vials and totes - Alyssa

Finish entering T7 microplot plant archive data - Jon

Lachat run LTER T21 Soil 20250714

• No CF1 samples were processed, not included.

Grind LTER plants - Olivia, Brian

Prepare LTER gas vials and totes - Brian

T7 microplot plant archive data entry - Jon

Acid washed dishes, prepared Standards.

Prepared fresh Phosphate Buffer (1L) 28 gms Sodium Hydroxide pellets 50 gms sodium phosphate dibasic heptahydrate dilute to 1 L with nanopure.

Prepared EDTA Reagent (1L) 66 gms ethylenediamine tetraacetic acid disodium salt dihydrate (EDTA) Approximately 7.0 gms sodium hydroxide pellets ph to 7.03 with NaOH pellets then dilute to volume with nanopure.

Lachat run LTER T4 soil 20250723. Contamination in KCL blanks in NH4 results, not uploaded.

Grind BCSE plants on campus - Jon, Brian

Grind LTER plants - Sarah

Lachat run LTER T3 Soil 20250723. KCL NH4 contamination in blanks. Not uploaded.

Grind BCSE plants on campus - Alyssa, Jon

Grind LTER plants - Brian, Olivia

BAF subsampling - Slater

Pack CN - Slater, Brian

Weigh SBE plants, grind LTER plants - Slater, Brian

T7 microplot plant archive data entry - Jon

Pack CN - Olivia

Lachat run LTER soil 20250714.

Weigh SBE plants, grind LTER plants - Sarah, Brian, Slater

T7 microplot plant archive - Jon

Pack CN - Slater, Brian

Prepared Color Reagent (2 L) 200 ml phosphoric acid 80 gm sulfanilamide, 2.0 gm N-1-naphthylethylenediamine dihydrochloride, Dilute to 2L with Nanopure H2O.

Lachat run LTER Soil 20250616.

Changed the weather station battery and adjusted solar panel angles at all logger boxes in Lake City. Also checked snow depths at all MLEs during this trip.

Changed MLE weather station batteries and solar panel angles at Rhinelander and Escanaba. Checked for data connections at each logger box.

Reconditioned Cadmium Column, Acid washed dishes.

Lachat run LTER T4 soil 20250527

Weigh SBE plants - Slater, Jon

Grind LTER plants - Jon, Slater

Pack CN - Olivia

Installed new battery at the Hancock weather station, changed all solar panel angles and ensured connections between loggers were active.

Replacement dilutor test against hand dilutions prepared last week to test for accuracy - consistent.

Prepared Standards. Lachat run LTER Soil 20250602.

Weigh SBE plants - Brian

Grind LTER plants - Slater, Sarah

T7 microplot plant archive - Jon

Data entry, BAF subsampling - Sarah, Slater

Grind LTER and weigh SBE plants - Brian

Weigh plants - Brian

Grind plants - Olivia, Slater

Continue checking T7 microplot plant archive - Jon

Pack CN - Slater

Lachat runs GLBRC soil 20250623, LTAR soil 20250709 (diluted by hand).

Reconditioned Cadmium Column

Grind and weigh plants - Slater, Sarah, Brian

Check LTER T7 microplot plant archive jars going back to the start of the project against a spreadsheet created from ANPP data to establish a T7 microplot plant archive data set similar to what we already have for the LTER main plots. - Jon

Pack CN - Slater, Sarah

Grind plants - Slater, Jon

Weigh plants - Brian

Data entry - Olivia

Pack CN - Jon, Slater

Prepared standards (hand diluted)

Lachat run GLBRC soil 20250522 (re-ran part of run from 12/22/2025 to compare samples and hand dilution standards to dilutor standards- consistent)

Grind plants - Brian, Slater

Finish SWF BAF subsampling - Brian

Weigh plants - Jon

Data entry - Olivia

Pack CN - Alyssa

Main lab and field lab clean-up -

LTAR Series 31 gas sampling - Alyssa, Slater, Jon

Weigh plants - Alyssa, Brian

Grind plants - Jon, Alyssa

LTAR gas vial, tote prep - Olivia

BAF subsampling SWF soil - Brian, Slater

Data entry - Slater, Jon